Postinfection activity of synthetic antimicrobial peptides against Stemphylium vesicarium in pear
dc.contributor.author
dc.date.accessioned
2015-11-24T14:28:54Z
dc.date.available
2015-11-24T14:28:54Z
dc.date.issued
2014
dc.identifier.issn
0031-949X
dc.identifier.uri
dc.description.abstract
Brown spot of pear is a fungal disease of economic importance caused by Stemphylium vesicarium that affects the pear crops in Europe. Due to the characteristics of this disease and the moderate efficacy of available fungicides, the effectiveness of control measures is very limited; however, synthetic antimicrobial peptides (AMPs) may be a complement to these fungicides. In the present study, 12 AMPs of the CECMEL11 library were screened for fungicidal activity against S. vesicarium. In vitro experiments showed that eight AMPs significantly reduced the germination of conidia. The most effective peptides, BP15, BP22, and BP25, reduced fungal growth and sporulation at concentrations below 50 μM. Leaf assays showed that preventive application of BP15 and BP22 did not reduce infection; however, when the peptides were applied curatively, infection was significantly reduced. The use of a BP15 fluorescein 5-isothiocyanate conjugate revealed that the peptide binds to hyphae and germ tubes and produces malformations that irreversibly stop their development
dc.description.sponsorship
This research was supported, in part, by grants from the Ministerio de Educacion y Ciencia (AGL2009-09829/AGR) of Spain and the Comissio Interdepartamental de Recerca i Tecnologia of the Generalitat de Catalunya (2009SGR00812). M. Puig was the recipient of a predoctoral fellowship from Universitat de Girona (BR10-17)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
dc.relation
info:eu-repo/grantAgreement/MICINN//AGL2009-09829/ES/Desarrollo Y Evaluacion De Estrategias Basadas En Agentes De Biocontrol Y Peptidos Antifungicos Para El Control Integrado De La Estemfiliosis Del Peral/
AGAUR/2009-2014/2009 SGR-812
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1094/PHYTO-02-14-0036-R
dc.relation.ispartof
© Phytopathology, 2014, vol. 104, núm. 11, p. 1192-1200
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Articles publicats (D-EQATA)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Postinfection activity of synthetic antimicrobial peptides against Stemphylium vesicarium in pear
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
021419
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1943-7684