2024-03-29T15:16:24Zhttp://dugi-doc.udg.edu/dspace-oai/requestoai:dugi-doc.udg.edu:10256/112362015-10-14T00:01:02Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Zamora Hernández, Lluís
Busquets Casas, Laia
Universitat de Girona. Facultat de Ciències
2015-10-13T08:39:45Z
2015-10-13T08:39:45Z
2015-06
http://hdl.handle.net/10256/11236
Salmo trutta is one of the most studied species in Europe, however, the populations that live in the lakes of the Pyrenees are little known. The most outstanding fact of this lack of knowledge is the ignorance that these fish populations are not native of these habitats and, therefore, they are one of the major causes of the decline of biodiversity of these ecosystems.
LIFE + LIMNOPIRINEUS, that began the summer of 2014 and that is going to run for five years, it has the objective of improving the conservation status of species and aquatic habitats in the high mountains of the Pyrenees. The removal of this non-native species is one of the actions to achieve this improving and with the sampled individuals from the lakes it has been possible to do the next research that have the aim to realize a characterization of the populations of Salmo trutta present in the high mountain lakes of the Pyrenees.
This characterization is done by the measurements of the furcal length and the determination of the ages of each individual by the otoliths reading, which allow to describe the growth and the survival of the populations present in these habitats by the adjusting of the models Von Bertalanffy and constant survival, respectively. Moreover, has been done an analysis of the body pigmentation pattern of each trout for knowing its linage.
The ages obtained by the otoliths readings show unstructured populations with a poor density of young individuals. The parameters obtained by the growth corves indicated moderate asymptotic lengths compared with others freshwater ecosystems and little differences between the growth rates.
On the other hand, the mortality rates, obtained by the adjustment of the survival constant model, are quite similar between the different lakes. Nevertheless, these little differences can be related with the lakes characteristics like the depth, the surface or the presence of the specie Phoxinus sp.
Finally, the analysis of the body pigmentation pattern shows two different patterns: lakes with dominance of Mediterranean lineage or lakes with a strong presence of Atlantic lineage
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Truita comuna -- Pirineu català
Truites (Peixos) -- Pirineu català
Trout -- Catalan Pyrenees
Biología
Creixement i demografia de les poblacions de l’espècie Salmo trutta introduïdes en diversos estanys d’alta muntanya dels Pirineus
info:eu-repo/semantics/bachelorThesis
east=0.8753833120117633; north=42.64602206064912; name=Estanys d’alta muntanya dels Pirineus (Vilac, Negre de Cabanes, Dellui, i Sarradé)
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oai:dugi-doc.udg.edu:10256/112372015-10-14T00:01:08Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Casadevall, Margarida
Canals Carbó, Xavier
Universitat de Girona. Facultat de Ciències
2015-10-13T08:43:31Z
2015-10-13T08:43:31Z
2015-06
http://hdl.handle.net/10256/11237
The aim of this work was to study the factors that affect the quality and preservation of the
horse mackerel (Trachurus trachurus, F. Carangidae), for the consumer and also in order to
extend its commercial life. The study was divided into three sections: the study of parasites;
the migration process and excapsulation of these parasites after the death of the fish; and
degradation of the fish through the study of quality indices.
Parasitic nematodes belonged to two different genera of F. Anisakidae: Anisakis and
Hysterothylacium. In the case of Hysterothylacium, there were always larvae of the species H.
aduncum: larva 3 (L3) L4 (last stage before the adult) and step 3 to 4 larvae (L3-L4). Regarding
Anisakis, we differentiated between type I and type II level (specific determination requires
molecular studies), always at larval stage 3 (L3). The presence of Anisakis was observed mainly
in medium-sized fish, while H. aduncum was detected more frequently in larger fish.
This differential distribution of parasites was related to a change in diet depending on the size
of fish. Crustaceans being the main prey of young ones and instead, fish, were the dominating
prey on the old ones. Mindful that anisakids nematodes can cause problems in allergic
consumers, it raised the need to know the location of these parasites in the host, not only in
fresh fish but also once time had passed.
Both the analysis of the distribution of the parasites and the excapsulation and migration
process were done with medium-sized individuals. All the parasites were Anisakis type I. In
fresh fish, parasites were observed mainly in the digestive tract. As the days passed, their
presence could be observed, especially, in the visceral cavity. Parasites were never observed
encapsulated in the muscles, but instead, there was penetration of parasites in the meat from
the third day on.
Finally, the "Quality Index Method" (QIM) showed no significant differences in the overall
analysis of all attributes and organoleptic quality of tissues, including the whole fish and the fish
without viscera (gutted). Nevertheless, aspect and colour analyses of the flesh showed
significant differences. Additionally there was a personal test of acceptability, which gave
significant differences between the samples of whole fish and without viscera (gutted)
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Perciformes -- Conservació
Perciformes -- Paràsits
Perciformes -- Parasites
Perciformes -- Conservation
Biología
Els Sorell (Trachurus trachurus): estudi dels paràsits i la seva conservació i durabilitat
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112392015-10-14T00:01:08Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vila i Gispert, Anna
Pou i Rovira, Quim
Cuello Regàs, Sandra
Universitat de Girona. Facultat de Ciències
2015-10-13T08:49:15Z
2015-10-13T08:49:15Z
2015-06
http://hdl.handle.net/10256/11239
In Banyoles Lake there are a lot of exotic species, especially there are a lot of exotic fish that have been introduced by human activity over many years. Exotic fish change original communities. These changes are: trophic relationships, hybridization and genetic alteration, introduction of pests and diseases and global changes in the whole lake ecosystem. However, these introductions are also the cause of the extinction of some native species, such as three-spined (Gasterosteus aculeatus), or there are others, which are in a terrible decrease of theirs populations.
Basically this project studies three exotic species: largemouth bass (Micropterus salmoides), pumpkinseed (Lepomis gibbosus) and perch (Perca fluviatilis). Nowadays, the first and the second ones are the most abundant in the lake and the third one appears in lower amounts. The growth and the demography will be studied in the Life Project Potamo Fauna through the extraction and the reading of otoliths that are a small structure of calcium carbonate that have all teleost fish inside the inner ear. That one being, the data that we will obtain, will be analyzed by a mathematic model that has always been used, Von Bertalanffy model. From the data obtained in the last Life Project (2010-2013), populations of exotic species are compared and then we know if populations have changed or not. Mortality in juveniles of largemouth bass is progressively increasing by the time being, with the exception of the last winter due to the fact that lately it has been fished less intensively. So, largemouth bass population has rejuvenated there are more juveniles fish (0-2 years) than adults, and their longitude have also increased during these projects. It is necessary to maintain it, because there are the biggest fish, which cause a lot of problems to native species, for its big voracity. However, in pumpkinseed population it remains stable over time because of the decline in big largemouth bass, his potential predator and others, such as Sander lucioperca. But, we cannot affirm anything of its demography, because in the beginning of the previous Life Project, the dates were not clear. The only dates that are clear in pumpkinseed population are the obtained this winter (14-15). The demography of Perca fluviatilis could not be studied because we captured only a few and the results are not significant
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application/pdf
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Perca americana -- Catalunya -- Banyoles, Estany de
Perciformes -- Catalunya -- Banyoles, Estany de
Black bass GLC. -- Catalonia -- Banyoles, Estany de
Perciformes -- Catalonia -- Banyoles, Estany de
Biología
Creixement i demografia de la perca americana (Micropterus salmoides), el peix sol (Lepomis gibbosus) i la perca de riu (Perca fluviatilis) a l’Estany de Banyoles
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112422015-10-14T00:01:10Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Briz González, Maria Dolors
Feliu Margalef, Júlia
Universitat de Girona. Facultat de Ciències
2015-10-13T09:07:21Z
2015-10-13T09:07:21Z
2015-06
http://hdl.handle.net/10256/11242
Cryopreservation consists of freezing cells or tissues at very low temperatures using liquid
nitrogen in order to avoid cellular death. Substances called cryoprotectants are used to protect
the cell against thermal shock, ethylene glycol and dimethyl sulfoxide are used among others.
However, as a result of cooling, deleterious effects occur on cells that could compromise their
survival and viability. Cryopreservation is a useful process for assisted reproductive
technologies because this method allows preserving the two types of sex cells, oocytes and
spermatozoa, as well as embryos. Embryo and oocyte cryopreservation becomes important
when female fertility must be preserved, for instance in cases of cancer or autoimmune
diseases and, for women with reproductive dysfunction due to other reasons. Currently, there
are two methods used for embryo and oocyte cryopreservation in the laboratories of clinical
embryology: slow freezing and vitrification. In the first one, cells are exposed to moderate
concentrations of cryoprotectants, both permeating and non-permeating and, a gradual
decrease on temperature is produced up to liquid nitrogen boiling temperature (-196ºC). In
the vitrification procedure, cells are exposed to high concentrations of cryoprotectants and, by
a direct contact with liquid nitrogen, ultrafast freezing is achieved. Embryo cryopreservation is
widely used nowadays, while oocyte cryopreservation is still considered an experimental
technique. This is because the advantages of embryo features in comparison to oocyte; for
example, embryos have a better permeability to cryoprotectants and they are more resistant
to cell damage after cryopreservation. Nevertheless, oocyte cryopreservation has some
advantages in terms of medical handling and financial costs. In addition, the last one does not
imply so many moral, ethical and religious controversies compared to embryo
cryopreservation. The most commonly used cryopreservation method is mainly vitrification,
which provides better success rates than slow freezing. Despite progress made in assisted
reproductive technologies over last years, cryopreservation is still a subject of extensive
research; because there is the purpose to improve the protocols in order to achieve the best
possible results with the maximum security
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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Crioconservació d'òrgans, teixits, etc
Reproducció humana assistida
Embrions -- Criopreservació
Cryopreservation of organs, tissues, etc.
Human reproductive technology
Embryos -- Cryopreservation
Biología
Estat actual de les tècniques de criopreservació en la optimització de la fertilitat femenina
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oai:dugi-doc.udg.edu:10256/112442015-10-14T00:01:15Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Boix Masafret, Dani
Fernández Cortés, Ariadna
Universitat de Girona. Facultat de Ciències
2015-10-13T09:14:48Z
2015-10-13T09:14:48Z
2015-06
http://hdl.handle.net/10256/11244
Cyprinid Phoxinus sp. is a non-native species of high mountain lakes of the Pyrenees. Its introduction has been done by humans, causing serious effects over those habitats and native species. The LIFE LimnoPirineus Project has as the main goal the improvement of these aquatic systems and the species that live there. The elimination and management of Phoxinus sp. has been done to achieve this objective, from which the samples have been obtained to carry out this study.
The investigation done is based on the analysis of the growth and demography from the observation of otoliths, calcified bone structures located in the internal ear. Their visualization with binocular loupe allows the age determination of each individual, which will be related with the corresponding forkal length by a mathematical model named Von Bertalanffy. From this subsample used in the growth study and some estimation per proportions, the ages of each individual from samples not processed in the laboratory have been determined. With that, the analysis of the demography of each lake with the observation of the corresponding survival profiles and the instantaneous rate of mortality could have been done.
Results show meaningful differences in the studied populations, both for growth and demography, being each lake’s limnological characteristics and morphometry the factors that can affect those divergences, also taking into account the possible presence of different species of Phoxinus sp. in all the lakes. In the case of Closell Lake, these growth and mortality rates have been close related to the fact that this population has been already exploited and has suffered a previous treatment. Concluding, this gesture has had a great success and has achieved the considerably reduction of the population
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Ciprínids -- Pirineu català
Animals invasors -- Pirineu català
Biologia de poblacions
Introduced organisms -- Catalan Pyrenees
Cyprinidae -- Catalan Pyrenees
Population biology
Biological invasions -- Catalan Pyrenees
Invasions biològiques -- Pirineu català
Biología
Creixement i demografia de l’espècie invasora Phoxinus sp. en estanys d’alta muntanya dels Pirineus
info:eu-repo/semantics/bachelorThesis
east=0.9477269000000206; north=42.54797459999999; name=Estany de Dellui
east=1.2946713999999702; north=42.6827512; name=Estany Closell
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oai:dugi-doc.udg.edu:10256/112532015-10-14T00:01:25Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Sancho Badell, Sílvia
Ferrer Carré, Clara
Universitat de Girona. Facultat de Ciències
2015-10-13T10:26:32Z
2015-10-13T10:26:32Z
2015-06
http://hdl.handle.net/10256/11253
Preimplantation genetic diagnosis (PGD) is a technique associated with assisted reproduction
(AR) which is the analysis of genetic material from pre-embryo in order to determine if it is free
of genetic alterations and to transfer it to the uterus of the mother enabling the seed of a healthy
baby.
To do this project it has done a thorough search, searching many keywords in databases,
bibliographic and webgraphy sources and consulting specialists in medical field. To complement
the theoretical knowledge acquired stays were performed with Girofiv and Reprogenetics
groups.
PGD is a technique basically indicated in couples who have sex-linked diseases, monogenic
diseases, the search of histocompatible siblings, in numerical and structural chromosomal
abnormalities, in women who have had recurrent abortions or in patients over 35 years where
the doctor can suspect the presence of genetic alterations in oocytes. To analyse these situations
is usually provided genetic counselling by specialists where all possibilities available to patients
are determined. In the PGD it is necessary to obtain oocytes, which are extracted from ovarian
puncture with prior hormonal stimulation. Mature oocytes (either from the same patient or
donor, which can be cryopreserved) are fertilized in vitro or by intracytoplasmic injection to get
the pre-embryos, which are selected to extract one or more cells from a biopsy polar body,
blastomere or trophectoderm. The extracted DNA from the cells is analysed by three techniques:
PCR, FISH and CGH array.
The legislation of this technique is highly influenced by ethical and religious issues. Spain is one
of the countries of the European Union and the world that most uses the PGD techniques of RA,
having a fairly permissive legislation, always validated by specialized committee. Other
European countries have more or less restrictive legislation regarding this technique. Outside
Europe, the developed countries which use the DGP usually do not have separate legislation for
this technique, and they often regulate it by commissions or from what determines their
religion
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Biologia (TFG)
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http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Diagnòstic genètic preimplantacional
Reproducció humana assistida
Preimplantation genetic diagnosis
Human reproductive technology
Biología
Revisió de les tècniques aplicables al diagnòstic genètic preimplantacional (DGP)
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oai:dugi-doc.udg.edu:10256/112552015-11-10T14:00:10Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Funosas Planas, Gerard
Universitat de Girona. Facultat de Ciències
2015-10-13T10:41:25Z
2015-10-13T10:41:25Z
2015-06
http://hdl.handle.net/10256/11255
The large stretch of power lines that are found in our landscapes has an effect on the environment they surround, and one of their most important impacts are the accidents with the bird community, either by electrocution or collision. The most affected group are the birds of prey, given that they frequently use the electric posts for perching and, in general, they are species with reduced populations and with an improvable conservation status.
This project aims to analyse these problems in the mountain range of Ardenya or Cadiretes, located in the Catalan coast, between the regions of la Selva, el Gironès and el Baix Empordà. Its geological singularity and its natural heritage have included that area as a PEIN, and made the region a favourable habitat for some endangered raptor species associated with rock massifs, as the great owl (Bubo bubo), the peregrine falcon (Falco peregrinus) or the bonelli’s eagle (Aquila fasciata). Due to that fact, during the months of February and March, about 40 km of the high power lines were sampled, corresponding to the 70% of their total expanse. Electrocution evidences (as groups of feathers, corpses or rests of carcasses) have been searched under the utility pole, which have been geo located, photographed and catalogued according to their potential danger. This classification has been done taking into account an assembly of variables, related to the tower configuration and also to the environment their surround, following the methodology done by Tintó et al. (2010).
We’ve found few electrocution evidences, with a sample unit of 4, for all the 357 utility poles sampled. Nevertheless, the result value is considered a lot lower than the actual would be, and its explanation would be allocated by the carnivorous mammals which move away the bird corpses. This statement is reinforced by the excrements of those found regularly on the posts bases, which demonstrate their recurrence under the power lines. At the same time, the virtually totality of high power lines have been placed in a map, and some corrective measures have been designed for the 9 most dangerous utility poles, from a conservative insight. Two specific censuses for studying the raptors community have been done, which considered together with the observations brought by other bibliographic sources, have given an 18 species list of birds of prey that inhabit in the mountain range or that may frequent it
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Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Rapinyaires -- Catalunya -- Cadiretes, Massís de
Línies elèctriques -- Aspectes ambientals -- Catalunya -- Cadiretes, Massís de
Birds of prey -- Catalonia -- Cadiretes, Massís de
Electric lines -- Environmental aspects -- Catalonia -- Cadiretes, Massís de
Biología
Efectes de les infraestructures elèctriques sobre els ocells a l’EIN de Ardenya-Cadiretes
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112562015-10-14T00:01:28Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Furriols Espona, Marc
Mineo, Alessandro
Sanz Ball-llosera, Núria
Furriols Espinosa, Enric
Universitat de Girona. Facultat de Ciències
2015-10-13T10:52:48Z
2015-10-13T10:52:48Z
2015-06
http://hdl.handle.net/10256/11256
Identifying serine proteases involved in the localized
activation of the tyrosine kinase Torso receptor in the Drosophila melanogaster embryo.
Abstract: This Degree Final Project focuses in genetic experiment using Drosophila
melanogaster as a model system. More specifically, the project is framed within one of
the aims of the laboratory lead by Dr. Jordi Casanova in the Institut de Biologia
Molecular de Barcelona (IBMB) which aims at identifying the mechanisms involved in
the localized activation of the Torso receptor in the Drosophila embryo pulse. More
specifically, in identifying a serine protease that is involved in the activation through
proteolytic processing of an essential ligand towards the activation of the receptor,
called Trunk. The Torso receptor is the responsible for the formation of the terminal
structures of the embryo.
In Drosophila there are over 200 serine protease and to be able to identify the protease
involved in the processing of this ligand, the phenotype produced by the lack of
function of each of the different serine proteases inactivating their function using the
interference RNA technique (RNAi). By doing so, inferring in the function of a specific
protease and, from the germ phenotype observed, generic interactions can be established.
In order to do so, an experimental method was designed so as to test the inactivation of
every protease.
In order to check that the screening strategy was really the most convenient one, and so
to check that the experimental design was valid and able to spot a protease involved in
the terminal system, RNAi from well-known elements in the route of the Torso receptor
were checked. The result showed that this strategy worked.
Nevertheless, no protease has been found involved in the Trunk processing so far, since
no germ phenotype related to the lack of terminal structures has been observed
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Drosòfila -- Genètica
Drosophila GEC -- Genetics
Biología
Identificació de serina proteases implicades en l’activació localitzada del receptor Torso tirosina cinasa a l’embrió de Drosophila melanogaster
info:eu-repo/semantics/bachelorThesis
east=2.118516399999976; north=41.38256579999999; name=Institut de Biologia Molecular de Barcelona
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oai:dugi-doc.udg.edu:10256/112572015-11-10T14:00:39Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Fusellas Fullà, Marc
Universitat de Girona. Facultat de Ciències
2015-10-13T10:58:25Z
2015-10-13T10:58:25Z
2015-06
http://hdl.handle.net/10256/11257
Invasive species cause economic and ecological losses. In this study we attempt to
obtain biological and ecological information about two invasive species, Pyracantha coccinea,
and Lonicera japonica which produce fleshy fruits and interact ecologically with frugivorous
fauna in order to be dispersed by endozoochory, or in the antagonist ecological relationship of
seed depredation. Here we determine the effect of the frugivores over the invasion potential
of these two species, and we assess how to improve invasion species control management in
the Puda Forest in Banyoles.
In our study we excluded any branches covered by mosquito or insect nets, which would have
made it impossible for the fruits on the branch to be consumed, and we used the net-free
branches (our control group) to determine the fruit loss (fruit removal) from the branches.
Additionally, we carried out a census of the bird and micromammal communities, in order to
determine what kind of frugivores there were in the study zone i.e. either mutualist or
antagonist. The results conclude that because a huge percentage of fruits are consumed by
frugivores, they have a significant effect on the dispersion and depredation of seeds in the
both species. If frugivores did not interact with the plants, the fruits would stay on the
branches for almost 8 months even after reaching their peak ripeness. However, we have to
take in account that in the census we find legitimate frugivores, pulp eaters and seed
depredators; therefore not every fruit that is consumed benefits that plant itself.
Finally, the study proposes two different types of management programmes. The first one is
for the Puda Forest, and involves eliminating the exotic plants which are to be found on the
edge of the forest, in other words those which have taken over the greatest area and
consequently have more opportunities to reproduce and disperse their seeds. Conversely,
those found in the middle of the forest will grow slowly and will die thanks to the shade and
the competition the adult ashes provide.
The second management strategy is to establish a programme to create and monitor an
invasive-plant-free forest (like the Puda Forest) in an abandoned crop field
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Plantes invasores -- Control -- Catalunya -- Banyoles
Invasions biològiques -- Control -- Catalunya -- Banyoles
Fauna forestal -- Catalunya -- Banyoles
Invasive plants -- Control -- Catalonia -- Banyoles
Biological invasions -- Control -- Catalonia -- Banyoles
Forest fauna -- Catalonia -- Banyoles
Biología
Efecte de la fauna frugívora sobre el poder invasiu de les espècies vegetals exòtiques de fruit carnós
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112582015-10-14T00:01:26Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Roldán Borassi, María Inés
García Soto, Arantxa
Universitat de Girona. Facultat de Ciències
2015-10-13T11:02:31Z
2015-10-13T11:02:31Z
2015-06
http://hdl.handle.net/10256/11258
During the last years, the population of Barbary macaques has suffered a drastic decline. The destruction of their habitat and the illegal pet trade are the main threat to this species. Over time, the owners are aware of the difficulties involved in the care of wild animals such as the Barbary Macaque, which in most cases leads to neglect and/or isolation of these animals by leaving them in small cages. Because of their illegal status, these individuals are seized by authorities and distributed in shelters and sanctuaries such as Mona Foundation. Abnormal and/or stereotyped behaviors are commonly observed in the evaluation of species of primates in captivity and the Barbary Macaques are not an exception. The study was carried out in the Primate Recovery Center of Mona Foundation, which, since 2000, has been dedicated to the rescue, recovery, rehabilitation and resocialization of primates. This research project is to improve the welfare of a group of four Barbary macaques with an environmental enrichment plan based on the structural change of their enclosure. The research focuses on the effects of this structural enrichment by studying the behavior and the use of space of this group of macaques during their rehabilitation and resocialization process at Mona Foundation. The group of macaques has presented an improvement of their behavior after the treatment. The structural enrichment showed changes in the composition of the environment which determines the cognitive capacity of individuals allowing the expression of behaviors typical to their species. The structural changes have minimized the stress and the occurrence of stereotypic behaviors, promoting positive behaviors and therefore achieving the main objective, which is promoting and improving the welfare of the group
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Animals salvatges en captivitat
Primats -- Hàbits i conducta
Captive wild animals
Primates -- Behavior
Biología
Modificación estructural del recinto de un grupo de Macacos de Berbería (Macaca sylvanus) cautivos: uso del espacio y cambios en el comportamiento
info:eu-repo/semantics/bachelorThesis
east=2.816703999999959; north=41.898252; name=Fundació Mona
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oai:dugi-doc.udg.edu:10256/112592015-10-14T00:01:26Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vilar Sais, Lluís
Garrido Sanz, Lidia
Universitat de Girona. Facultat de Ciències
2015-10-13T11:09:03Z
2015-10-13T11:09:03Z
2015-06
http://hdl.handle.net/10256/11259
Simethis mattiazzi is an atlantic distribution plant which arrived to Catalonia during postglacial times of the Quaternary period. Lately, with the beginning of climatic warming and the setting-up of Mediterranean climate in Catalan lowlands, its distribution has been reduced and only has been kept at la Selva's plain, due to its submediterranean climate with Atlantic tendency. Nowadays, it can only be found in some parts of la Selva's depression, many of them threatened by human activity. Recently, the Generalitat de Catalunya has catalogued this taxon as a vulnerable species.
The main objectives of our study is to know the physiology and the conservation state of this taxon in Catalonia and identify the factors that benefit and disadvantage its propagation. For this reason, this study is divided into two large blocks.
1. The conservation status of the species in the plain of La Selva, information taken from:
The location, delineation and habitat characterization of all stocks of S. mattiazzi at La Selva's plain.
Scrutinizing the number of individuals and proportion of which are sterile, flowers and fruiting, to know the population's density and the capacity of the taxon to reproduce.
The effect of the diminishment in habitat quality, from the comparison of the number of individuals which are in areas of dense woodland compared to the number of individuals which grow in clearings and open areas.
2. Ex situ monitoring germination of seeds, information taken from:
The study of seeds' viability with the tetrazolium test, in order to know the dispersion capacity and maintenance of the species over time.
Monitoring of morphology and physiology during embryo development under different conditions of temperature, light and presence or absence of elaiosome, to find out which conditions must undergo to break seed dormancy and germinate.
The germination capacity of the species.
The first results will help us to get a first idea of the cycle of the species and, especially, the ecological status of the taxon in Catalonia. These data will help almost immediately to develop a management plan to maintain it and, if possible, to propagate the species in our country.
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Flora amenaçada -- Catalunya -- Selva
Flora -- Catalunya -- Selva
Endangered plants -- Catalonia -- Selva
Plants -- Catalonia -- Selva
Biología
Demografia i conservació de Simethis mattiazzi, espècie d'interès per a la flora vascular de Catalunya
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112602015-10-14T00:01:29Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Muñoz Frigola, Marta
Ibars Estiarte, Eva Irene
Universitat de Girona. Facultat de Ciències
2015-10-13T11:21:10Z
2015-10-13T11:21:10Z
2015-06
http://hdl.handle.net/10256/11260
The European anchovy (Engraulis encrasicolus) is one of the pelagic species with biggest commercial interest and at the same time, one of the most threatened by overfishing in the Mediterranean Sea. It is a species with external fecundation which releases thousands of eggs in each lay and presents a high mortality rate, so only a low percentage of eggs finally hatch. However, the anchovy lives in an environment with constant fluctuations and its reproductive strategy allows it to handle high population drops. Notwithstanding, overexploitation threshold in which the species is subdued nowadays staggers its individuals resilience and is able to affect Mediterranean populations subsistence in the long term.
In this context of threat in which anchovy is in the Mediterranean, it is important to also consider the possible impact of coccidian parasitism in males’ gonads. Hosts’ infection can worsen the health status of the Mediterranean stocks. Infected males can suffer parasitism consequences in their testicular development, the creation and the release of sperm. Moreover and besides the effects that coccidia can directly have on the species reproduction, this project also studies if these parasites compromise hosts health.
In the first place, it studies which kind of coccidia parasite male anchovy’s gonads. After that, this project analyses the parasites prevalence on the basis of different variables such as the months of the year, the testicular development stages or the amount of mesenteric fat of the individuals. Finally it studies the relationship between the presence or the absence of parasites and other factors like gonadosomatic index or the physiological condition factor, through which it is possible to infer parasitism effects on the reproductive potential and the health condition of the infected individuals.
On the whole, the study analyses coccidian parasitism effects on a population which is also threatened by the fishing pressure at which it is subdued
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Attribution-NonCommercial-NoDerivs 3.0 Spain
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Anxoves -- Paràsits
Anxoves -- Reproducció
Anchovies -- Parasites
Anchovies -- Reproduction
Biología
Parasitosi per coccidis en mascles d’anxova
info:eu-repo/semantics/bachelorThesis
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Menció i Domingo, Anna
Bartumeus Ferré, Frederic
Lara Martínez, Eva
Universitat de Girona. Facultat de Ciències
2015-10-13T11:29:54Z
2015-10-13T11:29:54Z
2015-06
http://hdl.handle.net/10256/11261
The tiger mosquito (Aedes albopictus) is a species native to Southeast Asia and it was first detected in Catalonia in 2004 and currently has colonized all over the peninsula. In addition, this species of mosquito has the ability to act as a vector of several diseases, such as malaria, dengue and Chikungunya. Thus, it is an invasive species of ecology and urban habitats, which hinders its eradication and enhances the capacity to transmit diseases to the human population.
ICREA Movement Ecology Laboratory in collaboration with the Centre for Advanced Studies of Blanes CEAB-CSIC and the Centre for Ecological Research and Forestry Applications (CREAF) have developed a new application called Tigatrapp in which citizens can send pictures of Tiger Mosquito or breeding sites from their smart phone and this information is automatically shown in an interactive map on the website. This allows us to establish a closer link between the participants and the scientific world, because citizens have the possibility to cooperate in locating tiger mosquito in order to model the dispersion, and provide new insights into scientific research.
This project is based on statistical analysis to determine the behaviours that users follow when they have downloaded the application, also with the complementation of different methods of analysis to classify participants into groups of similar profiles. The aim of this study is to understand the trends of participation and determine whether the citizen science is really a useful tool for active control of mosquitoes providing continuous and important data throughout this project called AtrapaelTigre.com. To fulfil this, it is essential to determine possible weaknesses and possible issues to improve so as to encourage the citizens’ participation.
The study is based on two fundamental databases which have been extracted from the application server that contains data from 13rd of June, which corresponds to the day on which the application is available, until 1st of December 2014. All of the reports and users are analyzed through statistical programs in order to achieve objectives
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Mosquit tigre -- Recursos electrònics en xarxa -- Participació ciutadana
Aedes albopictus -- Computer network resources -- Citizen participation
Biología
Estudi de les tendències de participació i els perfils d’usuaris dins del projecte AtrapaelTigre.com sobre el monitoratge del mosquit tigre (Aedes albopictus)
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112622015-10-14T00:01:27Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Sánchez Navarro, Juan Manuel
Mateo Sendino, Eva
Universitat de Girona. Facultat de Ciències
2015-10-13T11:35:32Z
2015-10-13T11:35:32Z
2015-06
http://hdl.handle.net/10256/11262
Cerebral ischemia or stroke is a cerebrovascular disease caused by a reduction of blood flow in a certain area of the brain. It is the leading case of disability in adults and the second leading cause of death worldwide (Jin et al., 2010). The blood failure in the brain causes changes in the neuronal metabolism, such as massive entry of intra-cellular calcium, caused by a lack of oxygen and nutrients (Guevara et al., 2004). The increase of calcium intra-cytosolic enables the release of several neurotransmitters such as glutamate and nitric oxide. A high concentration of these neurotransmitters has a toxic effect on the cell, because of the stimulation of calcium entry and the release of free radicals (RLO) which has the capability to degrade proteins, nucleic acids and phospholipids (Guevara et al., 2004).
Time is an essential factor in order to prevent brain damage, if a fast blood reflux happens time can prevent cell death. The recombinant plasminogenic tissue activator (rt-PA) is the only approved treatment for combating cerebral ischemia, although it has an important limitation: it is only effective up to a maximum of three hours from the beginning of the stroke (Fernandez-Gomez et al., 2008).
The main goal of this study is to analyse and quantify free amino acids presented in the plasma of patients who have suffered an ischemic stroke and have been supplied with the rt-PA treatment. The aminoacids’ test is performed by a derivatization of the samples, followed by a high resolution chromatography determination in a reverse phase, with a Gemini NX-C18 column (250 x 4.4 mm) with 5μm in size of Particle and UV detection. A buffered acetate/phosphate solution, adjusted to pH 5.38, and a B acetonitrile (60%) organic solution has been used as a mobile phase.
In other studies (Tcherkas et al., 2001), non treated rt-PA patients show a gradual increase on glutamate, that can reach to 15 days. The results obtained by this study prove that the rt-PA treatment has a significant effect on certain amino acids, such as glutamate, because it keeps the concentration at a stable level. These results suggest that rt-PA not only acts dissolving formed clots but also, in a molecular level, acts evading the level increase of neurotransmitters (glutamat)
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Teràpia trombolítica
Isquèmia cerebral -- Tractament
Cerebral ischemia --Treatment
Thrombolytic therapy
Biología
Determinació d’aminoàcids lliures en plasma de pacients amb ictus amb tractament trombolític
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/112632015-11-17T08:20:06Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Colomer, Jordi
Martín Sintes, Daniel
Meca Jimenez, Miguel Angel
Universitat de Girona. Facultat de Ciències
2015-10-13T11:43:28Z
2015-10-13T11:43:28Z
2015-06
http://hdl.handle.net/10256/11263
Oxydromus humesi is a strict bivalve endobiont, probably a parasite, living in
association with Tellina nymphalis in north of Pointe-Noire (Congo mangrove swamps, West
Africa) and Scrobicularia plana (intertidal) and Psammotreta cumana (subtidal) in Cádiz Bay
salt marshes (southern Atlantic coast of Iberian Peninsula). The symbiont shows a regular
distribution (one, exceptionally two or three symbionts per host, being one male, one female
and one small worm) with intraspecific competition and complex host-entering behaviour
observed in experimental conditions. This work aims to study the population dynamics of the
above-mentioned species hosted by S. plana in Cádiz Bay to increase knowledge on the
biology of O. humesi and to contribute to the growing knowledge on symbiotic polychaetes.
The population of the symbiont in the intertidal area of Rio San Pedro (Cádiz Bay) seems to
reproduce actively through all the year, with a higher number of ripe females in spring and,
particularly, in summer. Although S. plana is very abundant in the studied area, with a large
amount of specimens having the size most preferred by the symbiont (i.e., from >26 mm to
about 36 mm long), the prevalence tends to be very low (usually <5% and reaching up to
~20%). This fact, together with the presence of a highly infested population (>85% in the
specimens longer than 20 mm) of P. cumana in Río San Pedro mouth, lead us to discuss on
the environmental requirements and host-specificity of O. humesi. We suggest that the studied
intertidal could be at the limit of the ecological distribution of this symbiont in the Cádiz Bay
region. The symbiotic hesionid seems to show a seasonal pattern of prevalence, which may be
related with its reproduction (the periods with more ripe females tended to coincide with those
of lower prevalence). Taking into account these data, we propose that males undertake
reproductive migrations probably to improve the possibilities of fertilization, whilst the
females stay throughout the whole life inside the host. The life-cycle of O. humesi remains
unknown since the species’ discovery in the coasts of the Republic of Congo. However, our
data allow us to suggest that its life span may be of one year, with the adults dying after
reproducing
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Bivalves -- Andalusia -- Cadis
Poliquets -- Andalusia -- Cadis
Simbiosi
Biologia de poblacions -- Andalusia -- Cadis
Polychaeta -- Andalusia -- Cadiz
Symbiosis
Population biology -- Andalusia -- Cadiz
Biología
Study of the population of the symbiotic polychaete Oxydromus humesi hosted by the bivalve Scrobicularia plana in Cádiz Bay
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/112642015-11-10T14:00:28Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Sargatal, Jordi
Pagès Jaén, Pau
Universitat de Girona. Facultat de Ciències
2015-10-13T12:03:31Z
2015-10-13T12:03:31Z
2015-06
http://hdl.handle.net/10256/11264
Following the trend of many studies that move motivated by wanting to better understand
the causes of the globally decline suffered by the common barn owl (Tyto alba), this study
aims to know the situation of the population of barn owl in the Parc Natural dels Aiguamolls
Empordà (PNAE). This study takes place within the Projecte Òliba, which began in the PNAE in
order to evaluate and improve the situation of the barn owl population in the area. After two
years since the start of the project, the aim of this study is to review the actions that have
been done and determine which is the current status of the population of barn owls in PNAE. I
analyzed 75 human constructions, 23 nest boxes has been tracking and I have quantified the
breeding couples and its characteristics. I have also been able to compare data from previous
years with the data obtained with the current actions.
I observed a decrease of barn owls in buildings where there are clear traces of their presence
in the past (24% of the points analyzed) and I checked that sometimes this is due to actions of
the owners. In addition, this revision has also determined that there is a minimum of 4
couples breeding in the park which are not free of risks and dangers, fact that puts the
population in a fragile situation. Despite this, it has been checked that nest boxes may be an
alternative to the loss of roost and nest sites, considering its collocation in places without
unwanted species such as the pigeon (Columba livia domesticus).
However, it is necessary to take into account that some data were insufficient to make a good
assessment of the ecological status of the population. For example, the average of eggs per
pair and success of the young barn owls could be far from reality because of sample size (N =
4). Therefore, this study offers a base and some actions from which next studies should
continue working
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Òliba -- Catalunya -- Parc Natural dels Aiguamolls de l'Empordà
Barn owl -- Catalonia -- Parc Natural dels Aiguamolls de l'Empordà
Biología
Avaluació de la situació de la població d'òlibes (Tyto alba) al Parc Natural del Aiguamolls de l'Empordà: estat de l'hàbitat, ocupació de caixes niu i reproducció
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oai:dugi-doc.udg.edu:10256/112652015-10-14T00:01:36Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Peñarrubia Lozano, Luis
Palacios Marco, Mariona
Universitat de Girona. Facultat de Ciències
2015-10-13T12:08:19Z
2015-10-13T12:08:19Z
2015-06
http://hdl.handle.net/10256/11265
Zebra mussel (Dreissena polymorpha, Pallas, 1771) is a bivalve mollusc species native from the Caspian and Black seas region. Since XIX century has expanded to central Europe, and it established in the Iberian Peninsula in 2001. The presence of D. polymorpha causes ecological impacts, affecting biodiversity, and economic impacts, blocking water-conductor infrastructures.
In order to control and manage the infestation, different control and management plans have been established. Some of these organizations are Agencia Catalana de l’Aigua (ACA), Confederacion Hidrogràfica de l’Ebre (CHE), and Agencia Basca de l’Aigua (URA). These monitoring plans are carried out by observing larvae through a microscope, a technique with low specificity and sensitivity that can generate false positives. For this reason the molecular methods can be an alternative more reliable that could fix the problems like un-specificity and low sensitivity.
Using gene histone H2B and Real-Time PCR, it is expected to be optimized a method to determine the infestation grade from environmental filtered-water samples. For do this, it’s necessary to optimize the previous steps, like DNA isolation and PCR amplification. The samples used in this project belong to different locations from Catalonia sampled in two seasons.
Results obtained show that D. polymorpha DNA is present in all analysed locations. ACA has only confirmed the presence in one of them (La Baells Reservoir), suggesting the better molecular techniques specificity and sensitivity than traditional visual methods. We also have seen that in winter there are more D. polymorpha DNA prevalence in the water column than in summer, according with the reproduction period.
This molecular analysis can be used like routine tool into the D. polymorpha control and management plans. Because its quickness and its great D. polymorpha DNA detection, the prevention efforts could be focussed to the locations with risk of infestation
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Musclos d’aigua dolça -- Genètica
Animals invasors
Invasions biològiques
Freshwater mussels -- Genetics
Introduced organisms
Biological invasions
Biología
Detecció genètica mitjançant PCR a temps real de la presència de l’espècie invasora musclo zebrat (Dreissena polymorpha)
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/112712015-10-15T00:00:52Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vera Rodríguez, Manuel
González Pérez, Josefa
Rovira Castellà, Quirze
Universitat de Girona. Facultat de Ciències
2015-10-14T07:07:12Z
2015-10-14T07:07:12Z
2015-06
http://hdl.handle.net/10256/11271
Transposable elements (TEs) are mobile DNA sequences that have the ability to jump
from one location of the genome to another and create copies of them during the
process. Although their activity can create mostly harmful mutation, it also represent an
important source of genetic variation that have been shown to rewire and fine-tune
regulatory networks. Stress response mechanisms are highly conserved across
organisms and a major target of natural selection. Despite the latest advances in
evolutionary biology, adaptation process remains poorly understood. Therefore,
studying stress pathways provide a great opportunity to study how adaptive processes
occur. In this study we aim to identify genome-wide putatively adaptive TEs that are
candidates to modulate stress response by the addition of stress response elements
(SREs) in the promoter of Drosophila melanogaster genes. We combine bioinformatics
and population genetics approaches to discard SREs found by chance and select TEs
having SREs that are more likely to be involved in stress response. We found 3,381 TEs
out of 5,416 annotated TEs that contain at least one SRE. Because only TEs inserted in
the promoter region are likely to have a biological impact in the gene modulation we
reduce our dataset to 406 TEs. Moreover, we select TEs present at high frequency in the
populations since they are more likely to be adaptive. Finally, our curated dataset
contain 108 TEs with at least one SRE, inserted at a promoter region of a gene and at
high frequency in at least one of the 8 worldwide studied populations. Out of this list we
in silico validate 18 TEs thanks to modENCODE project and other chromatin immuneprecipitation
studies. Additionally, we analyzed the promoter of the 127 genes nearby
the 108 TEs. The function of 22 out of this list was related to stress by genome wide
association studies and gene ontology term annotations. Eighty-five TEs add preexisting
or novel SREs while other 26 introduce SREs to the regions without any previous
SREs. Having several SREs make the promoter region and/or the TE more likely to
have an impact on the gene expression. Seventy-three TEs out of the 108 TEs contain
more than one SRE and hence are more likely to be involved in stress response
regulation. Our final candidate list is the starting point to functionally analyze the role
of these putatively adaptive TEs in stress response
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Drosòfila melanogaster -- Genètica
Biologia de poblacions
Drosophila melanogaster -- Genetics
Population biology
Biología
The Role of transposable elements in regulatory networks in response to stress
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/112722015-10-15T00:00:58Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Molinas de Ferrer, Maria Lluïsa
Boher Genís, Pau
Sánchez Peláez, Ana
Universitat de Girona. Facultat de Ciències
2015-10-14T07:11:13Z
2015-10-14T07:11:13Z
2015-06
http://hdl.handle.net/10256/11272
The presented thesis is related to the cork formation. The objective is to determine by quantitative PCR (qPCR) the expression level of a group of candidate genes for a better understanding about the possible role of these genes and the molecular basis for the cork formation.
For these genes has been analyzed its specificity phellem through the comparison between secondary tissue producer suberin (phellem/cork) and non-secondary tissue producer suberin (xylem/wood) in samples of cork oak obtained on the period of maximum growth of cork; the differential expression between phellem of cork oak (Quercus suber) and holm oak (Quercus ilex), to understand the relevance of these selected genes in the formation process since the cork oak produces many more layers of cells of phellem for growth station than the holm oak; and the expression pattern of these genes during seasonal growth of cork in phellem samples obtained along the period of growth, to understand through the seasonal variation in their expression as they are involved in the formation of cork and comparing the expression pattern of other genes previously studied.
For some of the candidate genes selected has been validated an specificity in suberized tissues (phellem); it checked that the candidate genes of phellem together are expressed although more in cork oak, and thus validated the results of the study by massive sequencing of the transcriptome comparison of cork oak and holm oak; and the seasonal pattern has been determined for all the candidate genes. The results of the seasonal pattern allowed us to see that the structural genes related to lipid synthesis and deposition of suberin, are following a seasonal pattern typical of suberin genes and that hormone receptors are more active phase of maximum growth of cork; and about the regulatory genes, RIK possibly is a negative regulator of the process of cork formation, WOX4 could play an important role related to the maintenance of undifferentiated cork cambium in to the start of the growing season during in the april, and AP1 is more active on the maximum formation and development of cork period
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Alzina -- Genètica molecular
Surera -- Genètica molecular
Holm oak -- Molecular genetics
Cork-tree -- Molecular genetics
Biología
Determinació de l’expressió diferencial de gens al llarg del creixement estaciónal del suro
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/118302016-01-12T01:00:43Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Sancho Badell, Sílvia
Carrasco Vila, Marina
Universitat de Girona. Facultat de Ciències
2016-01-11T15:20:30Z
2016-01-11T15:20:30Z
2015-09
http://hdl.handle.net/10256/11830
Sperm cryopreservation is a technique integrated in the animal reproductive
biotechnology that allows the preservation of male gametes at low temperatures,
particularly at -196ºC, for long periods of time in order to maintain their fertilizing capacity.
The technique is used in different animal species for various purposes, including the
reproductive, the preservation of genetic material, the easier international trade, etc. The
work methodology has consisted of a literature review conducted in several databases by
entering keywords to search for different publications such as theses, scientific articles,
review articles, all related to sperm cryopreservation. In this work has been used as a
model the boar sperm, which presents a greater susceptibility to cryopreservation due to
the high content of polyunsaturated fatty acids and the low cholesterol presence in its
membrane. From 2006 until today, the use of boar semen cryopreserved for the practice
of artificial insemination (AI) represents only 1% of the inseminations carried out, this is
due to the fertility rate and litter size using frozen semen is much lower than that obtained
when using fresh cooled semen; with a 20-30% of difference in the fertility rate and 2-3
piglets less per litter. In recent years, several studies have been conducted to optimize the
protocol for cryopreservation of semen of this species in order to improve sperm survival
and the fertilizing capacity of sperm thawed. In this work has been reviewed some of the
improvements to the cryopreservation protocol tested until now as: modifications of the
technique itself and adding cholesterol, antioxidants or seminal plasma in freezing
extenders. Despite the improvements achieved over the years, it is necessary to continue
testing with new substances and different concentrations of the substances studied so
far, in order to optimize and monetize the technique of cryopreservation of boar sperm to
be of interest within in the sector
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Porcs -- Espermatozoides -- Investigació
Porcs -- Inseminació artificial
Crioconservació d'òrgans, teixits, etc.
Porcs -- Recursos germoplasmàtics -- Crioconservació
Swine -- Artificial insemination
Swine -- Spermatozoa -- Research
Cryopreservation of organs, tissues, etc.
Swine -- Germplasm resources -- Cryopreservation
Biología
Revisió de la tècnica de criopreservació espermàtica i estudi de la seva optimització en biotecnologia de la reproducció porcina
info:eu-repo/semantics/bachelorThesis
NO
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Romaní i Cornet, Anna M.
Campos i Llach, Miquel
Coma Leal, Núria
Universitat de Girona. Facultat de Ciències
2016-01-11T15:24:57Z
2016-01-11T15:24:57Z
2015-09
http://hdl.handle.net/10256/11831
Freshwater mussels (Mollusca, Bivalvia, Unionoidea) are one of the most threatened animal groups in Catalonia. Therefore, artificial culture establishes itself as a very important tool for their conservation. LIFE Potamo Fauna works for the conservation of freshwater mussels in Girona’s rivers from a captive breeding program carried out in three steps: obtain glochidia from breeding females, infestation of host fish and breeding and fattening of juveniles.
The aim of this paper is to understand what is the best rearing and feeding strategy of juveniles mussels Unio mancus Lamarck, 1819 in the laboratory from growth and survival values of two differents breeding methods.
In the first experiment juvenile were feed with three different diets (based on combinations of algae and detritus) and five different food doses and put inside glass containers with 500 ml of water during a period of 40 days. In the first 15 days, results shown a major growth of juveniles in response to a diet based on different algae: Nannochloropsis gaditana, Tetraselmis chuii and Phaeodactylum tricornutum. However, high mortality due to uncontrollable external factors, make impossible to know if this results occur along experimental days. Feeding juveniles with detritus, however, decrease growth values.
In the second experiment we breed juveniles in a closed aquaculture system with three different treatments based on water recirculating speed (high, low or null) and with the same kind of diet. Treatments with high and low recirculation shown major mortalities in 40 days due to the effect of both the recirculation of water and the aeration.
We determined that the best way to breed juveniles of Unio mancus indoor is an aquacultural system without water recirculation, where juvenile mussels showed the most favorable growth (1.432,17 +/- 136,6μm) and survival values (57,5 +/- 15,26%) at 60 days.
It can be concluded that the most effective method for breeding juveniles is the one with the best algae formulation for the species studied and excellent qualities of water and culture environment
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Nàiades (Mol•luscos) -- Reproducció
Nàiades (Mol•luscos) -- Alimentació
Freshwater mussels -- Reproduction
Freshwater mussels -- Feeding and feeds
Biología
Experimentació en el cultiu de juvenils de nàiade allargada (Unio mancus Lamarck 1819) en condicions de laboratori
info:eu-repo/semantics/bachelorThesis
NO
ORIGINAL
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oai:dugi-doc.udg.edu:10256/118322016-01-12T01:00:47Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Brusi i Belmonte, David
Pou i Rovira, Quim
Cros Chertó, Cristina
Universitat de Girona. Facultat de Ciències
2016-01-11T15:33:45Z
2016-01-11T15:33:45Z
2015-09
http://hdl.handle.net/10256/11832
Unio mancus and Unio ravoisieri are two of the six species of naiads (freshwater mussels) that inhabit catalan aquatic ecosystems. Both are included in the project LIFE Potamia Fauna, which one of its objectives is to improve the population centers that exist in the rivers Ter and Fluvià since it’s seen a sharp decline in recent years. It is very important to study the naiads, because as they are filtering organisms have an important role in aquatic ecosystems.
These two species are very similar in morphology and behavior, and it is known that live half buried in the sediment, usually of riverbanks and even sometimes they are hidden among the roots of trees. Actually, however, little is known about its habitat. That is why in this study it’s wanted to delve a little deeper and find out if there is some relationship between some environmental variable and the presence of naiads. 3
To do the research, ita was carried out in different sections of rivers Llémena and Ser, and som ditches of Banyoles and Brugent. Each part is divided into sections, where is sampled more data about naiads’s general inhabitat. Within each section, were also randomly chosen small areas of a meter in length, which has been a more specific micro-habitat of the naiads. With the data, we calculated the relative density of naiads and their average length in each case. Values taken into account when performing statistical calculations.
The final results concluded that there is indeed a relationship between the density of naiads in a particular habitat and environmental variables. The following variables are a clear example of positive correlation in the rivers: a sediment composed of silt and clay, the presence of roots in the margins, the maximum depth and average depth. Verticality and cave are two variables whose together with the rest described play a key role when forming the micro-habitat more suitable for these two species of bivalves
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Nàiades (Mol•luscos) -- Hàbitat
Ecologia fluvial -- Catalunya -- Girona
Freshwater mussels -- Habitat
Stream ecology -- Catalonia -- Girona
Biología
L’Hàbitat de nàiades en el marc del projecte LIFE Potamo Fauna
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/118332016-01-12T01:00:48Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Sargatal, Jordi
Fernández García, Cristina
Universitat de Girona. Facultat de Ciències
2016-01-11T15:38:26Z
2016-01-11T15:38:26Z
2015-09
http://hdl.handle.net/10256/11833
Last years, European roller Coracias garrulus has undergone a moderately rapid decline across its global range and if this negative trend persists, the status of this species is likely to become Vulnerable in the IUCN Red List. Understanding the strong link between breeding success and bird conservation, this study aims to evaluate possible correlations between nest-site and landscape characteristics and habitat selection and breeding success in this species. This study has been conduct within the natural reserve “Els Aiguamolls de l’Empordà” (PNAE) where the last years European roller population has been stable up to now. Thirtyfive nest-boxes and three Green Woodpecker Picus viridis holes were analysed with field data collection on several nest-site and landscape characteristics. In addition, the establishment of breeding pairs and those with breeding success were quantified. A comparison to 2014 field database shows evidences of a worrying decreasing trend of European roller breeding pairs within the natural reserve indicating the deterioration and loss of suitable nest-boxes as the main factor. As a consequence, inter and intraspecific competition has been registered and also extremely rare data was collected, a nest-box has probably been parasitized by the European cuckoo Cuculus canorus. When compared to unoccupied nest-boxes, occupied nest-boxes were at a lower height (6,89 1,12 m), mostly oriented east, south and southeast therefore were in warmer nest-box microclimates and mostly had circular entrance holes. Although the foraging habitat selection data has been scarce, interesting data such as flight average distance and frequency during the foraging period for chicks has been collected. In this period flights were normally about 200-300 m long but quite fast (mostly about 3-5 min). The present study has also provided a detailed behavioural description during the nest foraging provisioning that highlight the importance of the hedgerows and riparian forest In the surroundings of the nest to allow the birds to perch and detect their prey. Finally, the results have led to the suggestion of a viable framework of conservation measures for the European roller in the natural reserve (PNAE)
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Gaig blau -- Catalunya -- Parc Natural dels Aiguamolls de l'Empordà
European roller -- Catalonia -- Parc Natural dels Aiguamolls de l'Empordà
Biología
Anàlisi poblacional i reproducció del gaig blau (Coracias garrulus) al Parc Natural dels Aiguamolls de l’Empordà
info:eu-repo/semantics/bachelorThesis
east=3.092050699999959; north=42.2247746; name=Aiguamolls de l'Empordà
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oai:dugi-doc.udg.edu:10256/118342021-06-14T08:33:41Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Verdaguer Murlà, Dolors
Vilajeliu i Serra, Marià
Garcia Celada, Irene Carla
Universitat de Girona. Facultat de Ciències
2016-01-11T15:43:48Z
2016-01-11T15:43:48Z
2015-09
http://hdl.handle.net/10256/11834
Lately we had observed the arrival of Dasineura mali, a small dipter which affects the development of
the young or grafted apple-tree. This study pretends, in one hand, to know and dig deeply on the biology
of the species and, in the other hand, to determine the ideal moment to apply different systems of
control and the evaluation of the efficiency in several active ingredients used in the phytosanitary
treatments. We have established and subdivided plots for the proposed thesis and later we have done a
weekly evaluation of the level of damages that presented the leaves of each plot and, by means of
statistical estimations, we have determined if any of the active ingredients used presents a significant
effectiveness of controlling the pest. It is equally important to detect if the entomofauna associated and
susceptible to control populations of D. mali, specifically Platygaster demades, is already present in our
territories and results a susceptible effective tool to be employed in the control of this pest as well. That
is why we have collected samples of affected shoots and tried to complete the biological cycle of the
insect on the laboratory to observe if in addition to obtain adults of D. mali appeared also adults of
Platygaster demades.
The results show that none of the active ingredients used has a clear efficiency against the insect and
also noted the presence of any parasitoid or predator capable to control the pest activity. It is necessary
to deepen in the study on the dynamic and the biology of D. mali to understand its functioning, know
how and which moments are better to act and to obtain some conclusive results
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Pomera -- Malalties i plagues
Plagues agrícoles -- Control
Cecidòmids
Apple tree -- Diseases and pests
Agricultural pests -- Control
Gall midges
Biología
Control de cecidòmia (Dasineura mali) (Dyptera, Cecidomyiidae) en plantacions comercials de pomeres: recerca bibliogràfica de la biologia de l'insecte i de les estratègies de control utilitzades en les àrees de producció i assaig de camp per validar l’eficàcia dels insecticides recomanats
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/118352016-01-12T01:00:54Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
García Marín, José Luis
García Ramos, María del Camino
Universitat de Girona. Facultat de Ciències
2016-01-11T15:49:33Z
2016-01-11T15:49:33Z
2015-09
http://hdl.handle.net/10256/11835
Birds are a very diverse animal group that is worldwide distributed in a heterogeneous manner.
Sometimes the influence of man has been one of the main causes that has generated losses of diversity in
birds, both directly (through hunting, overexploitation, etc.) and indirectly by fragmenting and altering
habitats where these species live. The objective of this Final Degree work is to analyze how genetics studies
related to birds have evolved over time, and particularly in relation to the threatened status of the studied
species and its economic interest. Also is has been able to analyze if both characteristics were related to
each other.
For the development of this project, three of the most relevant digital databases on this field have
been consulted. These are: WOS (Web of Science) of the ISI (Institute of Scientific Information) for search
the scientific papers, the Red List of the IUCN (International Union for Nature Preservation) and Birdlife
International to determine the species’ threatened status and its economic interest.
Through this study it is concluded that, over time, there is an exponential increase in the number
of published genetic papers on bird species. The undertaken statistical analysis showed how, over time,
there was not a significant increase in the proportion of studies based on IUCN endangered species. In the
same way, the proportion of studies focusing on species with economic interest remained constant over
time. In addition, it has been concluded that both variables ("threat level" and "economic interest") are
related. Over all revised papers, the highest proportion was observed in studies on non-endangered species
without economic interest, which suggests there are other reasons that promote the realization of genetic
studies on bird species. Nevertheless, a confluence between the studies on endangered species with
economic interest was also observed, but its proportion is still low
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Biologia (TFG)
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Ocells -- Protecció
Ocells -- Genètica
Birds -- Conservation
Birds -- Genetics
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Conservación o explotación: qué promueve la investigación genética en aves?
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oai:dugi-doc.udg.edu:10256/118362016-01-12T01:00:54Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Maraschiello, Ciriaco
Massaguer i Vall-llovera, Anna
Garrido Carmona, Beatriz
Universitat de Girona. Facultat de Ciències
2016-01-11T15:53:28Z
2016-01-11T15:53:28Z
2015-09
http://hdl.handle.net/10256/11836
Cholesterol is ubiquitous and necessary for the biosynthesis of steroidal hormones, bile salts and to maintain the stability of biological membranes in animal cells. Its regulation and homeostasis is ensured by the conjugated action of specific factors/enzymes/receptors such as SREBP, HMG-CoA or LXR. LDL and HDL are responsible to transport cholesterol and provide the cells to build and maintain the membranes, etc. Cholesterol, as lipids in general, is susceptible to oxidation and some positions in the chemical structure are more prone to oxidative attack; the most common cholesterol derivatives produced by oxidative attack are hydroxyl derivatives, keto derivatives, hydroperoxydes, epoxides and carboxyl derivatives. Those oxidized cholesterol derivatives are called Oxysterols or Cholesterol Oxides.
Cholesterol oxidation occurs by autoxidation processes in dietary food exposed to heating treatments in the presence of oxygen or have been stored for long periods and exposed to sunlight and oxygen. This autoxidation process constitutes an exogenous source of oxysterols i.e. they can be ingested from the diet. On the other hand, cholesterol oxidation can be mediated by enzymes belonging to the family of cytochrome P450 of oxygenases and autoxidation processes are also possible inside the body constituting both the endogenous source of oxysterols.
Oxysterols are characterized by a diverse profile of biological activities, including effects on sphingolipid metabolism, platelet aggregation, apoptosis, cytotoxicity, binding to specific transporters, relation with the Hedgehog pathway, binding to the oestrogen receptor and protein prenylation. The most notable oxysterol activity is related to the regulation of cholesterol homeostasis, which appears to be controlled in part by a complex series of interactions of oxysterols with various receptors, such as the oxysterol binding protein, the cellular nucleic acid binding protein, the sterol regulatory element binding protein, the LXR nuclear orphan receptors, and the low-density lipoprotein receptor.
Oxysterols are suspected to be involved in cholesterol homeostasis but also seem to play a role or at least are involved in the progression of neurodegenerative diseases and in carcinogenesis especially due to their interaction with cell death mechanisms (apoptosis) and because of their relationship with inflammatory processes.
Because of their relevant biological properties and their implication in important systemic processes and in the etiology or pathophysiology of certain diseases, this work provides clarifications on the role of Oxysterols in cholesterol homeostasis and provides insights on their direct or indirect role in the pathophysiology of Alzheimer’s disease and Cancer as well as if these could represent a direct or indirect target for therapeutic action.
The bibliographical investigation led to conclude that, currently, it is known that oxysterols are not the sole regulators of cholesterol homeostasis, although they are significant players in the regulation of this process. On the other hand, it has been demonstrated that they are involved in processes related to the onset and/or progression of neurodegenerative diseases such as Alzheimer, and with carcinogenesis. Because of this, Oxysterols could represent a big revolution in the field of biomedicine as potential direct or indirect targets for therapeutic action, but there is still the need of more in vivo experiments to translate their activities in humans and before deciding which strategy is applicable to develop a potential disease treatment
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Òxids de colesterol
Cholesterol oxides
Biología
Oxysterols: implication in biological processes and diseases
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/118372016-01-12T01:00:45Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Benito i Mundet, Antoni
Ramos Villullas, Israel
Marcet Puig, Laura
Universitat de Girona. Facultat de Ciències
2016-01-11T15:58:56Z
2016-01-11T15:58:56Z
2015-09
http://hdl.handle.net/10256/11837
The aim of the development of these investigation’s projects in Almirall is to find new molecules for a particular disease and it can get started in two different ways: the selection of a phenotype or of a protein targeting (GPCRs, enzymes, ion channels, etc.) whose modulation could become a benefit to a pathology of interest.
One of the proteins targeting that is currently raising more interest in the therapeutic investigation are the ion channels. The reason is that they play a critical role in the monitoring of many physiological processes. These processes form the molecular base of the nervous system, they also adjust the selective transport of the ions and macromolecules, they have a vital role in the synthesis of the mediators, they take part in the homeostasis and in the cellular communication. This project focus in the (VGSC). Nine sodium channels in humans have been identified and found nowadays that perform vital functions in the central nervous system and in the peripheral nervous system, as well. The dysfunction of these channels can create diseases which are called channelopathies such as the Brugada syndrome, childhood epilepsy or chronic pain.
Due to the required need in the discover of new drugs, the main objective of the study that was carried out for Almirall focused in the set-up of a new primary high-efficiency assay and minor costs than the electrophysiology using the platform FLIPR Tetra and monitoring the changes in the membrane potential, that could allow to characterize the power of new inhibitors of the sodium channel Nav1x. Once the set-up of the FLIPR Tetra was done, twenty-four inhibitors that were previously tested with the electrophysiology IonWorks quatro were characterized.
The results obtained with the FLIPR Tetra were in truth successful, the 87,5% being the most detected compounds in the same potency order than the electrophysiology. Moreover, the 75% of the detected compounds with a different potency order were more powerful than in the electrophysiology assay and the remaining 25% only seven times less powerful. This could assure its detection with the set-up assay. The controlling assay of the membrane potential using the FLIPR Tetra could be considered acceptable as a primary assay of characterization and narrowed down for specific inhibitors of the sodium channel Nav1.x, putting the electrophysiology as a activityconfirmation assay and characterization of inhibitors
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Canals de sodi
Canals iònics
Fluorescència
Electrofisiologia
Sodium channels
Ion channels
Fluorescense
Electrophysiology
Biología
Caracterización de inhibidores de la actividad de canales iónicos sensibles a voltaje: sensores de voltaje (fluorescentes) vs. electrofisiología
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/118392016-01-12T01:00:52Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Romaní i Cornet, Anna M.
Mora Gómez, Juanita
Torres i Roig, Meritxell
Universitat de Girona. Facultat de Ciències
2016-01-11T16:05:09Z
2016-01-11T16:05:09Z
2015-09
http://hdl.handle.net/10256/11839
The Riera Salada is an aquatic ecosystem characterized by having a high content of dissolved salts in some sections which come sediment basin where drift. The closest site to the birth of the stream has been sampled has a conductivity of 43.53 mS/cm and most distant (about 4 km downstream) of 0.60 mS/cm respectively. The objective of this study was to analyze the effect of the salinity gradient on the biomass of different microbial groups (bacteria, algae and fungi) that form the stream epilithic biofilm.
It also focused on determining its function by analyzing the enzymatic activities (phosphatase, β-gluco-sidase and leucineaminopeptidasa) that carried out bacteria, fungi and algae in order to compare whether there is a relationship between the function and structure of this biofilm. To reinforce the ob-tained data were analyzed the water nutrients and the dissolved organic matter (MOD). It has been found that the composition of the microbial groups in % has been favored by the algae, which has been the group most represented at each site, then by bacteria and finally the fungi. The results determined that there is an inhibition of microbial biomass micro-by salinity, being algae and bacteria, the most affected groups, and fungi, the group has presented more resistance throughout the gradient. However, in the section with higher salinity has been observed that there was a high microbial biomass due to the high content of nutrients because in this site there is a local eutrophication that probably counteracts the effect of salinity. Regarding the role of biofilms determined by the enzymatic activity has been seen that these were not seen very affected by the salinity gradient, being the β-glucosidase the most sensi-tive and less sensitive the phosphatase. Finally, leucineaminopeptidasa also presents certain relation-ship with the salinity but also is related nitrate content of the medium. The results suggest changes in the composition of microbial biofilm along the gradient studied, and therefore, further studies would be interesting to analyze microbial diversity and richness to see which species can be more or less re-sistant to salinity
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info:eu-repo/semantics/openAccess
Biofilms
Salinitat
Ecologia microbiana
Ecologia fluvial -- Catalunya -- Berguedà
Salinity
Microbial ecology
Stream ecology -- Catalonia -- Berguedà
Biología
Efectes d’un gradient de salinitat en l’estructura i el funcionament del biofilm epilític en una riera salada
info:eu-repo/semantics/bachelorThesis
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Verdaguer Murlà, Dolors
Ordeix i Rigo, Marc
Costa Sánchez, Sergi
Universitat de Girona. Facultat de Ciències
2016-02-18T08:22:08Z
2016-02-18T08:22:08Z
2015-09
http://hdl.handle.net/10256/12099
In this study we mainly conduct an assessment of the vegetation of the island of Gallifa or Sorral ( Location: River Ter, Masies de Voltregà, Osona), after the restoration project which took place in the island. This project was part of a bigger project to restore vegatation which currently aims at the riverbank of the river Ter, the project was led by Centre d’Estudis dels Rius meditarranis – Museu del Ter.
The island of Gallifa or Sorral is one of the few fluvial islands which are to be found in the upper-mid section of the river Ter. It is tangible evidence that the geomorphological activity of the Mediterranean rivers, along their meanders and floodable areas, make up habitats of great ecological potential.
Thus, in 2010 there was a felling of the artificial poplar (of Populus deltoides) plantation found on the Island. From then on, they started a project of restoration to recover the adequate vegetation of the area. To do so, they planted native trees of the area; they followed previous experimental design. The main objective of the project was to see in the long run whether it was better to plant vegetation or to let it grow on its own. This experimental design consisted of dividing the area into pieces of land which would then be restored. They just planted trees in half of those areas. In the pieces of land where they planted trees, they chose different species of trees, depending on the relative distance to the phreatic water level.
We have looked at the evolution of the survival of the planted trees, the differences between the native and exotic species and the pieces of land with and without planting schemes. We have compared the abundance of native trees, especially the white willow (Salix alba), taking into consideration the different types of habitats.
Generally, after 5 years, the riverbank forest has seen progressive improvement after the forest restoration were conducted. Additionally, even though the richness of species has not varied throughout the past 5 years, the plant structure in the riverbank forest has improved outstandingly, especially in the pieces of land where measures were taken and trees planted
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Ecologia fluvial -- Catalunya -- Ter (Curs d'aigua)
Ter (Catalunya : Curs d'aigua) -- Aspectes ambientals
Vegetació de ribera -- Catalunya -- Osona
Repoblació forestal -- Catalunya -- Osona
Stream ecology -- Catalonia -- Ter (River)
Ter (Catalonia : River) -- Environmental aspects
Riparian plants -- Catalonia -- Osona
Reforestation -- Catalonia -- Osona
Biología
Avaluació de la vegetació al projecte de restauració de l'Illa de Gallifa o del Sorral (riu Ter a les Masies de Voltregà, Osona)
info:eu-repo/semantics/bachelorThesis
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Bas Lay, Josep Maria
Puig, Xavier
Carbonell Agulló, Gerard
Universitat de Girona. Facultat de Ciències
2016-04-14T12:12:44Z
2016-04-14T12:12:44Z
2016-02
http://hdl.handle.net/10256/12331
The observation of wild mammals is so difficult because they are mostly nocturnal and
have wild customs. But you can always improve this detection and observation installing
a hide, to approach the wildlife to everyone. This kind of equipment is common in
Northern Europe, like England, where gives a good performance and is widely used by
all ages. Although in Spain are uncommon these hides, Naturaprop team has committed
to the infrastructure installed in Sant Esteve de Guialbes (Pla de l’Estany).
Seeing the high levels of carnivores in this hide, we raised the idea of a study the big
mammals around the most oriental part of Pla de l’Estany. By photo trapping, we have
collected data of the behaviour and the activity of the badger (Meles meles), beech
marten (Martes foina), genet (Genetta geneta), fox (Vulpes vulpes) and wild boar (Sus
scrofa). Also, to a lesser number, the roe deer (Capreolus capreolus) and european hare
(Lepus europaeus). These data allow us to know the daily and monthly activity patterns,
compared to other Palearctic regions, would be influenced by the biology of each specie,
potential availability of food, night hours or human activity. Also we tried to determine
the specific abundances of this area.
We have been seen that the most mammals studied presented bimodal activity, less
opportunistic mammals like foxes and wild boars. A monthly level, each specie follow a
different trend, and we think that basically, is due to mating and pregnancy season of the
different species. Moreover, the temperature in our latitude is not involved in their
activity. In the other hand, specific abundances have been determined, whenever it was
possible, with a total of least one badger, one beech marten, two genets, four foxes and
least five wild boars. Furthermore, we have seen a remarkable effect of hunting on game
species; however the other species don’t presents significant alterations along the
sampling process. About feral animals, the cats and dogs observed could have an
important position in the competition to get food with wild carnivores
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Mamífers -- Hàbits i conducta -- Catalunya -- Sant Esteve de Guialbes
Mamífers -- Poblacions -- Catalunya -- Sant Esteve de Guialbes
Mammals -- Behavior -- Catalonia -- Sant Esteve de Guialbes
Mammal populations -- Catalonia -- Sant Esteve de Guialbes
Biología
Patrons d’activitat dels grans mamífers a l’entorn de Mas Vidal (Sant Esteve de Guialbes; Pla de l’Estany)
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oai:dugi-doc.udg.edu:10256/128442016-10-12T00:00:51Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Figueras i Vall-llosera, Mercè
Silgado Giménez, Aroa
Universitat de Girona. Facultat de Ciències
2016-10-11T08:36:03Z
2016-10-11T08:36:03Z
2016-09
http://hdl.handle.net/10256/12844
Malaria is a worldwide health problem, where almost half of the world’s population is at risk of infection, and in 2015 it caused 500.000 deaths. This disease is has a global distribution, but mainly found in developing areas. One of the Sustainable Development Goals established by the WHOc for 2030 is to eradicate this disease. Thus, prevention and transmission control are the keys to getting it. This may be possible if there is a thorough and rapid diagnosis to establish the most appropriate treatment.
Despite the new diagnosis tools developed in recent years, optical microscopy remains the tool for excellence in the diagnosis of malaria. It has a high sensitivity and specificity if it is performed by a skilled and trained microscopist. Nevertheless, in endemic areas of the disease, most technicians of health centers are not familiarized with malaria parasites or do not have a high experience. Therefore, an automatic diagnostic system, which is based on microscopy but does not require the presence of an expert technician, is a good solution for these areas and it can help to control the disease and its transmission.
This automatic diagnosis is based on the development of a mobile application. For this, it needs an extensive database of images to train the application software and thus reduce false positives and negatives that may occur due to other forms similar to parasites. The images have been obtained from blood samples stained with Giemsa. In total 62 samples of the four species causing human malaria were examined to get the corresponding image.
1714 images were obtained between positive (1328) and negative (386). Most of the images obtained correspond to the specie Plasmodium falciparum because of its relevance in the disease. The first results of the application are encouraging because it is able to detect 80% of the parasites examined.
In conclusion, an automatic diagnosis allows rapid and careful diagnosis, and also lower cost than current and rapid detection techniques: a drop of 5 to 9 € versus 30 € for the rapid screening test
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Malària -- Diagnòstic
Imatges -- Processament -- Tècniques digitals
Malaria -- Diagnosis
Image processin -- Digital techniques
Biología
Desenvolupament d’una base de dades d’imatges microscòpiques per millorar el diagnòstic de malària mitjançant aplicacions digitals mòbils
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129762016-10-29T00:00:52Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Acuña Perandrés, Andrés
Universitat de Girona. Facultat de Ciències
2016-10-28T12:37:45Z
2016-10-28T12:37:45Z
2016-09
http://hdl.handle.net/10256/12976
Zucchini protected crops are increasing their relecance in the agricultural sector. However, the cultivation of this vegetable has a problem associated with pollination, attributed to the lack of pollinators, wich makes a much less productive crops. To date, farmers use fithormones that inducing the fruit, but consumers reject these methods, since this prodfuct remains in the fruit even when it is on the market. This study attempts to evaluate the ant species Pheidole pallidula (Nylander, 1849) as a possible vector of pollination in crops of zucchini, to avoid the use of more costly and/or less environmentally friendly techniques. This requires a count of pollen grains carrying the outgoing ants of male flowers and the incoming ants of the female flower. This count is performed over a period of time of 5 minutes. In addition, ot conducted an exclusion experiment on three groups of plants (G1: Plants with P.pallidula, G2: Plants with Lasius niger (linnaeus, 1758) and G3.
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Carbassons -- Conreu
Hormones vegetals
Pol·linització per insectes
Pollination by insects
Plant hormones
Zucchini
Biología
Pheidole pallidula como posible vector de polinización asistida en cultivos de calabacín (Cucurbita pepo L.)
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129792018-02-12T08:23:32Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Viñas de Puig, Jordi
López-Bermejo, Abel
Alarcón Masferrer, Xevi
Universitat de Girona. Facultat de Ciències
2016-11-02T09:02:17Z
2016-11-02T09:02:17Z
2016-06
http://hdl.handle.net/10256/12979
Delta-like 1 homolog (DLK1) gene encodes a transmembrane protein containing epidermal growth factor-like repeats that promotes the induction of mesenchymal cells but prevents chondrocyte, preadipocytes and osteoblast maturation and differentiation. Rs1802710 polymorphism situated in the fifth exon of DLK1, shows a significant parent-of-origin effect reflecting the known silencing of the maternally inherited copy by imprinting. In humans, a more frequent paternal transmission of the paternal allele in the rs1802710 polymorphism of DLK1 on obese children has been noticed. We aimed to study the single nucleotide polymorphism (SNP) rs1802710 of the DLK1 gene and its effects on early life development. Due to the known imprinting effect surrounding the DLK1 locus, we wanted to analyze whether these changes were different depending on the parental origin of the transmitted rs1802710 variant T allele. Therefore, a longitudinal study has been done from the beginning of the pregnancy to the 12th month-old baby after birth. In order to be able to detect parent-of-origin effects, the whole trio family has been genotyped. Those families in which we were able to track the newborn rs1802710 T allele origin, were the ones selected for the complete analysis. Maternal anthropometric and metabolic variables, DLK1, MEG3 RTL1 and DIO3 gene expression, likewise newborn prenatal, perinatal and postnatal anthropometric variables were assessed to analyses differences when the the variant T allele was paternally or maternally transmitted to the newborn. DLK1 rs1802710 paternally expressed T allele was independently associated with low levels of maternal fatty acid synthase (β=0.415, p=0.024; model R2=0.142), generalized fetal growth rate increment (β = 0,268, p = 0,003; model R2 = 0.559), increased birth cranial perimeter-SDS (β=0.202, p=0.048; model R2=0.045) and with decreased ponderal index at 1st month of age (β=0.434, p=0.003; model R2=0.148). No significant differences were observed when the T allele was maternally received Those results highlight the paternal-origin effect that described in the DLK1 locus region
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
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Regulació genètica
Polimorfisme genètic
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Metabolisme -- Trastorns
Metabolism -- Disorders
Gene expression
Genetic polymorphisms
Genetic regulation
Biología
Paternal transmission of the variant allele T for the RS1802710 single nucleotide polymorphism in DLK1 gene: relation with prenatal and postnatal growth
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oai:dugi-doc.udg.edu:10256/129802016-11-03T01:01:05Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Poater Teixidor, Albert
Arnedo Marimon, Laia
Universitat de Girona. Facultat de Ciències
2016-11-02T09:22:00Z
2016-11-02T09:22:00Z
2016-09
http://hdl.handle.net/10256/12980
Density functional theory calculations (DFT) have been used to describe the first
turnover for olefin metathesis reaction of a new in silico family of homogenous Ru-based
catalysts bearing a phosphoylide ligand with ethylene as a substrate. Equal to conventional Rubased catalysts for olefin metathesis that bear a N-heterocyclic carbene (NHC) ligand, the activation of these congeners occurs through a dissociative mechanism, with a more exothermic first phosphine dissociative step. However, predicted upper energy barriers were calculated to be on average ~5 kcal/mol less beneficial with respect to the Ru-NHC based catalysed metathesis. Overall, this present computational study emphasises on advantages of bidentate ligands to replace the NHC ligand of Ru-based metathesis and gives a potential recipe for the design of efficient Ru-based olefin metathesis catalysts. The new generation of catalysts is cationic, however the addition of a chloride instead of the phosphine seems to be
favourable and with better performance in terms of both kinetics and thermodynamics
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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Metathesis (Chemistry)
Ruthenium Catalysts
Alquens
Metàtesi (Química)
Catalitzadors de ruteni
Biología
Nova generació de catalitzadors de metàtesi d'olefines: la substitució de NHC per ilur de fosfoni
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129822017-10-10T13:59:40Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Campos i Llach, Miquel
Bagaria Osuna, Marc
Universitat de Girona. Facultat de Ciències
2016-11-02T09:48:47Z
2016-11-02T09:48:47Z
2016-09
http://hdl.handle.net/10256/12982
Freshwater mussels (Mollusca, Bivalvia, Unionoidea) are one of the most threatened animal group in Catalonia. Therefore, artificial culture has been established as the main measure for their conservation. LIFE project Potamo Fauna is working on the population's conservation of Unio mancus Lamark, 1819 and Unio ravoisieri in Girona's rivers based on an in vivo culture method in which it is used glochidia to infest host fish under controlled laboratory conditions.
The aim of this paper is to put into practice the in vitro method to culture Unio mancus artificially, which allows glochidia to undergo their metamorphosis without the use of a host fish. This method consists on culturing glochidia in an incubator under controlled temperature and CO2conditions, in an artificial culture medium containing M199, carp or rabbit serum, and a mixture of antibiotics and an antifungal. The objectives of this work are to determinate which of the two types of serum (carp or rabbit) is better for the culture medium, and also to determinate if a previous treatment of glochidia with malachite green has an effect on the survival or fungal contamination of the culture. Previously, the dose of malachite green used in the treatment will be defined, as well as the appropriate pH, CO2 and temperature conditions for the culture.
Glochidia were tracked, and when they metamorphosed into juveniles, their metamorphosis success was calculated. With the obtained results once the culture is done, it has been determined that the best type of serum for the in vitro culture is the one from carp, because it allows a greater metamorphosis success for the glochidia during the culture, as well as presenting less fungal contamination in comparison with rabbit serum. It has also been determined that a treatment with malachite green has not any effect on the metamorphosis success of glochidia or fungal contamination of the culture medium. Finally, it is important to highlight the fact that it is the first time that juveniles of the species Unio mancus are obtained through an in vitro culture method
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Nàiades (Mol·luscos) -- Reproducció
Freshwater mussels -- Reproduction
Biología
Cultiu in vitro de nàiade allargada (Unio mancus Lamarck 1819) en condicions de laboratori
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129852016-11-03T01:00:52Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Casadevall, Margarida
Barceló Armada, Andrea
Universitat de Girona. Facultat de Ciències
2016-11-02T11:03:15Z
2016-11-02T11:03:15Z
2016-06
http://hdl.handle.net/10256/12985
White seabream, Diplodus sargus, is a species of Sparid that exists in the Mediterranean sea and habits the rocky infralittoral and the Posidonia oceanica seagrass. In the past 10 years, there have been reported an increase of strange specimens: they became hard after cooking, and make them non eatable. That has never been seen on any other species of Diplodus. Despite the caulerpin has been described as the cause (which is a toxic invasive algae Caulerpa cylindracea (before called Caulerpa racemosa var. cylindracea)), this hypothesis may not be applied to the catalan coast, because there are no important reports of the algae’s emergence. In the current study, we have examined the stomach and intestinal content of 26 white seabreams of different sizes in order to disprove this hypothesis and so we can consider new hypothesis to describe what causes that anomaly. The results of the feeding study match the current literature: white seabream is a not specialist fish, and has an omnivorous and strongly varied diet which depends on different factors like the seasonality or the geographical area. It has been determined what animal preys and what ingested plants show more frequency and abundance in the diet. The most habitual species can’t be the observed anomaly’s cause, because nor animal preys or none of the habitual ingested plants have toxicity. Attending to the distribution analysis of the captured specimens which show the problem, we formulate diverse hypothesis of other possible causes, related with the species’ feeding strategy, but not with the particular foods
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http://creativecommons.org/licenses/by-nc-nd/3.0/es/
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Peixos -- Cria i desenvolupament
Fishes -- Breeding
Biología
Estudi de l’estratègia alimentària del sarg, Diplodus sargus (F. Sparidae, Pisces)
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129902016-11-03T01:01:06Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Roura i Pascual, Núria
Casas Cros, Pere
Universitat de Girona. Facultat de Ciències
2016-11-02T12:06:04Z
2016-11-02T12:06:04Z
2016-09
http://hdl.handle.net/10256/12990
Nowadays, the conservation of natural areas is a major issue in our society, but most of efforts and resources are focused on the most important ecosystems such as great forests and coral reefs because they are places of high biodiversity and where habitat destruction is very high. But there are also other ecosystems like seminatural grasslands presenting great biodiversity, but currently we did not known a lot about their ecology and how animal communities respond to the degradation of the vegetation. The study was carried out in the Parc Natural Zona Volcànica de la Garrotxa (PNZVG) which contains 2.5% of natural grasslands, with the aim of testing the influence of the floristic quality of the meadows on the diversity of arthropods to know how manage this systems. The identification of the arthropods was carried out at morphospecies level due to the high complexity of reaching the level of species. The results show that the main epigea arthropods orders in the PNZVG are Aranea, Coleoptera, Hemiptera and Hymenoptera. Comparing the arthropods at the level of order according to the temporality of the trail, the floristic quality of the meadow and the different grasslands studied, we observed significant differences between the diversity of orders and the floristic state of the meadows, being the meadows in worst conditions above the meadows in good condition, which is opposed to the initial hypotheses in the grasslands with better state we expected more diversity. At the level of morphospecies we found no significant differences in most cases, we only could demonstrate that the different fields studied differed significantly among themselves regarding abundance, diversity and richness. However, selecting only the four major orders morphospecies we found that in addition to the differences between the meadows, there are significant differences between the temporality and abundance of arthropods between 9 and 23 July to 6 August due to changes in rainfall and temperature caused a decrease in the activity of arthropods. We should continue with the monitoring of the arthropod community to better understanding of its dynamics and how varies this comunity depending on the floristic quality of seminatural grasslands.
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Arthropod populations
Grassland ecology
Artròpodes -- Poblacions
Ecologia de les praderies
Biología
Com influeix la qualitat florística dels prats seminaturals en la diversitat d’artròpodes
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Roura i Pascual, Núria
García Galindo, Blanca
Universitat de Girona. Facultat de Ciències
2016-11-02T13:04:24Z
2016-11-02T13:04:24Z
2016-09
http://hdl.handle.net/10256/12993
The scientific collections represent the nature diversity and are part of the heritage of biology, especially those that contain bugs, since they are the richest biological species group. And for a time, the Serra family, from La Garrotxa, gave a collection composed mostly by moths, butterflies and other insects in the area. As this collection was not listed, this study aims to identify all the individuals that compose it according to their order, family, genus and species.
By Lepidoptera (butterflies and moths) there are established two groups, which generally are ranked according to whether they are Rhopalocera (daily) or Heterocera (nightly) because the morphology of individuals and the families has varied greatly due to their operating hours. Then, the entomological pins were labeled with family, genus and species, handwritten. The other insects (Coleoptera, Hymenoptera, Diptera...) were labeled pointing the order due to the difficulty in identification by gender and species.
The results show that the collection has high species richness with many different families of Lepidoptera, thus confirming that in the Girona region there is a biological heritage interest
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Arnes
Papallones
Moths
Buterflies
Biología
Identificació i catalogació de la col·lecció de papallones, i altres insectes, procedent de la donació particular de la família Serra (Olot; Garrotxa) a la UdG
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129942017-10-10T13:46:21Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Garcia Garin, Odei
Universitat de Girona. Facultat de Ciències
2016-11-02T13:33:15Z
2016-11-02T13:33:15Z
2016-06
http://hdl.handle.net/10256/12994
Semi-natural grasslands are habitats that have not been sown by the hand of man, they show an important species richness, they have a great ecological and productive importance and, in addition, they show notable environmental functions. Only 2% of the Parc Natural de la Zona Volcànica de la Garrotxa (PNZVG) is constituted by semi-natural grasslands, moreover, they can quickly evolve depending on environmental changes and/or management, therefore, it is highly recommended to keep the information about its conservation state updated. One of the most used groups of invertebrates as a biologic indicator and that comply all the features to be a good bioindicator is the group of ants, because they are very important at an ecologic level, they carry out many functions inside the ecosystem, ants’ diversity in the world is high, they are found in abundance in most terrestrial ecosystems, they are relatively sedentary, they are sensitive to environmental change and are easily sampled, classified and identified. Thus, in order to study the conservation state of grasslands in more detail, our aim here is to verify if the ant community is a good biological indicator to determine the semi-natural grasslands’ floristic quality of the PNZVG and, at the same time, to study the ant community at functional group levels. The results show that up to 28 species have been identified in the semi-natural grasslands of the PNZVG. The results obtained on ants diversity and the other community variables in regards to the floristic quality of the semi-natural grasslands are not significant, although we could intuit a tendency to have more ant richness and diversity in higher semi-natural grasslands floristic quality. In functional group terms, in the semi-natural grasslands of the PNZVG there are 46% of disturbance bio indicator species, 43% of maturity bio indicator species and 11% of cryptic species, meaning that the disturbance and maturity indicators are even because they are managed by grazing or haymaking and, therefore, they are under the influence of a certain disturbance. The use of the ant community as a bio indicator of the semi-natural grasslands’ floristic quality of the PNZVG should confirm or refute over time when we make use of the methodology in a monitoring program for 1-3 years
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
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Insect-plant relationships
Relacions insecte-planta
Formigues
Ants
Biología
Les formigues com a indicador biològic de la qualitat dels prats del Parc Natural de la Zona Volcànica de la Garrotxa
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/129992016-11-04T01:00:59Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Molina López, Rafael Ángel
Garcia Recasens, Alexandra
Universitat de Girona. Facultat de Ciències
2016-11-03T09:34:37Z
2016-11-03T09:34:37Z
2016-06
http://hdl.handle.net/10256/12999
The orphans of commonswift (Apus apus) represent a high number of admissions in
Recovery Centers of Fauna in Europe. These centers have become an important
sentinels of the wild animals species, contributing actively to its conservation. In these
centers a lot of protected species are rehabilitated and hand-reared being the
commonswift the most representative. Exist studies about the results of the
rehabilitation and the hand-rearing of this specie in other countries but in the Iberian
Peninsula there aren’t data available, is for that, that this study was realized in the
Recovery Centre of Fauna Torreferrussa (Barcelona).
The chicks and fledgings (orphans) of commonswift represent the largest volume of
individuals that have to be hand-reared and rehabilitated, for that, the admission data
of 1903 orphans that were looked after in the Center during the year 2015, were
revised.
The study was designed to describe the results of the rehabilitation and hand-rearing of
commonswifts enough to meet and evaluate if there were differences between the
ages, the clinic categories and the resolutions, in addition to know of the existence of a
unique criterion of assignation of categories for all the rehabilitators, since the first
assessment of the situation of each individual would be reflected in its resolution.
The results of the rehabilitation and hand-rearing were positive in the orphans group,
showing more than a 50% of the orphans released. But, on the contrary were shown
significant differences between the clinics categories, the type of resolution and also
between the valuations of the rehabilitators. Therefore, this research exhibits the need
to established a common criterion for all the rehabilitators to take unanimous decisions,
that at the same time it will be reflected in a best quality and effort on the task of handrearing and rehabilitation of this threatened species, which will be shown as an
increase in the number of liberations at the end of the commonswift (Apus apus)
breeding season
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Ocells -- Protecció
Ocells -- Cria i desenvolupament
Falciot negre
Common swift
Birds -- Conservation
Birds -- Breeding
Biología
Aspectes clínics de la rehabilitació de falciot negre (Apus apus) al Centre de Fauna de Torreferrussa (Barcelona) durant l’any 2015
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130002016-11-04T01:01:00Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pons Ferran, Pere
Godoy Recasens, Robert
Universitat de Girona. Facultat de Ciències
2016-11-03T10:12:28Z
2016-11-03T10:12:28Z
2016-06
http://hdl.handle.net/10256/13000
Tibicina quadrisignata is a species of the family Cicadidae which is distributed along the south of Europe (populations have been described in France, Catalunya and Portugal) that inhabits in arboreal and high thickets zones. Even though it is a common group, the Cicadidae haven’t been studied deeply and the effects that disturbances could cause on their habitat are unknown. Wildfires cause a great destruction of the habitat in which these species are found, and it can affect in different ways their survival. Specifically, this project aims to detect the possible variations of the microhabitat where Tibicina quadrisignata is found after a wildfire, and how the visual depredation is affected after this disturbance destroys partially or totally the natural shelter that represents the foliage of the vegetation. The team of the research project ANIFOG of the University of Girona recollected data of the microhabitat where the individuals where found in order to analyze the differences between burned and unburned zones by the wildfire of the Alt Empordà during the summer of 2012. In order to analyze the visual detection of the predators, a test was done to volunteers who had to discover the species in photographs of the specimens in their microhabitat and in both zones. This test increases the sample size of the test used by G. Banchs 2015 within her TFG. Significant differences have been found in the support diameter, which was higher in burned forests. Furthermore, differences between the height in which specimens were found were also significant, and appeared to be higher in the unburned zone. On the other hand, clear differences weren’t found between the detection of T. Quadrisignata. Individuals of the unburned zone appeared to have a the same percentage of detection, which is contradictory to what initially would be expected. This fact can be explained due to the dark color that T. quadrisignata has, which would grant them a mimetisation vantage in burned zones. The blackening of the bark of the vegetation brings as a consequence the confusion of the observer, who acts as a predator, of the specimen with the support where it is on. The increment of the diameter would work as a vantage, because it would increase the surface where the individual can mimetize. This results show that the effect of wildfire gives Tibicina quadrisignata a vantage when it has to visually hide from the predators, which compensates the loss the shelter that provides the foliage
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
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Hemípters
Hemípters -- Efecte dels incendis -- Catalunya -- Alt Empordà
Depredació (Biologia)
Hemiptera -- Effect of fires on -- Catalonia -- Alt Empordà
Predation (Biology)
Biología
Estudi de les diferències de microhàbitat i de la vulnerabilitat a la depredació visual de tibicina quadrisignata en boscos cremats i no cremats
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130012021-06-07T09:34:21Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Cibils, Luciana
Romaní i Cornet, Anna M.
González Gámez, Juan
Universitat de Girona. Facultat de Ciències
2016-11-03T10:30:23Z
2016-11-03T10:30:23Z
2016-09
http://hdl.handle.net/10256/13001
The Riera Salada is a unique river ecosystem, as it presents a very high salinity of natural origin, being the highest peak (at the start of the river) of 43.53 conductivity mS/cm approximately three times more than the conductivity of the sea. The high salinity is caused by evaporites of Keuper, sediments that are found in different sites of the river. One of the curious facts is the strong gradient of salinity which results as a consequence of the successive entrance of water of the tributaries. At the end point of the sampling it falls down to 0.60 mS/cm, the conductivity of a freshwater river. The main objective is to see and identify which species inhabit these conditions (halophilic conditions) and how they may vary along the salinity gradient, as to identify if there are significant differences according to the different substrates of the river bed (sediments, pebbles and leaves). In this study they are especially observed the primary producers, algae and diatoms. As they were the most representative group as a percentage of abundance, a specific protocol for their observation was realised. It consisted to burn all the remains of organic matter and leave only the frustules of the diatoms for their observation under the microscope. From the various data have been calculated diversity index. It has been found that the salinity is not the determining factor, but it is the presence or absence of nutrients, it seems that the absence of nutrients affects more to the diversity and abundance than salinity does. It is also noticed that the substrate with more chlorophyll and diversity are the leaves, and then the boulders.
It appears that the most representative organisms are diatoms followed by Bangia
atropurpurea and Enteromorpha, halophiles species present in most salted sites. Within the group of diatoms the most abundant genus is Acnanthes. The results suggest significant changes in the composition of the biofilm between the sampling points and the type of sediment.
Finally we noted that the substrate most affected by salinity is sediments, although the
diversity index are equal, the community changes a lot of species and genera
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Biofilms
Salinitat
Ecologia microbiana
Ecologia fluvial -- Catalunya -- Berguedà
Salinity
Microbial ecology
Stream ecology -- Catalonia -- Ter (River)
Biología
Efecte del gradient de salinitat en la comunitat del biofilm de la Riera Salada
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130042016-11-04T01:01:01Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Benito i Mundet, Antoni
Castells, Antoni
Gratacós Mulleras, Anna
Universitat de Girona. Facultat de Ciències
2016-11-03T11:15:48Z
2016-11-03T11:15:48Z
2016-09
http://hdl.handle.net/10256/13004
Colorectal cancer (CRC) is the most frequent neoplasia in both sexes in Spain. It may be sporadic (65%) as well as familial (35%). In the majority of cases the cause of familial CCR remains unknown. However, a 5% is due to high penetrance germline mutations which are characterised as hereditary Mendelian syndromes, such as Lynch syndrome, familial adenomatous polyposis and the poliposis associated with MUTHY and POLE/POLD1. Nowadays using next generation sequencing techniques, new genes associated with germline predisposition to CRC such as NTHL1 or FAN1, have been identified.
The main objective of this study is the identification of new genes related to germline predisposition to CRC. Families with a strong aggregation for the disease and the selection of variants shared by different individuals in each family were studied. Germline DNA samples used for the whole exome sequencing by Illumina method were obtained from peripheral blood of patients with CCR belonging to 16 unrelated families. A bioinformatic analysis was performed for reducing the number of encountered variants and selecting those with a high probability to cause a change in the gene function. The candidate genes in each family have been selected considering several functional parameters. Finally, a validation of the variants was carried out (Integrative Genomics Viewer and Sanger sequencing).
Twenty-seven variants were selected and 24 of them validated by Sanger sequencing. Segregation studies from additional family members who presented advanced adenomas or other neoplasms as well as somatic studies with tumour DNA to determine the loss of heterozygosity (LOH), were also performed. The most remarkable results were found in 9 genes: LOXL4, GEMIN5, UTP11L, MUC6, HIST1H4, GSK3A, SPLIT2, CIDEB and MCM3. According their function or previous relation with cancer LOXL4, HIST1H4, GSK3A and MCM3 have been considered the most relevant.
We identified new possible germinal predisposition variants associated to CCR in genes that play a role in cell cycle, apoptosis and DNA repair., Furthermore, LOXL4, which is associated with head and neck cancer, is also related to CCR. Similarly, MCM and SLIT gene families could participate in genetic CCR predisposition
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Còlon -- Càncer -- Aspectes genètics
Marcadors bioquímics
Biochemical markers
Colon (Anatomy) -- Cancer -- Genetic aspects
Biología
Identificació de nous gens implicats en predisposició germinal al càncer colorectal
info:eu-repo/semantics/bachelorThesis
east=2.1508767799065254; north=41.38859016327313; name=Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)
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oai:dugi-doc.udg.edu:10256/130082016-11-04T01:01:16Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Roldán Borassi, María Inés
Ramos Blanes, Rafel
Elosua Bayés, Marc
Universitat de Girona. Facultat de Ciències
2016-11-03T11:41:09Z
2016-11-03T11:41:09Z
2016-06
http://hdl.handle.net/10256/13008
Cardiovascular diseases are the main cause of mortality and morbidity in high-income countries being arteriosclerosis the main pathological mechanism involved. The cardio-ankle vascular index (CAVI) is a novel stiffness measurement used to assess the extent of arteriosclerosis that enables us to detect it at early stages allowing for timely prevention treatment. In this study we want to describe the CAVI values in a Mediterranean population, as well as its associations with different clinical and behavioral risk factors. Additionally we want to analyze its relation with carotid arteriosclerosis burden assessed by other methods. A cross-sectional study was conducted with a representative sample of the general population of Girona between 40-90 years old (n=2502). The variables analyzed were: i) classical risk factors: hypertension, diabetes, obesity and hypercholesterolemia; ii) life styles: diet quality, smoking habits and physical activity; iii) carotid arteriosclerotic markers: common carotid artery intima-media thickness (CCA-IMT) and distensibility coefficient (DC); and iv) CAVI.
The group with abnormally high CAVI consisted of 43.32% of our sample and had a higher prevalence of cardiovascular risk factors, except for obesity. They also had a higher proportion of non-smokers, as well as individuals who didn’t achieve the recommended cardio-protector physical activity, while no differences between groups were observed regarding the diet quality. A significant moderate correlation between CAVI and CCA-IMT and the DC was observed. We found a high prevalence of abnormally high arterial stiffness in a low coronary risk population. Since arterial stiffness is not included in the coronary risk scores, CAVI could be used to improve the classification of intermediate risk individuals sorting out those with higher risk that nowadays go undetected targeting them for timely prevention. CAVI has shown to be independently associated with aging, male sex and classical risk factors except for obesity where an inverse association was found. Physical activity in men was the only lifestyles independently associated with CAVI and did so with lower levels of CAVI. CAVI has also moderately correlated with carotid atherosclerotic burden assessed with CCA-IMT and DC
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Biologia (TFG)
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Sistema cardiovascular -- Malalties -- Factors de risc
Cardiovascular system--Diseases --Risk factors
Biología
Arterial stiffness in a Mediterranean population: variation of cardio-ankle vascular index related to the presence of traditional cardiovascular risk factors and CV risk score
info:eu-repo/semantics/bachelorThesis
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Barrabés Vera, Sílvia
Fernández López, Pol
Universitat de Girona. Facultat de Ciències
2016-11-03T12:12:13Z
2016-11-03T12:12:13Z
2016-06
http://hdl.handle.net/10256/13011
Cancer is nowadays a global health problem constantly increasing, being the second cause of death in developed countries. Pancreatic adenocarcinoma, often referred as pancreatic ductal adenocarcinoma (PDAC), is the fourth leading cause of cancer death in Europe and EEUU with a mortality tax greater than 90%, even though it only represents a 3-6 % of cancer cases within population.
The glucidic alteration associated to tumoral processes is a well described feature. In this context, the overexpression of the glucidic antigens Sialyl Lewis X (SLeX) and Sialyl Lewis A (SLeA) in PDAC, significally enhances the aggressiveness of the pathology (more migration, invasion and metastasis capability).
The synthesis of these antigens is complex and involves the sialyltransferases (STs) and other enzymes. STs are enzymes that place sialic acids (Sias) in terminal position of glucidic chains. The sias are diverse in structure, but they all are negatively charged, promoting de-adhesion of tumour cells by charge repulsion (and thus, contributing to metastasis formation).
In this work, the attention is centred on SLeX and SLeA antigens, and on ST implicated in their formation: ST3Gal III and ST3Gal IV. In this line, a lack of these enzymes should directly mean a reduction of the antigens and the metastatic behaviour of the cells.
The starting point is having different cell lines of PDAC: parental (non-silenced), and silenced in ST3GAL3, ST3GAL4 or in both genes. Thus, the objective is to define an experimental design by searching bibliography, allowing to, firstly, characterise the silencing, and secondly, characterise the metastatic phenotype. Attempting to achieve this objective, different analyses have been proposed.
The first part includes: quantification of protein expression levels (by western blot, in order to quantify the STs), quantification of the antigens (by flow cytometry against the SLeX and SLeA structures) and a viability assay (MTT), with the intention to check out if lack of STs or SLeX and/or SLeA antigens aren’t a significant compromise for cell viability. These analyses make up the silencing characterisation
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http://creativecommons.org/licenses/by-nc-nd/3.0/es/
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Adenocarcinoma
Pàncrees -- Càncer -- Investigació
Pancreas -- Cancer -- Research
Biología
Disseny experimental per a la caracterització de models cel·lulars amb els gens ST3GAL3 i ST3GAL4 silenciats
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Gómez López, Crisanto
Marrero Pérez, Jacobo
Rovira Victoria, Carla
Universitat de Girona. Facultat de Ciències
2016-11-03T12:38:37Z
2016-11-03T12:38:37Z
2016-09
http://hdl.handle.net/10256/13013
The angel shark (Squatina squatina) is one of the most endangered species in the world, actually being at the top one hundred (Dulvy et al., 2014), catalogued as critically endangered by the IUCN in 2006. The decline on its population began just over 10 years ago, mainly caused by a large fishing pressure over its original range. Other factors such as habitat loss caused by humans or certain change in some features of their ecology make them even more vulnerable. At present they can only be found in areas of the southern Mediterranean and the Canary Islands.
Elasmobranches nursing areas are considered essential habitats for survival ("Essential Fish Habitat" EFH) or critical habitats (Beck et al., 2001). It has recently been discovered that the beach Las Teresitas (Tenerife, Canary Islands) are nursing areas for S. squatina (Escánez et al., 2013)
In this project we have studied different factors, both biotic and abiotic, that might be influencing the distribution of juveniles. The aim of this study is to determine if these factors are significantly important and influential in the spatial distribution along the nursery area.
Samples have been taken following the seasonal visual census methodology or "fixed point" method (Bortone et al., 1989; Falcon et al., 1996) which consisted on the collection of data of abundance potential prey, the substrate hardness and the water temperature at different distances from where the juvenile shark was.
On the one hand, the results and their analysis (Kruskal-Wallis test) for the factors of water temperature and substrate hardness revealed no significant difference, concluding that none of them is decisive in the distribution of juveniles. On the other hand, the analysis of the abundance of prey does not give such a concise answer. Although the Kruskal-Wallis test suggests a non significant difference, several graphical representations of the data show a downward trend in the number of dams with increasing distance to breeding. In addition, this fact seems to be accentuated with high value of abundance of prey (5 or more ind.). These results lead to further discussions as to where new issues can be addressed in the future. Different strategies focused on the methodology used, with modifications and alternatives to continue the research
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Squatina squatina
Taurons -- Hàbits i conducta
Taurons -- Distribució geogràfica
Sharks -- Geographical distribution
Sharks -- Behavior
Biología
Estudio sobre la influencia de los factores de temperatura del agua, dureza del sustrato y abundancia de presas en la distribución espacial de crías de Squatina squatina en su área de cría (playa de Las Teresitas, Tenerife)
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oai:dugi-doc.udg.edu:10256/130142016-11-04T01:01:22Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Casadevall, Margarida
Rogister, Bernard
Sánchez Gil, Judit
Universitat de Girona. Facultat de Ciències
2016-11-03T12:58:09Z
2016-11-03T12:58:09Z
2016-06
http://hdl.handle.net/10256/13014
SV2s family proteins are neuronal vesicles membrane glycoproteins that are highly conserved in evolution (Janz & Südhof, 1999). It is known that SV2A isoform is the target to Levetiracetam (LEV), an effective anti-epileptic drug (B. Lynch et al., 2004). There are certain hypotheses about his role in epilepsy. However, his physiological function and its interaction with LEV remains unknown. Specifically, it is known that a specific type of Temporal Lobe Epilepsy (TLE) presents a reduction of SV2A expression associated with an upregulation of SV2C isoform (Crèvecoeur et al., 2014). The aims of this study consist in validating the proper operation of SV2A lox/lox sequence in order to invalidate SV2A expression and, secondly, analysing the expression of SV2B and SV2C in the absence of SV2A. For this purpose, a mouse line allowing the conditional removal of SV2A in the hippocampal region (CA3 and dentate gyrus (DG)) at postnatal stages 15 (P15) (Grik4:SV2A-cKO) was engineered. Unexpectedly, Grik4: SV2A-cKO transgenic mice did not present an epileptic phenotype. To determine the reason of these phenomenon, the concentration of the three isoforms was analysed and quantified. Results have shown a significant reduction of the quantity of SV2A transcript and
protein in Grik4:SV2A-cKO animals hippocampus compared to the wild-type mice (WT).
Together, these results confirmed the efficiency of the invalidation of SV2A in our mouse
model. Parallel to these values, no significant change in SV2B or SV2C protein and transcript expression was observed implying an absence of compensation phenomenon. Nevertheless, many other hypotheses should be tested to explain this unexpected phenotype in Grik4: SV2AcKO mice. Finally, in order to resolve the role of SV2A in synaptic plasticity, a new nucleofection protocol for embryonic cortical neurons at the embryonic stage 16 (E16) in vitro was developed
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
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info:eu-repo/semantics/openAccess
Transgenic mice
Epilepsy
Hippocampus (Brain)
Hipocamp
Epilèpsia
Ratolins transgènics
Biología
Role of the SV2A protein in epilepsy and Alzheimer's mouse models
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130232018-11-16T08:59:17Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Casadevall, Margarida
Rogister, Bernard
Solés Tarrés, Irene
Universitat de Girona. Facultat de Ciències
2016-11-03T15:04:34Z
2016-11-03T15:04:34Z
2016-09
http://hdl.handle.net/10256/13023
The sardine (Sardina pilchardus) is a pelagic cold-water fatty fish species usually caught in the Mediterranean and it is a very healthy species because of its high content of omega-3 fatty acids. It is well known that the sardine is not usually infected by Anisakis nematodes, which are the origin of some human allergies and whose presence is very common in commercial species. During the last few years, an exhaustion of the sardine has been observed: its capture and quality have decreased. For this reason, and because of its importance in gastronomy, economy and health, we have focused the project on this species. In order to do so, the study has been divided in three sections: the physiological condition of the fish, the parasitological study and finally the analysis of the commercial durability.
In relation to the physiological condition of the sardine, it has been found out that it is now under what is considered a good condition; the cause should be attributed to external factors (climate change and overfishing), and internal factors related to the biology of the species, since when the study was carried out, the sardines were in the active phase of their reproductive cycle.
Related to the parasitological analysis, the absence of the Anisakis parasites has been demonstrated. In fact, only the nematode Hysterothylacium aduncum and some Digenea trematodes have been identified. However, it has been detected a prevalence and intensity of H. aduncum in the third larvae stage (L3) and in the advanced third stage larvae (L3-4), which has never been reported in the sardine. This fact may produce negative effects on human health, bearing in mind that some allergy cases in humans have been related to this parasite. On the other hand, the infection of H. aduncum can cause the sardine’s exhaustion and an increase in its susceptibility to infections. Nevertheless, in this study we didn’t find significant differences between the physiological condition of infected sardines and not infected sardines.
Finally, for the study of the conservation and durability of the fish, we have applied the qualitative sensory method, called QIM. Results showed that the most indicative attributes, in order to value the quality of the sardine, are the odour of the gills, the eyes’ appearance and the state of the abdomen. Moreover, it has been determined that
the shelf-life of the sardine is about 3-4 days, and the importance of the way the fish is kept since the capture has also been demonstrated. Finally, in order to prolong the shelf-life of the sardine it has been proposed to improve quality by washing the fish just after it has been caught, which has been demonstrated effective in other species
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Sardines
Sardines -- Ecologia
Sardines -- Paràsits
Productes pesquers -- Conservació
Fishery products -- Preservation
Indústria sardinera
Sardine industry
Biología
La Sardina (Sardina pilchardus): estudi de la condició fisiològica, parasitologia i durabilitat comercial
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130242016-11-05T01:00:56Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Feo, Carles
Vila Belmonte, Mar
Universitat de Girona. Facultat de Ciències
2016-11-04T09:26:55Z
2016-11-04T09:26:55Z
2016-09
http://hdl.handle.net/10256/13024
The European pond turtle (Emys orbicularis) is a species of freshwater turtle that is distributed throughout much of Europe. Despite having a wide distribuXon, many of their populaXons suffer a major setback, causing their fragmentaXon and disjointaXon. The InternaXonal Union for ConservaXon of Nature (IUCN) has considered this
species, since 1996, near threatened (NT) on a global level. In Spain, this species has a vulnerable state (VU), although populaXons Northwest and East are considered endangered (EN). E.orbicularis is listed as strictly protected under Annex II of the Berne ConvenXon and Annex I and Annex II of the Habitats DirecXve EEC. The situaXon of threatened
species of E.orbicularis requires the implementaXon of projects for recovery in it environment. This study is part of a LIFE project consisXng of a populaXon reinforcement of this species through capXve breeding, release and follow-up
populaXon to see the different stages of the process of adaptaXon to the new environment. The current work studies releases of two consecuXve years: the release made in 2015 to see the growth of the populaXon and if they conXnued
adapXng to the environment and the release of this year to closely monitor the movements that they made for exploraXon and adaptaXon to the new territory. Subsequently, the data of these two years will be used to complete the project in 2017 with new insights into the biology of this turtle that will enable us to choose the best environments to enhance future populaXon reinforcement. This year newly created ponds, a canal in Sobrànigues and another pond in Flaçà were selected for the release of 50 individuals. Monitoring is done with a system of transmimers and one
receiver and to conXnue to monitor the released in 2015, a trap system is used in order to give us informaXon about their growth and populaXon. Kernel index was calculated to determine the home range, the core area and fidelity of specimen. Only one individual remained in its area, the rest keep on seeking an opXmal environment. Catches have
shown no significant differences between areas or between the sexes in terms of growth and that populaXons are stable as far as the number of individuals is concerned
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Biologia (TFG)
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Emídids Cria i desenvolupament
Emídids Protecció
Emydidae -- Conservation
Emydidae -- Breeding
Biología
Projecte de reforçament poblacional d'Emys orbicularis a les riberes del Baix Ter
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/130392021-05-17T06:48:26Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Osuna Oliveras, Sílvia
Estévez Gay, Miquel
Universitat de Girona. Facultat de Ciències
2016-11-04T11:55:54Z
2016-11-04T11:55:54Z
2016-09
http://hdl.handle.net/10256/13039
Enzymes are the best catalysts known, they allow increasing the rate of a chemical reaction and do it under mild conditions of pressure, temperature and pH suitable for life. With this awareness, there have been a lot of directed evolution studies for the improvement or modification of enzymes for improved production of products such as pharmaceuticals. These strategies require a lot of work, money and time but enzymes obtained are very active. However, they have the disadvantage of not being rational. Moreover, improved protocols for enzyme design completely based on computations, such as the Inside-Out protocol, can also be performed. These are much cheaper and faster, and have the advantage of allowing the rational design of enzymes, but have the limitation of generating variants whose activity is low compared to the directed evolution-based ones. This work focuses on the enzyme haloalcohol dehalogenase (HheC). This enzyme performs the dehalogenation reaction of toxic compounds but also has the ability to catalyze the reverse reaction. This second reaction is very interesting because it allows the synthesis of a precursor of drugs called atorvastatin (Lipitor). Fox et al conducted a directed evolution experiment for haloalcohol dehalogenase which resulted in a variant with 38 mutations and 4000 times more effective for the mentioned reaction of great importance. The data generated in this experiment, and data from Schallmey and collaborators, who created a more efficient variant of the wild-Miquel Estévez Gay
type for the reaction previously discussed only by varying two positions, have served as a basis for understanding which is the effect of the mutations introduced in these proteins along the directed evolution pathway. Thanks to this work, have drawn conclusions of great importance for the future improvement of computational protocols for enzyme design, all using simulations of molecular dynamics (MD), which simulate the generated proteins and their ligands in aqueous medium, and analysing the data generated by computational chemistry tools
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Enzims
Dinàmica molecular
Proteïnes Conformació
Enzymes
Molecular dynamics
Proteins -- Conformation
Biología
Molecular Dynamics Simulations of experimentally-evolved halohydrin dehalogenase enzymes for the synthesis of Lipitor
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oai:dugi-doc.udg.edu:10256/130462016-11-10T10:14:31Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vila i Gispert, Anna
Cuello Viñolas, Aida
Universitat de Girona. Facultat de Ciències
2016-11-04T13:19:57Z
2016-11-04T13:19:57Z
2016-09
http://hdl.handle.net/10256/13046
The hidrological intermittence is a common perturbation in Mediterranean rivers which dry out during summer forming isolated puddles between them. The construction of dams can cause that a river, which previously had a permanent flow, becomes intermittent in some of its sections. This type of perturbations cause changes in the communities which live associated with the aquatic ecosystems. Macroinvertebrates are important indicators of the condition of these ecosystems because they are really sensitive to perturbations. Modifications of macroinvertebrates communities can cause several changes in the predator fish’s diet, which can see themselves obligated to feed on less nutritious preys. In order to evaluate this effect, macroinvertebrates comunities and the diet of the mountain barb (Barbus meridionalis) have been studied in two sections separated by a dam that causes intermittent hydrological periods down the Llémena stream. Several differences have been found in biomass and abundance of the macroinvertebrates communities and in the abundance of the mountain barb in both sections, but differences haven’t been found in the composition of its diet. This study demonstrates that the presence of the dam has a clear effect on the macroinvertebrates community as well as to the density of mountain barbs. However, the barb’s diet is not affected because it feeds mainly on chironomidae larvae, which were really abundant in both sections
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Fauna dels corrents d'aigua
Invertebrats aquàtics
Stream fauna
Aquatic invertebrates
Fishes -- Climatic factors
Peixos -- Factors climàtics
Biología
Efectes de l’alteració hidrològica en les comunitats de macroinvertebrats i en la dieta de les poblacions de barb de muntanya en la Llémena
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oai:dugi-doc.udg.edu:10256/130472019-09-27T05:57:29Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Massaguer i Vall-llovera, Anna
Marquez Dorca, Olga
Universitat de Girona. Facultat de Ciències
2016-11-04T13:50:03Z
2016-11-04T13:50:03Z
2016-09
http://hdl.handle.net/10256/13047
Lung cancer is one of the causes of death around the world, because of its aggressiveness in advanced stages, so investigators proposed early detection and prevention of this disease.
Currently, one of the techniques used to tumor detection is the low dose computed tomography, but it has some limitations, which is why they are looking for alternatives that can complement the diagnostic.
The objective is to reach a kit of biomarkers associated with lung cancer that can be diagnosed in peripheral blood. This detection could be realized with conventional PCR or real-time PCR, it could determine a low cost technique, could perform the test with relatively short time and there was the possibility of repeating without impact on the patient. The possibility of achieving a method with all of these advantages is what makes it so attractive alternative.
So far many research groups have been studying this possibility and they found several regions of the genome in peripheral blood related to lung cancer. Epigenetic changes in DNA (aberrant DNA methylation and microRNA) are leading the molecular markers studied in peripheral blood of Caucasian patients with lung cancer.
The aberrant methylation in certain loci has been implicated in carcinogenesis. This may affect genes involved in cell cycle control, DNA repair, cell adhesion, signal transduction, apoptosis and differentiation. MicroRNAs also have been involved in lung cancer, and represent non-coding part of the genome; these molecules may alter the expression of certain genes.
The objective of this strategy is to reach sets of biomarkers that display a high sensitivity and specificity, which have high effectiveness in diagnosing this disease in an early stage
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Pulmons -- Càncer
ADN -- Metilació
DNA -- Methylation
Lungs -- Cancer
Biología
Càncer de pulmó, una revisió sobre el coneixement actual i perspectives del diagnóstic genètic
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/144132017-10-19T10:15:46Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Cebrian Pujol, Emma
Vinagre, Catarina
Olmo Arjona, Marina del
Universitat de Girona. Facultat de Ciències
2017-10-08T08:55:38Z
2017-10-08T08:55:38Z
2017-06
http://hdl.handle.net/10256/14413
The intertidal zone of the temperate Portuguese coasts is a species rich habitat with complex communities, which is a product of a convergence of physical factors - such as the effect of waves and tides - and biological- interactions between individuals and between individuals and environment - that make these intertidal communities a characteristic ecosystem of great ecological value and of scientific interest.
The present study aims to study the seasonal variations of biodiversity in two different areas located in the beach of Cabo Raso, in Cascais (Portugal). These are: Cabo Raso 1 and Cabo Raso 2 (figure 1). For this purpose, samples of a transept of thirteen meters were collected on both places and a counting, measurement and identification of the individuals and algae present in the samples was done. Based on the data obtained, the diversity indexes of Margalef, Pielou, Fisher, Brillouin, Shannon-Wiener, Simpson, Hill and rarefaction index were calculated along with species richness and number of individuals using PRIMER 6 software. The relative abundances of the species of individuals in each season of the year and in each area were also calculated in order to determine the most abundant species of macroinvertebrates and algae.
The results of the indexes showed seasonal diversity patterns in both areas, with maximum biodiversity in summer for Cabo Raso 1 and 2 and minimum biodiversity during autumn and spring on the area of Cabo Raso 1 and Cabo Raso 2 respectively. Biodiversity values were higher in Cabo Raso 1. The dominant macroinvertebrate species at Cabo Raso 1 were Mytilus galloprovincialis, Rissoidae sp. Fabricia sterallis and M. galloprovincialis in autumn, winter, spring and summer, respectively. Corallina elongate was shown as the most abundant species of algae in Cabo Raso 1. At Cabo Raso 2, Melarphaphe neritoides, M. galloprovincialis, Cthamallus sp. and Chironomidae were found as major species in the seasons of autumn, winter, spring and summer respectively. The majority species of macroalgae in Cabo Raso 2 were Fucus spiralis in autumn and winter, Codium sp during spring and Corallina elongata in summer.
The realization of this study acknowledge the seasonal diversity of two characteristic areas within a characteristic beach of the intertidal zone, in parallel with the characterization of the community of macroinvertebrates and algae during the four seasons
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Invertebrats aquàtics
Algues marines
Aquatic invertebrates
Marine algae
Biología
Biodiversity seasonal patterns of marine benthic habitats of the Portuguese coast
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
east=-9.48694439999997; north=38.71; name=Cabo Raso (Portugal)
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oai:dugi-doc.udg.edu:10256/144142017-10-19T10:16:44Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Hernández-del Amo, Elena
Ramis Jorba, Gal·la
Universitat de Girona. Facultat de Ciències
2017-10-08T09:05:36Z
2017-10-08T09:05:36Z
2017-06
http://hdl.handle.net/10256/14414
The first step of nitrification, oxidation of ammonia to nitrite, is performed by both ammonia-oxidising archaea (AOA) and ammonia-oxidising bacteria (AOB). Oxidation of ammonia in water and sediments is highly dependent on the availability oxygen. Emergent macrophytes, such as Typha angustifolia, increase oxygen concentrations in the rhizoplane due to continuous oxygen leakage from roots, thus generating an aerobic microenvironment in close contact to the root surface
The abundance, diversity and community structure of AOA and AOB were investigated, using amoA and 16S rRNA genes as a molecular biomarkers, to examine how oxygen permeability affect the spatial distribution of AOA and AOB on the root surface of Typha angustifolia, of three aquatic systems with different water conductivity. Massive sequencing and qPCR results showed that AOBs were the predominant ammonium oxidants in all three systems. The main AOB was Nitrosococcus and the main phylum of AOA was Thaumarchaeota. The relative abundances of the amoA gene in bacteria and archaea were significantly different in the three aquatic systems analysed. Likewise, comparisons of different aquatic systems with the 16S rRNA biomarker showed a heterogeneous distribution of the community. The different sections of the root (base, medium, tip, sediment) showed a homogeneous distribution of the microbial community but a significant difference of alpha-diversity.
Overall, we were able to show that the communities of ammonium oxidizers linked to the roots of Typha angustifolia are mainly affected by the physicochemical conditions of the system, while the effect of oxygen diffusion at different parts of the root affects less
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Cicle del nitrogen
Amoni
Flora dels corrents d'aigua
Nitrogen -- Fixació
Nitrogen cycle
Ammonium
Stream plants
Nitrogen -- Fixation
Biología
Caracterització de la comunitat de bacteris i arqueus oxidadors d’amoni a arrels de Typha angustifolia en diferents sistemes aquàtics
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
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oai:dugi-doc.udg.edu:10256/144152017-10-19T10:17:43Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Casadevall, Margarida
Palacios Sargatal, Nina
Universitat de Girona. Facultat de Ciències
2017-10-08T09:19:11Z
2017-10-08T09:19:11Z
2017-06
http://hdl.handle.net/10256/14415
The teleost and benthic fish studied, the Serranus cabrilla, colloquially called serrano, is a functional simultaneous hermaphrodite fish and presents ovotestis. During the breeding period produce and mature sperm and eggs simultaneously. It is the only genus that presents this type of hermaphroditism in the Mediterranean.
The objective of this study is to provide new information about the reproductive strategy of Serranus cabrilla as spawning season, fecundity and if the self-fertilize likely or not.
It was collected during April and May 2016 in different parts of the Costa Brava; its total length varied between 147mm and 233mm, and the standard length varied between 116mm and 194mm. The methodology used was: histological observation of the gonads, calculation of indices related to reproduction and finally evaluation of fecundity.
Testicular tissue occupies less space within the gonad compared to ovarian tissue, which has an asynchronous development: oocytes coexist in different stages of development. From the HSI has not been observed that there is a remarkable accumulation of lipids in the liver previous to oocyte maturation.
Testicular tissue begins to mature in February and ovarian tissue during the month of March, when we begin to observe vitellogenic oocytes with a greater diameter than 200μm. The spawning season of the Serranus cabrilla is in May and June, since we are observed hydrated oocytes and postovulatory follicles. In addition there is an increase of the GSI between April and May and then stabilizes in June, corroborating the beginning and continuation of the spawning between May and June. The spawning of Serranus cabrilla is asynchronous. Mature and viable oocytes released into the medium have a diameter greater than 500μm. The average fecundity of the Serranus cabrilla is 6.445 eggs during the spawning season where the range varies between 1.200 eggs to 22.072 eggs and the potential fecundity is of 10.551 eggs.
Self-fertilization in Serranus cabrilla is unlikely; everything seems to indicate that they release the gametes sequentially and only when they have found an individual with whom to mate
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application/pdf
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Perciformes -- Reproducció
Perciformes -- Reproduction
Biología
Estudi de la fecunditat i estratègia reproductiva de Serranus Cabrilla (Pisces, Serranidae)
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
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oai:dugi-doc.udg.edu:10256/144162018-11-06T14:36:32Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Sancho Badell, Sílvia
Sola Idígora, Noelia
Universitat de Girona. Facultat de Ciències
2017-10-08T09:49:40Z
2017-10-08T09:49:40Z
2017-06
http://hdl.handle.net/10256/14416
According to the Spanish Fertility Society (SEF) among 15-17% of the population of reproductive age have troubles having a baby. The causes of infertility may lie in the man, the woman, in both members of the couple or may be idiopathic.
In order to give solution to fertility problems assisted reproductive techniques (ART) were developed. Such techniques involve the handling of oocytes or sperm in a laboratory. The ART, however, does not provide a solution to all cases of sterility. Currently the most commonly ART used are artificial insemination (AI), in vitro fertilization (IVF) and intracytoplasmatic sperm injection (ICSI), which is derived from IVF. To successfully perform these techniques, most patients undergo controlled ovarian hyperstimulation (COH) protocols. The COH has the purpose of stimulating the ovaries through hormonal treatments to obtain more than a mature follicle and to increase in this way the probabilities of pregnancy. There are several protocols available to develop these techniques, being the gonadotropins the most used treatment because of their intense stimulation. But if mild stimulations is desired clomiphene citrate may be an alternative. In addition, there is also the possibility of using an adjuvant treatment by using gonadotropin-releasing hormone (GnRH) analogues, both agonists and antagonist, which significantly increase success rates due to an improvement in oocyte quality. Complications and adverse effects such as oocyte abnormalities, problems in endometrial receptivity, or two of the most recurrent complications like multiple gestation and ovarian hyperstimulation syndrome (OHSS) can also arise from COH. There are a numerous protocols available to be followed, and each one derives advantages and disadvantages, so there is a need to individualize the technique to each patient considering age, ovarian reserve and medical history
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Attribution-NonCommercial-NoDerivs 3.0 Spain
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Reproducció humana assistida
Human reproductive technology (general)
Fecundació in vitro
Inseminació artificial humana
Fertilization in vitro
Artificial insemination, Human
Biología
Hiperestimulació ovàrica i tècniques de manipulació d’oòcits en el tractament de la infertilitat humana
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/144572018-04-04T08:05:45Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
García Marín, José Luis
Amer Cid, Albert
Universitat de Girona. Facultat de Ciències
2017-10-19T08:06:34Z
2017-10-19T08:06:34Z
2017-09
http://hdl.handle.net/10256/14457
Since the past century, the brown trout populations (Salmo trutta) of the Catalan Prynne's rivers have suffered from large restocking of trouts, with the aim of maintaining or improving the natural river's populations and to favor the sport fishing activity, with hatchery-reared non-native fish from Central Europe. These are genetically different from the native Mediterranean populations, where both groups represent the two groups represent divergent evolutionary lineages. Such measure have become a serious threat to the conservation of the native genetic diversity due to genetic introgression processes. In order to change this situation, improve the genetic state and to prevent futures hybridizations, brown trout genetic refuges were created in several sections of the rivers of the different basins. Between the period of 2014 - 2016, the Autonomous Catalan Government restarted the monitoring of the genetic estate of the wild populations of S. trutta, previously done between 1993 until 2006, in order to actualize the information about their genetic state. Two genetic markers were used, a group of 5 microsatellites and the locus LDH-C1*, as to evaluate the average genetic introgression degree for each section of the rivers sampled. In the present project we created a data base (with a total of 73 samples) with the genetic information collected during this 2014 - 2016 period in the basins of the Ter, Llobregat, Segre, Noguera Pallaresa i Noguera Ribagorçana, and was determined for every sampled point if it was included or not in a genetic refugee at the moment of the extraction. After the analysis of the such data, we found the existence of significant differences for the genetic introgression degree between the different Catalan Pyrenees basins. But differences on the mean introgression degree between analyzed river stretches inside and outside of the genetic refuges were not found. The expansion and creation of new genetic reserves will be essential for an improvement in the conservation of the native genetics populations. We propose the extension of the genetic reserve of N. Pallaresa to include all the sites analyzed in this project and the extension of the genetic reserve of the Segre basin for the Oden River, due to its exceptional low level of introgression
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Genètica de poblacions -- Catalunya -- Pirineu Català
Truites (Peixos) -- Genètica -- Catalunya -- Pirineu Català
Truita comuna -- Catalunya -- Pirineu Català
Population genetics -- Catalonia -- Pyrenees
Trout -- Genetics -- Catalonia -- Pyrenees
Biología
Estatus de les poblacions de truita comuna al Pirineu
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/146672021-10-04T08:48:27Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Campos i Llach, Miquel
Berloso Clarà, Ferran
Universitat de Girona. Facultat de Ciències
2017-11-30T08:41:17Z
2017-11-30T08:41:17Z
2017-09
http://hdl.handle.net/10256/14667
The long freshwater mussel (Unio mancus, Lamarck 1819) is a threatened mollusc in Catalonia. Because of this, and taking part of the LIFE Potamo Fauna project, the “Consorci de l’Estany” creates a freshwater mussel’s breeding laboratory, where this specie is breeded in captivity in order to carry out reintroductions and population reinforcements.
The aim of this study is make improvements on the in vitro culture, a technique which allows the breeding in captivity of this specie without the use of a host fish. The glochidia will be cultured in a medium with 3 essential components; M199 medium, rabbit or carp serum and a mixture of antibiotics and antimicotics. In addition, another goal of this study is to prevent the fungal contamination caused by oomicets, because of this, malachite green will be used. The glochidia will be cultured in an incubator controlling the temperature and the CO2 %, fact that allows a greater pH stability. Besides that, it is pretended to study the in vitro protocol and to make improvements on it, so two techniques are proposed here: the use of vials or the use of petri dishes. The goal here is to figure out which one is the best. Every day a change of the 50% of medium will be done, and the fungal contamination will be removed. A monitoring of glochidia is done after few days to see how they develop.
The results of this study indicate on one hand that malachite green has a significant effect on the oomycete contamination and it could be used to remove that contamination. On the other hand, the glochidia did not complete their metamorphosis and most of them died, so some aspect of the culture has failed (precariousness medium, contamination of mediums, extended exhibition of glochidia to clean medium MEM, or overdose of antibiotics or antimycotic). The methodology with the best results is the use of vials, with more advantages than the use of petri dishes. The advantages are, for instance, less contamination, less dirty in the medium, more pH stability and more facility when manipulating the samples. For this reasons, it is proposed for future studies the use of vials, and the implementation of some improvements, such as the use of vials with porous top or the less exhibition of glochidia to MEM medium
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Nàiades (Mol·luscos) -- Reproducció
Nàiades (Mol·luscos) -- Cultiu in vitro
Freshwater mussels -- Reproduction
Freshwater mussels -- Micropropagation
Biología
Millora de procediments de reproducció ex situ de nàiades
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/146682017-11-30T08:44:44Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Blanch Ojea, Estel
Universitat de Girona. Facultat de Ciències
2017-11-30T08:44:44Z
2017-11-30T08:44:44Z
2017-09
http://hdl.handle.net/10256/14668
Nowadays society is trying to approach citizens to the wildness that surrounds the cities. A few years ago, in Girona was born the project “la Vora de Girona” that aims to create a green circle around the city. One of those areas is Torre Gironella, provided with a diversity of wild animals. This thesis aims to determinate the composition and distribution of this fauna, specifically, terrestrial vertebrates (amphibians, reptiles, birds and mammals) which are good indicators of habitat status, providing information in order to evaluate the effect of the management.
The sampling was carried out from the 27th of February until the 4th of May 2017, and consisted of a combination of six methodologies applied to the different areas of study: unmanaged forest (FNG), managed forest (FG), managed meadow (PG), deciduous forest (BC), and shrubland (BR). The live-trapping method allowed the study of the small mammals, while the photographic trapping and the indirect census allowed the detection of the medium and large mammals. To monitor birds and reptiles, the census was carried out through a transect that covered the five studied environments. Finally, three hearing points were established in the Galligants River for the auditive study of the amphibians.
The study allowed the detection of 9 mammals, 24 birds, 2 reptiles and 2 amphibians. Unfortunately, there was no clear result of the effect in the management of the project in the fauna. Nevertheless, through the observation of the distribution, the selection made by the species to find the most optimum habitat was demonstrated. The micromammals are found in unmanaged forest (FNG), since this area is provided with kermes oak, which provides shelter and food. In the case of birds, there have been more species associated to forest areas than to open, urban or general areas. Reptiles, however, prefer open spaces with rocks, where it is easier for them to heat themselves up. Finally, as only one single species of amphibian has been found it means that there is a lack of optimal water points for their reproduction. It was also possible to observe how the daily activity of wild mammals coincides with that of cats, which generate strong pressure on the former, both by the predation of their descendants and the competition for space and food with medium and large mammals. One aspect that was also clearly seen was the effect of human activity on wildlife
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Fauna -- Catalunya -- Girona
Animals urbans -- Catalunya -- Girona
Urban animals -- Catalonia -- Girona
Fauna -- Catalonia -- Girona
Biología
Fauna vertebrada de la zona periurbana de Torre Gironella: caracterització i efectes del projecte de la vora de Girona
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
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2017-11-30 09:44:44.657
DUGiDocs
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oai:dugi-doc.udg.edu:10256/146692017-11-30T08:48:53Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Zamora Hernández, Lluís
Conesa Rubio, Marc
Universitat de Girona. Facultat de Ciències
2017-11-30T08:48:53Z
2017-11-30T08:48:53Z
2017-09
http://hdl.handle.net/10256/14669
The aim of this project is study the otter (Lutra lutra) population of the lake System of Banyoles, which is not well known. To understand this population dynamics, the activity pattern, diet, distribution and habitat use of the otter were examined. A total of 59 otter spraints were collected, 93 sites were recorded across the habitat and 25 sites were monitored using remote cameras.
The diet composition of otters in the study zone was investigated by spraint analysis between summers of 2006, 2008 and 2017, and also with the results obtained by Clavero et al., 2003 in Mediterranean freshwater habitats, using the percentage frequency of occurrence. Overall, American crayfish (Procambarus clarkii) represented the dominant food, followed by fish and amphibians. The actual food composition of otters differed significantly from 2006 and 2008 composition and those from Mediterranean freshwater habitats, increasing the consumption of crayfish and becoming the main food resource in the summer period. In addition, Shannon trophic diversity index was calculated and demonstrated a narrow niche breadth.
The distribution and habitat use map was carried out form data of the 93 recorded sites, and we located two focuses of the otter activity. Significant correlations were recorded between otter activity and vegetation cover, road and city proximity. The activity pattern was calculated from captures of the camera traps, deployed in riparian habitat along streams and lakeshore. We found that otter population of the study area haven’t modified their activity pattern from otters of the Iberian Peninsula.
From the results, it follows that a conservation plan for the study zone should suggest an improvement of the riparian habitat in those sections that could be used as corridors for the movement of the individuals connecting the different populations (or subpopulations), like the channels and the Canaleta brook. Likewise, it’s important to undertake actions like the recovery and conservation of the natural space of Can Morgat forest, which is the centre of otter population activity
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Previous issue date: 2017-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Llúdries -- Catalunya -- Banyoles, Estany de
Otters -- Catalonia -- Banyoles, Estany de
Biología
Anàlisi de la dieta i rang de distribució de la llúdriga (Lutra lutra) a la zona lacustre de l’estany de Banyoles
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
east=2.756825899999967; north=42.13043419999999; name=Estany de Banyoles
NO
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ConesaRubio_Marc.pdf
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2017-11-30 09:48:53.204
DUGiDocs
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oai:dugi-doc.udg.edu:10256/146702018-04-04T08:10:38Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Romaní i Cornet, Anna M.
Guasch i Padró, Helena
García Ibáñez, Miguel
Universitat de Girona. Facultat de Ciències
2017-11-30T08:54:32Z
2017-11-30T08:54:32Z
2017-09
http://hdl.handle.net/10256/14670
The main objective of this study was to determine the effect of Fe in Font de Ferro’s water on the leaf litter decomposition mostly from Quercus pubescens. The field work was performed between December 2016 and January 2017, in each sampling a bag of oak leaves was collected from each point as well as pH, conductivity, dissolved O2, temperature and water velocity data. In the laboratory leaf weight loss, alkalinity, absorbance, fluorescence index, metal content (Al, B, Fe, Mn, Rb, Sr, Cr, Co, Ni, Cu, Zn, As, Sn, Sb i Ba), nutrient content (NH4+, PO43-, P org, Cl-, NO2-, NO3- and SO42-), observation of hydromycetes diversity and concentration of ergosterol (to know fungal biomass) were measured. The decomposition rates varied (points 1-4) from -0,0021 to -0,0234 days-1, and this variation fitted with the Fe concentrations in the water going (points 1-4) from 4972,53 to 78,64 ppb, also with water conductivity values varying from 6,71 to 6,11 mS/cm and with ergosterol values (from 6,65 to 56,75 μg ergosterol/g dry weight between points 1-4). The 5th point results follow a completely different trend from the rest of points. Statistical analyses were ANCOVA to observe if the evolution of the percentage of organic matter remaining varies significantly depending on the days that the leaf bags in the water have passed and the distance of the sampling point from the source, a Tukey test to observe if there were significant differences between the decomposition rates of the different points and 2 PCA’s (one with physicochemical variables and Fe, and another with metals, pH and dissolved O2) to analyse the variability of the data in function of two new variables that explained about 60% of the variability. These results suggest that Fe affects the rate of decomposition and allow the division of the source in 3 parts: 1st (points 1-2) where the Fe concentration is high, the massive Fe precipitation and high water conductivity inhibited the decomposition and photosynthesis; 2nd (points 3-4) where these inhibitory factors did not act; and 3rd (point 5) where all tendencies were broken because the water passed under the sediments and this changed their characteristics
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Font de Can Verdaguer (St. Gregori, Gironès) -- Aspectes ambientals
Fullaraca -- Biodegradació
Water -- Analysis -- Catalonia -- Font de Can Verdaguer (St. Gregori, Gironès)
Font de Can Verdaguer (St. Gregori, Gironès) -- - Environmental aspects
Aigua -- Anàlisi -- Catalunya -- Font de Can Verdaguer (St. Gregori, Gironès)
Plant litter -- Biodegradation
Biología
Estudi de la descomposició de la fullaraca i de la fisicoquímica de l’aigua de la Font de Ferro de Can Verdaguer
info:eu-repo/semantics/bachelorThesis
east=2.732143599999972; north=41.99547039999999; name=Sant Gregori
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oai:dugi-doc.udg.edu:10256/146712017-11-30T08:59:08Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Cebrian Pujol, Emma
González Sampedro, Yolanda
Universitat de Girona. Facultat de Ciències
2017-11-30T08:59:08Z
2017-11-30T08:59:08Z
2017-09
http://hdl.handle.net/10256/14671
The Mediterranean Sea is a hotspot of marine species biodiversity where most of the submerged forests are dominated by fucoid species of the genus Cystoseira. Benthic assemblages subjected to a low or moderate hydrodynamics between zero and one meter deep and restricted to rocky subtracts are occasionally dominated by Cystoseira crinita, that because of the shallow habitat is particularly vulnerable and finds refuge in rock pools and in very shallow waters of sheltered coves.
The most important consequences of climate change on aquatic ecosystems are probably the temperature and UV radiation increases. These rocks pools, due to the low deep and hydrodynamics may experience high temperatures and radiations that, especially considering the present climate warming framework, may impair these populations which are actually vulnerable.
The main objective of this study is to determine if positive thermic anomalies may be a driver of C. crinita decline, at least in the north-western Mediterranean Sea. For this, our specific aims are: determine if the temperature and ultraviolet radiation increases affects physiological state of adults individuals and elucidate if temperature and radiation have a synergistic, cumulative or antagonistic effect on this physiological state.
The experiment consisted on the application of six treatments combining two different environmental parameters, radiation (PAR and PAR + UV) and temperature (21, 24 and 28 ºC) during 30 days. At 21 and 24 ºC, in both radiations, total height and maximum quantum yields of photosynthesis, Fv/Fm, remained constant during experiment, but humid weight began to decrease after 30 days and the Fv/Fm after 12. At 28 ºC effects were differentially caused by radiation exposure, so that under PAR began to lose humid weight after day 12 and reduced its total height, but under PAR + UV began to lose humid weight after day 18. In addition, at 21 ºC under PAR + UV radiation individuals increased its apex height.
These results suggest that C. crinita has efficient protective mechanisms against UV radiation and its thermic tolerance is adapted to resist a long time under the current normal summer temperatures. However, our results also show that although C. crinita is able to resist short and punctual periods at high temperatures, it is not able to resist mid-long periods at temperatures up to 28ºC (thermal anomalies). Also, the interaction between temperature and radiation has an antagonistic effect
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application/pdf
eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Cistoceiràcies -- Mediterrània, Mar
Cistoceiràcies -- Efecte de la temperatura
Cistoceiràcies -- Efecte de la radiació
Cystoseiraceae -- Mediterranean Sea
Cystoseiraceae -- Effect of temperature on
Cystoseiraceae -- Effect of radiation on
Biología
The Challenge of Cystoseira crinita: combined effect of temperature and radiation
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
east=18.048010500000032; north=34.5531284; name=Mar Mediterrània
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oai:dugi-doc.udg.edu:10256/146722017-11-30T09:02:03Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vila i Gispert, Anna
Martínez Ardenuy, Òscar
Universitat de Girona. Facultat de Ciències
2017-11-30T09:02:03Z
2017-11-30T09:02:03Z
2017-09
http://hdl.handle.net/10256/14672
The bleak (Alburnus alburnus) (L.) is a cyprinid specie that can inhabit both lotic and lentic environments. They follow a generalist diet, since they can feed on multiple dams preys: from terrestrial insects to zooplankton, algae or detritivores. This specie, native to most of Europe, was introduced to the Iberian Peninsula during the 90's, where it has expanded and become invasive.
The main objective of this study is to determine the trophic ecology of a native population of alburn based on the characterization of the diet through different trophic parameters (prey richness, Shannon’s index and trophic niche width) and with Fulton's physical condition index.
It is also analyzed whether there are differences between the trophic parameters based on sex. Finally, Finally, different trophic parameters are compared between this native population and different introduced populations
The specimens were captured with electric fishing during the spring of 2014. They were later processed in the laboratory, obtaining the following parameters: furcal length (FL), eviscerated weight, sex and size structure. All the procedures carried out during the sampling and the laboratory work comply with the regulations for animal welfare and use.
The obtained results suggest that the size structure of the population in males and females follows a unimodal pattern, indicating that we find only one cohort.
Ingested and occurrence biomass percentages show a lesser diversity in the diet compared to invasive populations, and reveals that one of the secrets of the success of the bleak is its opportunism according to the conditions of its habitat.
Statistically no significant differences are observed in any of the diet-related parameters between the sexes, but they are noticed in their condition. Males have a better physical condition than females because the sampling was performed after the egg-laying period
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Ciprínids -- Alimentació
Cyprinidae -- Feeding and feeds
Biología
Ecologia tròfica d’una població autòctona d ’alburn (Alburnus alburnus)
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
NO
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oai:dugi-doc.udg.edu:10256/146732017-11-30T09:06:00Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pons Ferran, Pere
Miarons Carbasa, Carla
Universitat de Girona. Facultat de Ciències
2017-11-30T09:06:00Z
2017-11-30T09:06:00Z
2017-09
http://hdl.handle.net/10256/14673
Wildfires are one of the most important disturbances that worry the society, efect the forests that everyday
where more common. This had generated that the last years so much progress has been made in the study
of forest management after a wildfire and the effects that this has to the biodiversity and the structure of
de comunities of the forests. Ants are a group of species with an important ecological function in the
different ecosystems and they can be bioindicators of disturbances and effects that had happened in the
forests. So in this study we use ants, diversity and the structure of comunities to evaluate the impacts of the
forest management. The sampling was done in Blanes in a wildfire of summer 2016 and the salvage logging
during the spring of 2017, where we took samples of the ants and we do the statics analysis. This is taken
about see the diferent functional groups of the species that we have in the sampling, the position in the
food web, the abundance and the species richness. The sampling has been done with different treatments
and microhabitats in the forest. After the analysis, we don't see importants diferences between the diferent
tractments surely because the management was done a little bit time after the sampling
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Formigues -- Efecte dels incendis
Ecologia del foc -- Catalunya -- Blanes
Ants -- Effect of fires on
Fire ecology -- Catalonia -- Blanes
Biología
Impacte immediat de la gestió de boscos cremats en la comunitat de formigues
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
east=2.77701; north=41.71438; name=Mare de Déu del Vilar (Blanes)
NO
LICENSE
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MiaronsCarbasa.Carla.pdf
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oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/146742018-04-04T08:10:10Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bussalleu Muntada, Eva
Palahí Oller, Laura
Universitat de Girona. Facultat de Ciències
2017-11-30T09:12:14Z
2017-11-30T09:12:14Z
2017-09
http://hdl.handle.net/10256/14674
Cryopreserved semen is very useful for artificitial inemination (AI) since it enables the semen to be stored for unlimited period of time ranging from months to years until it needs to be used. However, in the swine industry only 1% of the AIs are conducted with cryopreserved semen due to the low fertility rate of thawed semen samples. One of the factors that affect directly the quality and storage of sperm is, among others, bacterial contamination, referred as bacteriospermia. Bacteria can have detrimental effects upon the sperm cell causing, among others: sperm agglutination, damaged acrosomes, premature acrosome reaction and low motility and viability rates. Moreover, if the bacteria are pathogenic there is a risk of passing a disease to the sow. Bacteriospermia, is an issue that has been studied among mammals with fresh and cryopreserved semen. Nonetheless, with boar up to this date no articles have been found addressing bacterial contamination in cryopreserved (or frozen-thawed) semen samples. In this study an evaluation of bacteriospermia after sperm thawing. The semen samples belonged to four different boars and they had been cryopreserved in liquid nitrogen (LN2) for three years inside sealed plastic straws. Four straws, each from a boar were assessed for bacterial contamination. A total of sixteen colonies were isolated, which a total of thirteen out of sixteen (81.25%) could be identified by using the Polymerase chain reaction (PCR) and the BBL™ Crystal™ Enteric/Nonfermenter kit. The results showed that contamination occurred in all samples, being Gram-negative bacteria (68.75%) more prevalent. The contaminants isolated were Staphylococcus spp. (18.75%), Streptococcus spp. (12.5%), Burkholderia cepacia (25%), Yersinia pseudotuberculosis (6.25%), Yersinia enterocolitica (6.25%), Pasturella aerogenes (6.25%) and yeast (6.25%). Thus seems to show that bacteria present in semen samples are able to withstand the cryopreservation process. However, it cannot be determined which source of contamination might be, and it can only be hypothesised by literary review
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Bacteriologia veterinària
Veterinary bacteriology
Porcs -- Espermatozoides -- Investigació
Porcs -- Inseminació artificial
Porcs -- Recursos germoplasmàtics -- Crioconservació
Crioconservació d'òrgans, teixits, etc.
Swine -- Artificial insemination
Swine -- Spermatozoa -- Research
Cryopreservation of organs, tissues, etc.
Swine -- Germplasm resources -- Cryopreservation
Porcs -- Espermatozoides -- Anàlisi
Swine -- Spermatozoa -- Analysis
Biología
Evaluation of bacteriospermia after the cryopreservation process of boar semen samples= avaluació de la bacteriospèrmia després del procés de criopreservació de semen de porc
info:eu-repo/semantics/bachelorThesis
NO
LICENSE
license.txt
license.txt
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2018-04-04 10:10:10.357
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oai:dugi-doc.udg.edu:10256/146752017-11-30T09:18:04Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
García Marín, José Luis
Xargay i Torrent, Sílvia
Roca Molas, Anna
Universitat de Girona. Facultat de Ciències
2017-11-30T09:18:04Z
2017-11-30T09:18:04Z
2017-09
http://hdl.handle.net/10256/14675
Epigenetics has been involved in the relationship between maternal overnutrition and fetal development. The placenta is a transitory tissue that regulates the endocrinometabolic functions and development of the fetus. Imprinted genes have an important role in the function of the placenta and fetal growth, and are regulated through DNA methylation. Among the imprinted genes in placenta there are the gene clusters DLK1-DIO3 and C19MC. In this context, the objectives of the study were: (I) to study the DNA methylation of DLK1-DIO3 and C19MC in placenta; and (II) to study the association between the methylation of these genes and the anthropometric and endocrinometabolic parameters of pregnant women and their respective children.
In order to achieve the objectives, a cohort of 60 pairs of pregnant woman-child from the region of Alt Empordà was studied with available clinical data. DNA was extracted from the placenta, bisulfited and the region of interest was amplified with specific primers for bisulfited DNA. The obtained product was pyrosequenced to quantify the percentage of methylation of each CpG. The results were statistically analyzed with SPSS software and significance level was set at p=0.05.
No significant differences were found regarding the methylation profile of the studied regions of DLK1-DIO3 and C19MC when comparing the 4 study groups (control (1) group compared with (2) pregestational with gestational obesity, (3) pregestational obesity and (4) gestational obesity). However, the percentage of methylation of DLK1-DIO3 positively correlated with maternal blood pressure and glycated hemoglobin (HbA1C), whereas the methylation in C19MC was associated with maternal insulin, triglycerides and total cholesterol. Regarding their respective children, no significant relationships were found. After correcting for multiple testing, total maternal cholesterol was still associated with the methylation level of C19MC (r=0.624; p=0.004). In a multivariate linear regression test, maternal total cholesterol correlated independently with the C19MC methylation percentage, explaining a 43% of the variance (β=0.682; p=0.001).
In conclusion, maternal total cholesterol and the methylation level of C19MC in placenta are related, and as a consequence they could affect fetal endocrinometabolic development
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Biologia (TFG)
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info:eu-repo/semantics/openAccess
Embaràs -- Aspectes nutricionals
Epigenètica
Genètica mèdica
Pregnancy -- Nutritional aspects
Medical genetics
Epigenetics
Biología
Marques epigenètiques del desenvolupament prenatal
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
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oai:dugi-doc.udg.edu:10256/146762017-11-30T09:22:18Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Massaguer i Vall-llovera, Anna
Romero Pérez, Inés
Universitat de Girona. Facultat de Ciències
2017-11-30T09:22:18Z
2017-11-30T09:22:18Z
2017-09
http://hdl.handle.net/10256/14676
The prostate cancer (CaP) is between men the sixth cause of cancer death. The diverse treatments used against this neoplasm include mainly toxic effects on the organism, due to acting in both cancer cells and those that are normal. A new focus named “target delivery” tries to solve the problems of conventional treatments. Within therapies, one strategy involves joining antitumour agents to molecules that have an overexpressed receptor in cancer cells, to target them selectively.
In prostate cancer cell line (PC3), the overexpressed receptor “Gastrin releasing peptide” (GRP) has been described, which presents as a ligand a peptide homolog to Bombesin, this one acts as a potent growth factor. The use of peptides derived from the Bombesin would carry antitumor agents specifically to PC3 cells.
It has also been described that several organometallic complexes display antitumour activity depending on photoactivation, which would only allow active compounds on tumor cells when they are irradiated.
The main objective of this study is the characterization of the biological activity of various organometallic compounds based on platinum and ruthenium, conjugated to an analogue peptide to the Bombesin. For this reason, its effect on the viability of prostate cancer cells has been studied, with the use of cell culture techniques. In addition, the ability of the different compounds to be photoactivated has been estimated, by means of its irradiation at λ = 447 nm.
Based on the different techniques used, platinum L compound has been identified as a possible antitumor agent, due to its potent effect on cell viability. In addition, the ability of the ruthenium B complex to be photoactivable has been described, which would classify it as a non-toxic prodrug capable of exerting cytotoxic activity in a controlled way, from its irradiation.
It has also been shown that the conjugation of the different compounds to a peptide analogue to the Bombesin and a sequence of nuclear localization (NLS), increase the internalization and activity of compounds in cancer cells. In addition, both peptides used as carriers can reach the nucleus; being the NLS sequence the cause of a greater accumulation at the nucleus of the compounds transported
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Pròstata -- Càncer -- Tractament
Medicaments antineoplàstics
Bombesina
Compostos organometàl·lics -- Ús terapèutic
Prostate -- Cancer -- Treatment
Antineoplastic agents
Bombesina
Organometallic compounds -- Therapeutic use
Biología
Estudi de l’activitat biològica de compostos organometàl•lics com agents antitumorals vehiculitzats amb anàlegs de la Bombesina
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
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oai:dugi-doc.udg.edu:10256/146772018-04-04T08:11:16Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Massaguer i Vall-llovera, Anna
Salvà i Castro, Judit
Universitat de Girona. Facultat de Ciències
2017-11-30T09:26:08Z
2017-11-30T09:26:08Z
2017-09
http://hdl.handle.net/10256/14677
Nowadays cancer is a global problem being the second leading cause of deaths in the EEUU. Particularly, the
pancreatic cancer (PDAC) causes the 7% of the deaths from cancer. The main problems of the PDAC are its delayed
diagnostic and the low rate of survival in the advanced stages.
It has been noticed that tumoral cells express abnormal glycosylation patterns in their surface. These changes include
an overexpression of some glucidic antigens like Sialyl-Lewis. These antigens are composed by a Sialic acid molecule
linked to the terminal chains of the Lewis sugars (antigens of the Lewis blood group). In fact, there are two types of
Sialyl-Lewis antigens that have been observed to be overexpressed in tumoral cells: SLex and SLea. These antigens are
synthesized by two α-2,3-sialyltransferases enzymes: ST3Gal III and ST3Gal IV.
In this project, we want to analyse the role of the glycosyltransferases (ST3Gal III and ST3Gal IV) in the tumoral
phenotype of pancreatic cancer cells. To achieve this objective, we want to perform a genomic knock-out of these
enzymes using the CRISPR-Cas9 system and compare the phenotype of the control cells with the engineered cells. To
verify the knock-out, we want to check the expression of ST3Gal III and ST3Gal IV at the protein level using a
Western Blot analysis.
To perform genomic editions, it is necessary to transfect BxPC-3 and Capan-1 cells with different vectors that contain
the Cas9 nuclease and the sgRNA. For this reason, different liposomal reagents have been tested using different size
vectors conjugated with GFP. The transfection protocol has been optimized to transfect our cells using a 5.4 kb vector
equivalent to the vector with the sgRNA cloned in it. In contrast, using the 12.1 kb vector, equivalent to the vector
with the Cas9 and the sgRNA cloned, we have achieved low transfection efficiencies. For these reasons, to perform
the edition it has been established to transfect vectors with the sgRNA using the optimized protocol whereas Cas9 has
to be introduced into our cells using another strategy.
In addition, we have optimized the protocol to determine the ST3Gal III expression using the Western Blot technique.
It has been established to use the TBST 5% of BSA blocking solution and low concentrations of secondary antibodies
to get membranes showing the specific band of ST3Gal III
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Pàncres -- Càncer -- Investigació
Glucosiltransferases
Genòmica
Cèl·lules canceroses
Pancreas -- Cancer -- Research
Glycosyltransferases
Genomics
Cancer cells
Biología
Anàlisi del paper de les glicosiltransferases (GT) en el fenotip tumoral de cèl•lules de càncer de pàncrees mitjançant el seu silenciament
info:eu-repo/semantics/bachelorThesis
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oai:dugi-doc.udg.edu:10256/146782018-04-04T08:10:56Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Castro Gallegos, Jessica
Puig i Miquel, Teresa
Ruiz Martínez, Santiago
Trias Alguacil, Maria del Mar
Universitat de Girona. Facultat de Ciències
2017-11-30T09:31:02Z
2017-11-30T09:31:02Z
2017-09
http://hdl.handle.net/10256/14678
Lung cancer is the most common cancer and the leading cause of cancer death worldwide. Lung
adenocarcinoma is the most frequent type of non-small cell lung cancer (NSCLC) and the one with
a higher incidence in non-smokers. The Epidermal Growth Factor Receptor (EGFR) may harbor
activating mutations on tyrosine kinase domain, which trigger loss of growth control through
sustaining activation of EGFR-related signaling pathways. In these cases, epidermal growth factor
receptor tyrosine kinase inhibitors (EGFR-TKI), such as gefitinib are administrated. Thus, EGFRTKI
selectively blocks tumor cell signal transduction. However, a secondary mutation in EGFR can
provide resistance to EGFR-TKI in tumor cells. This situation has led to search for alternatives to
slow down the progression of resistant cells. A promising target to do so is fatty acid synthase
(FASN), an overexpressed enzyme in tumor cells that synthesize fatty acids de novo, instead of
acquiring them from the diet. FASN inhibition allows to reduce the energy contribution that helps to
get the excessive growth of the cell.
In order to overcome resistance to gefitinib, the objective of this project is to analyze in vitro the
effect of gefitinib and G28, a FASN inhibitor, on PC9 cell line and on three PC9-derived cell lines
resistant to gefitinib: PC9-GR1 and PC9-GR4, both carrying T790M secondary mutation, and PC9-
GR3. It has been studied the effect of gefitinib and G28 on the expression levels of EGFR and
FASN, their respective targets, by Western Blot analysis. Also, it has been evaluated the effect of
these drugs on apoptosis induction (PARP cleavage) and on key proteins of the PI3K/AKT and the
Ras/MAPK signaling pathways. To do so, treatments with the IC50 of each drug for 72 hours and
with ten times IC50 for 24 hours have been performed.
Generally, the results obtained show that gefitinib reduces phosphorylation of EGFR and induces
apoptosis in all cell lines. Gefitinib blocks EGFR-derived signaling pathways in all cell lines, in 24-
hour treatment.
The G28 treatment clearly diminishes FASN levels and induces apoptosis in all cell lines. The 24-
hour treatment reduces EGFR activation, that inhibits its related signaling pathways in all PC9
resistant cell lines
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Adenocarcinoma
Pulmons -- Càncer
Factor de creixement epidèrmic
Medicaments antineoplàstics
Apoptosi
Àcids grassos
Lungs -- Cancer
Epidermal growth factor
Antineoplastic agents
Apoptosis
Fatty acids
Biología
Estudi de la inhibició de la sintasa d'àcids grassos (FASN) per revertir la resistència al gefitinib en adenocarcinoma de pulmó
info:eu-repo/semantics/bachelorThesis
NO
LICENSE
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2018-04-04 10:10:56.534
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oai:dugi-doc.udg.edu:10256/146792017-11-30T09:34:45Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bañeras Vives, Lluís
Beemster, Gerrit
Vidal Amill, Anna
Universitat de Girona. Facultat de Ciències
2017-11-30T09:34:45Z
2017-11-30T09:34:45Z
2017-09
http://hdl.handle.net/10256/14679
Zea mays is one of the most important agricultural crop-species for the human diet, but little is known about the endophytes associated with maize. Within the last few years, endophytes are becoming more interesting as they can change our understanding about the relationship between bacteria and plants. There are a lot of studies with regards to this topic from the last couple of years, but not much is known on the role that these microorganisms develop colonising a particular cultivar, or their interaction with the host’s genome. The main objective of this project was to find evidence of bacterial endophyte organisms, and developing a PCR based protocol for isolating bacteria within the leaf growth zone of maize. This area has been chosen for this project since it has not been studied before in terms of endophytes. This project describes non-maize related sequences, extracted from maize leaf material, which could provide evidence for endophytes. In order to find robust evidence, firstly, next generation sequencing (NGS) data were mapped against the maize reference genome [Taxon ID: 4577]. The sequences that did not align against the maize reference genome, were the ones used during the study. These sequences were BLASTed (Boratyn , et al. 2012) using the NCBI database to get a taxonomic report. The most significant alignment was for Desulfitobacterium hafniense. To support this, various PCR protocols were designed by using two different kind of primers: general bacterial primers (A) and specific bacterial primers (B), which avoid chloroplast gene amplification. These were then sequenced with Illumina. The best alignments for general bacterial (A) primers were for 16S rRNA chloroplast genes and uncultured bacteria, which did not give useful information. However, when using the specific bacterial primers (B), the best hits were for uncultured bacteria clone FFCH13347. Since we were not able to get the same result as in the NGS data taxonomic study, we are not able to confirm the presence of Desulfitobacterium hafniense in the leaf growth zone or the presence of other bacteria organisms. Further analysis about possible endophytes within the leaf growth zone are thus needed
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Previous issue date: 2017-09
application/pdf
eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Endòfits
Blat de moro -- Microbiologia
Endophytes
Corn -- Microbiology
Biología
The Search for possible endophytes in the growth zone of maize leaves
info:eu-repo/semantics/bachelorThesis
info:eu-repo/semantics/publishedVersion
NO
LICENSE
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oai:dugi-doc.udg.edu:10256/14679
2017-11-30 10:34:45.178
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oai:dugi-doc.udg.edu:10256/152152018-04-04T08:04:31Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Muñoz Frigola, Marta
Ferrer Morell, Júlia
Universitat de Girona. Facultat de Ciències
2018-03-19T11:41:52Z
2018-03-19T11:41:52Z
2018-02
http://hdl.handle.net/10256/15215
The present study analyses the oocyte volumetric response to the different stages of development in Molva macrophthalma, a cold water species found in the north-western of the Mediterranean Sea.
The same research has also been conducted in a phylogenetically very close species, Molva dypterygia, which is distributed in the northeast of the Atlantic Ocean and part of the Arctic Ocean, in order to compare them. The methodology used is based on the analysis of images and, more specifically, on the "Ovarian Packing Density" or OPD. Which, according to the Delesse Principle, that states that the fractional volume of the oocytes is proportional to its fractional cross-sectional area and the calculation of its diameter, allows to determine the number of oocytes per gram of ovary
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Gadiformes -- Reproducció
Gadiformes -- Reproduction
Biología
Anàlisi volumètrica dels estadis de desenvolupament oocitari en Molva macrophthalma i M. dypterygia
info:eu-repo/semantics/bachelorThesis
NO
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oai:dugi-doc.udg.edu:10256/152162018-04-04T08:02:28Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pons Ferran, Pere
Lucha Ruiz, Esther
Universitat de Girona. Facultat de Ciències
2018-03-19T11:51:20Z
2018-03-19T11:51:20Z
2018-02
http://hdl.handle.net/10256/15216
In the last years, the practice of removing all the deadwood after wildfire and use it with energetic purposes has increased. With the objective to evaluate and decrease the impacts of these practices upon biodiversity, from the University of Girona the project Anifog has been created, conducted by the Pecat research team, where a Handbook of Good Management Practices in burned forests has been done to guide managers and forest workers. In my Final Grade Work has been evaluated how two different management practices following the handbook recommendations affect the diversity of saproxylic beetles. The first management consists on logging dead trees or those damaged by the wildfire leaving part of the wood on-site (Sustainable logging), and the second one consists on leaving the forest unaltered after the fire (Non-intervention). Saproxylic beetles have an important ecological role in the recycling of nutrients found in deadwood and, therefore, are important for the recovering of the ecosystem after a wildfire. Furthermore, they are used in our study as bioindicators of the deadwood and its associated biodiversity
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eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Boscos -- Gestió -- Mediterrània, Regió
Biodiversitat -- Conservació
Escarabats -- Conservació
Indicadors biològics
Forest management -- Mediterranean Region
Biodiversity conservation
Beetles -- Conservation
Indicators (Biology)
Biología
Assessing the response of beetle community to the management of a Mediterranean burned forest
info:eu-repo/semantics/bachelorThesis
east=2.7772215999999617; north=41.7144136; name=Santuari Mare de Déu del Vilar (Blanes)
NO
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oai:dugi-doc.udg.edu:10256/152172018-04-04T08:03:41Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Abril, Sílvia
Mayol Pérez, Júlia
Universitat de Girona. Facultat de Ciències
2018-03-19T11:58:31Z
2018-03-19T11:58:31Z
2018-02
http://hdl.handle.net/10256/15217
The Amazon jungle is a zone of great importance in regards of its diversity; due to its climatic characteristics it offers optimal conditions to a big number of species, animal and vegetal. In particular the chiropters, present an elevated diversity and abundance in the neotropic zones and their populations are fundamental to the good functioning of the communities and even the ecosystem. Especially those that belong to the family Phyllostomidae, the majority frugivorous who have a huge importance in the seed dispersion and, with that, they intervene in the regeneration and succession of the forest. The population of chiropters it’s in decline due to being subjugated to a combination of menaces occasioned mainly by human activity, the majority of said menaces are related to the alteration of nature, and so, directly related with the bat’s habitat perturbation. The main objective of this study is to determine the diversity of the chiropters of two sampled zones in San Rafael, the Community and its communal Reserve. To be able to determine if there are differences between both studied zones and analyze if said differences have been occasioned by the anthropization of the area
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application/pdf
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Biologia (TFG)
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
Ratapinyades -- Poblacions -- Amèrica del Sud
Biodiversitat -- Conservació
Indicadors biològics
Bat populations -- South America
Biodiversity conservation
Indicators (Biology)
Biología
Estudi de la diversitat de quiròpters de sotabosc de la Comunitat amazònica de Sant Rafael i la seva Reserva comunal
info:eu-repo/semantics/bachelorThesis
east=-60.033333; north=-3.166667; name=Selva amazònica
NO
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oai:dugi-doc.udg.edu:10256/162272019-01-24T13:20:41Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Aliu Mancebo, Gerard
Universitat de Girona. Facultat de Ciències
2019-01-24T13:20:41Z
2019-01-24T13:20:41Z
2018-06
http://hdl.handle.net/10256/16227
The increase in biofuel demand in recent decades is one of the main reasons why post-fire management is based on the mass extraction of the affected biomass. This invasive management has a direct influence on the biodiversity of the ecosystem and on the community structures. The epigeous arthropods of the Formicidae family are essential elements of the forest and are usually used as bioindicators because of their high sensibility to environmental changes and to allow the interpretation of the changes caused by a disturbance. This bioindicator capacity is used to evaluate the impact of the fire occurred in summer 2016 in the area of Vilar de Blanes area of summer 2016 and the subsequent forest management. The differences between plots managed with the “Fitxa de Bones Pràctiques” (Good Practice Sheets) of the Anifog project and plots without intervention were evaluated, as well as the differences between three microhabitats: regrowth of Viburnum tinus or Arbutus unedo, empty soil and pile of burnt branches. An analysis of the sampled species abundance in each of the microhabitats was carried out and no significant differences were observed between treatments. In contrast, the diversity indices did define a greater diversity in the microhabitats with management, being the microhabitat of Regrowth the most representative. The distribution patterns of the community species in the different microhabitats can be explained through their ecological action and their diet. Thus, predator or scavenger species turned out to be more explanatory for the Regrowth and Pile habitats, while the omnivorous diet did not show significant preferences. In addition, a comparative analysis between this fall sampling and a spring sampling was performed to see if the management is favorable over time. Although the similarity studies showed clear differences between seasons, this dissimilarity can’t be attributed to the performed management, since the seasonality modifies the activity patterns of the species of the community. Therefore, in order to assess the efficiency of the post-fire management, it is recommended to do annual samplings in order to monitor the evolution of this type of management
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Previous issue date: 2018-06
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Formigues -- Efecte dels incendis
Repoblació forestal -- Catalunya -- Blanes
Ecologia del foc -- Catalunya -- Blanes
Ants -- Effect of fires on
Fire ecology -- Catalonia -- Blanes
Reforestation -- Catalonia -- Blanes
Biología
Avaluació de la gestió forestal postincendi del Vilar de Blanes: anàlisi de la diversitat i la composició de la comunitat de formícids
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.7772128582000732; north=41.71441860972947; name=blanes
NO
LICENSE
license.txt
license.txt
text/plain
1748
https://dugi-doc.udg.edu/bitstream/10256/16227/3/license.txt
8a4605be74aa9ea9d79846c1fba20a33
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CC-LICENSE
license_rdf
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AliuMancebo.Gerard.pdf
AliuMancebo.Gerard.pdf
application/pdf
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oai:dugi-doc.udg.edu:10256/16227
2019-01-24 14:20:41.493
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162332019-01-25T08:34:57Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bañeras Vives, Lluís
Casanovas Jiménez, Adrià
Universitat de Girona. Facultat de Ciències
2019-01-25T08:34:57Z
2019-01-25T08:34:57Z
2018-06
http://hdl.handle.net/10256/16233
Recently, the interest for alternatives to fossil fuels has been increased. One of the alternatives that are investigated is the use of biofuels, which can be produced by electrotrophic microorganisms in a bioelectrochemical system (BES). In BES, bacteria take the reducing power from a cathode to reduce CO2 into organic compounds. One of the most interesting microorganisms in this process is the homoacetogenic Sporomusa ovata, which can produce acetate with a highly efficient production rate. In this work, we aimed at investigating, at the molecular level, what were the most relevant electron carriers to participate in electron transport in inorganic conditions. The general goal of the project were this research was developed was to increase electron capturing by genetic modification and overexpression of relevant candidate genes. We carried out a throughout study of changes in the expression of candidate genes coding for electron-transporter proteins localized at the membrane and the periplasmic space of S. ovata. Specific primers for 27 candidate genes were designed and their specificity was checked through PCR. Changes in the relative expression of those genes (candidate gene/housekeeping gene) were investigated and compared in S. ovata cultures exponentially grown under autotrophic and heterotrophic conditions. Results showed that genes codifying for hydrogenases (e.g. hydA), some ferredoxins (Rnf complex), and c-type cytochromes, were highly transcribed levels in autotrophic conditions. Those genes were postulated as good candidates to capture cathode electrons and would probably be important to be considered in order to optimize S. ovata electron capturing. Due to the poor stabilization of S. ovata cells in the cathode compartment we could not confirm to what extent HydA, Rnf, and Cytochrome c are really participating in electron capturin
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CasanovasJiménez.Adrià.pdf: 1455069 bytes, checksum: 989445cfc36e6cec7296289f2bcf0f8b (MD5)
Previous issue date: 2018-06
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Biotecnologia microbiana
Biocombustibles
Bacteris anaerobis -- Aspectes genètics
Microbial biotechnology
Biocarburants
Anaerobic bacteria -- Genetic aspects
Biología
Anàlisi dels principals gens involucrats en el transport d’electrons sota condicions anàlogues a l’electrosíntesi microbiana en S. Ovata
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
text/plain
1748
https://dugi-doc.udg.edu/bitstream/10256/16233/3/license.txt
8a4605be74aa9ea9d79846c1fba20a33
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CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
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ORIGINAL
CasanovasJiménez.Adrià.pdf
CasanovasJiménez.Adrià.pdf
application/pdf
1455069
https://dugi-doc.udg.edu/bitstream/10256/16233/1/CasanovasJim%C3%A9nez.Adri%C3%A0.pdf
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oai:dugi-doc.udg.edu:10256/16233
2019-01-25 09:34:57.197
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162342019-01-25T08:39:27Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Barrabés Vera, Sílvia
Fàbrega Ribas, Anna
Universitat de Girona. Facultat de Ciències
2019-01-25T08:39:27Z
2019-01-25T08:39:27Z
2018-06
http://hdl.handle.net/10256/16234
The Epidermal Growth Factor Receptor (EGFR) is a glycoprotein that belongs to the family of tyrosine kinase receptors and that it is usually altered in tumor cells. It has been reported that its inhibition decreases tumor proliferation and thus, it is an attractive target for antitumor therapies. There are currently two main strategies of EGFR inhibition: monoclonal antibodies and tyrosine kinase inhibitors (TKI). Unfortunately, the appearance of resistance and other factors limit often the effectiveness of these treatments. In order to study how certain changes in EGFR can affect target therapies against this receptor, we must understand its conformational dynamics. EGFR activation requires the binding of two monomers that, induced by the presence of a ligand, allow then the interaction of the kinase domains and initiate the downstream signaling. However, the deregulation of this receptor may be modulated by several factors, such as the glycosylation of its ectodomine. Tumor progression has been related with an alteration of EGFR glycosylation and it has been observed that aberrant glycosylations may affect both the binding of the ligand and its activation. Accordingly, to study the overall effect of glycosylation on the response to EGFR directed treatments requires a more profound investigation. This is the objective of this Degree Final Project. Based on the existing bibliographic information, it is widely accepted that the site and type of glycosylation influence EGFR conformation and activity. In spite of the controversy in some studies, it seems that terminal fucosylation and sialylation decrease the activity of this receptor. In contrast, other glycosylations such as core fucosylation and Lewis epitopes, increase the activity of EGFR. Moreover, it seems that the alteration of glycosylation could have an effect on EGFR response to treatments. According to current information, EGFR glycosylation does not seem to alter the binding of antibodies to the extracellular domain. However, studies on another member of the EGFR family, HER2, show the opposite. The most relevant result obtained in this project is that extracellular glycosylation of EGFR indirectly affects intracellular sensitivity to TKI. Finally, the need to find alternative treatments to fight emerging resistance and the demand for higher treatment specificity opens an avenue for glycosylation itself as a potential novel target against cancer
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Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Glicosilació
Factor de creixement epidèrmic -- Receptors
Càncer -- Tractament
Epidermal growth factor -- Receptors
Glycosylation
Cancer -- Treatment
Biología
Efecte de la glicosilació sobre l’activitat i la resposta a tractaments de l’EGFR
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
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license.txt
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oai:dugi-doc.udg.edu:10256/162352019-01-25T08:43:19Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Ribó i Panosa, Marc
Montesinos Costa, Mercè
Feliu Manubens, Judit
Universitat de Girona. Facultat de Ciències
2019-01-25T08:43:19Z
2019-01-25T08:43:19Z
2018-06
http://hdl.handle.net/10256/16235
Chronic kidney disease (CKD) is currently a public health problem worldwide. CKD is a silence disease that does not cause any pain, which is why it is very difficult to be diagnosed by the patient thus early detection of chronic kidney disease is an important key to avoid further progression.
CKD is defined as a progressive, permanent and irreversible loss of the glomerular filtration rate over a variable time, sometimes even years, expressed by a reduced clearance of creatinine < 60 ml/min/1,73m2.
Glomerular filtration is the best index of measurement of renal function because it provides a measure of the kidney’s filtering capacity and perfectly reflects the functioning renal mass. However, the measurement of glomerular filtration is difficult to carry out and so alternatives have been developed. Among the methods that allow us to estimate glomerular filtration there are serum creatinine and estimation using equations such as CKD-EPI.
In this work parameters such as "serum creatinine" and "estimation of the glomerular filtration CKD - EPI" have been studied as a measure of chronic kidney disease for the population of different parts of Catalonia between the ages of 15 to 104 years. It has been found that the relationship between these two parameters is illustrated in a hyperbola curve. This implies the need for abrupt reduction in glomerular filtration for an increase of creatinine above universally established reference values.
On the other hand, serum creatinine is a parameter with a high individual influence and this is why it should be analysed from the change reference value. This new method would allow detecting cases of chronic renal insufficiency in patients where serum creatinine values are still within the universal reference ranges.
Finally, the distribution of CKD in the population studied according to age and sex has been studied. It has been shown that age is a susceptibility factor in the progression of CKD, that both sexes have a very similar distribution for the affectation of this disease and that the majority of the population is in the third stage of the classification of the CKD
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Creatinina
Insuficiència renal crònica
Creatinine
Chronic renal failure
Biología
Valoració del filtrat glomerulal estimat (FGe) i la creatinina sèrica en la insuficiència renal crònica
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
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oai:dugi-doc.udg.edu:10256/162362019-01-25T09:02:31Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Abril, Sílvia
Crailsheim, Dietmar
Fernández Fernández, Pablo
Universitat de Girona. Facultat de Ciències
2019-01-25T08:50:56Z
2019-01-25T08:50:56Z
2018-06
http://hdl.handle.net/10256/16236
The common chimpanzee (Pan troglodytes) is a species considered “endangered” by the International Union for the Conservation of Nature (IUCN). Its populations have decreased in the last years because of the poaching, illness and habitat loss. This is the reason why ex situ protection measures are being carried out, creating communities where the primates can develop the typical behaviour of the species and satisfy their social nature. But forming these groups is a complex process influenced by many factors, therefore it could have critical implications for the individuals if done in a negligent way.
During the month of July 2017 was concluded at the Primate Recuperation Centre of Mona Foundation the introduction of two chimpanzee females within a group of five males. A study of this community members was performed with the objective of evaluating the result of this integration process and to determine the group welfare. To make this, the registers obtained during the observations made at the centre, during the months of March and April of 2018, were compared with the ones of the 2016-2017 period, previous to the female transfers, and the ones of the months of July, August and September of 2017, first months after concluding the introduction.
The experimental part of this study was carried out by multifocal scan sampling with instantaneous register of the behaviour of the individuals. It has been evaluated the evolution of the group behaviour and the welfare by using of generalized linear mixed models (GMLL). This analysis showed that the individuals had changed their individual and social behaviour and the group welfare because of the process, but without the influence of the subject origin in this change. Furthermore, a Fisher exact test evidenced the dependence between the role of the chimpanzee in the integration and the change in the individual behaviours.
The study has confirmed the good management performed by the workers of the centre, showing that the chimpanzees have adapted to the new group maintaining the previous welfare at the community. However, the social integration of the individuals is not completed yet, therefore more time will be needed to consolidate and stabilize the group
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application/pdf
spa
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Ximpanzés -- Hàbits i conducta
Animals salvatges en captivitat
Espècies amenaçades
Chimpanzees -- Behavior
Captive wild animals
Endangered species
Biología
Estudio del comportamiento en un grupo de chimpancés (Pan troglodytes) cautivos tras la introducción de dos hembras: integración y benestar
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.8172061000000213; north=41.9016224; name=Fundació Mona
NO
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oai:dugi-doc.udg.edu:10256/162382019-01-25T08:58:37Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Garcia-Gil, L. J.
Alós i Hernández, Llúcia
Gómez Esteve, Sara
Universitat de Girona. Facultat de Ciències
2019-01-25T08:58:37Z
2019-01-25T08:58:37Z
2018-06
http://hdl.handle.net/10256/16238
This project is focused on studying the importance of FISH technique (Fluorescence In Situ Hybridization) for the diagnosis of cutaneous melanomas and the determination of ambiguous cases. The study begins with the complete protocol that is followed in the resolution of a complex case, since the sample arrives at the Pathological Anatomy Department of Hospital Clínic of Barcelona until the final report for the patient is concluded. This protocol deals with an initial microscopic study, an immunohistochemical analysis and, in case of a non-conclusive diagnosis, a FISH technique is implemented. Thus, the diagnostic criteria observable under the microscope that helps to differentiate malignant tumors from benign ones are exposed, emphasizing that it is not always possible to conclude this difference without the use of FISH. To analyze it, a study was carried out with 10 cases, of which 3 of them have been melanoma and the remainder have been benign. In almost all of them (with one exception) it has been essential to use the technique studied to solve them. Of these cases, there are some that have allowed us to demonstrate that histological and immunological studies are often inadequate to recognize the seriousness of the tumor and that, in these cases, the use of a technique such as FISH can be decisive for a correct diagnosis. Simultaneously to these cases, one geographic study has been carried out. It refers to the number of hospitals throughout Spain that use FISH to diagnose cutaneous melanomas. A bibliometric study has also been carried out and it includes a set of outstanding articles that focus on emphasizing the importance of this technique in this type of diagnosis. From these studies the year of publication has been valued with the intention of knowing the interest that FISH has aroused around the world and since when it began to be studied in depth. With this project it is possible to confirm the hypothesis of the relevance of the technique in this field and makes remarkable the lack of its use and study, in contrast to the positive results obtained
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Previous issue date: 2018-06
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Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Melanoma -- Diagnòstic
Hibridació in situ per fluorescència
Melanoma -- Diagnosis
Fluorescence in situ hybridization
Biología
Utilització de sondes fish per al diagnòstic de melanoma cutani a partir de casos reals
info:eu-repo/semantics/bachelorThesis
Estudis de grau
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NO
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2019-01-25 09:58:37.229
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162392019-01-25T09:23:21Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Heras Mena, Sandra
Alsius Suñer, Mercè
Jiménez Castro, Marc
Universitat de Girona. Facultat de Ciències
2019-01-25T09:23:21Z
2019-01-25T09:23:21Z
2018-06
http://hdl.handle.net/10256/16239
Congenital defects can be caused by chromosomal anomalies, which are classified in numerical or structural abnormalities, depending on whether they affect, if may affect the number of chromosomes or their structure. For the correct detection of these defects before birth prenatal diagnosis is used, which allow detection in the fetus.
Prenatal screening is technique that shows the pregnant women if there is a risk of suffering an aneuploidy over the total number of pregnant women in the population. Subsequently, this risk can be confirmed by a diagnostic test in cases where the risk increases.
The main objective of this study is the preparation of the evaluation report of the screening program for the year 2017 at the “Laboratori Clínic Territorial de Girona” based on the calculated screening and their origin, the monitoring of positive screenings, the results of the pathological karyotypes and an analysis of the quality indicators.
In addition, an analysis of the reasons for the not calculated screening due to lack of the ecography searching for possible improvement actions through the writing of a report to Obstetrics services. And finally, an analysis of how can affect the activity the implementation of the aneuploidies prenatal screening by fetal DNA in maternal blood according to the diagnostic algorithm approved by CatSalut in 2017, and the Head of Service of the “Laboratori Clínic Territorial de Girona”.
More over with the analysis results, the main origins and the number of abnormal karyotypes that occurred in 2017 have been identified. The sensitivity and specificity of the aneuploidy screening program in this laboratory has also been evaluated. In addition, it was possible to identify the main reasons for the 265 screenings that were not calculated by pending ultrasound and we studied possible actions to improve the results obtained.
Finally, it has been verified that the implementation of the aneuploidies screening by fetal DNA in maternal blood would mean the increase in activity and also the reduction of the number of false negatives and therefore a greater detection of a trisomy
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Previous issue date: 2018-06
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Aneuploïdia
Cromosomes humans -- Anomalies
Diagnòstic prenatal
Aneuploidy
Human chromosome abnormalities
Biología
Avaluació del programa de cribratge d’aneuploïdies bioquímicecogràfic de l’any 2017 i plantejament de possibles accions de millora
info:eu-repo/semantics/bachelorThesis
Estudis de grau
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NO
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2019-01-25 10:23:21.964
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162412019-01-25T09:30:03Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Gómez López, Crisanto
Montori i Faura, Albert
Jordà Remírez, Adrià
Universitat de Girona. Facultat de Ciències
2019-01-25T09:30:02Z
2019-01-25T09:30:02Z
2018-06
http://hdl.handle.net/10256/16241
On a worldwide level, significant biodiversity loss is occurring (Wake, 1991). In this biodiversity crisis, amphibians are the most disadvantaged group, with 41% of the species in this group considered to be under threat (IUCN, 2017).
In this sense, the delta plain of the Llobregat is not an exception and regarding the autochthonous species present in the early 20th century (Alytes obstetricans, Bufo spinosus, Epidalea calamita, Hyla meridionalis, Pelobates cultripes and Pelophylax perezi), three of them (Bufo spinosus, Epidalea calamita and Pelobates cultripes) have disappeared from the delta plain, two of them (Alytes obstetricans and Hyla meridionalis) have been reduced to a residual population and only one of them (Pelophylax perezi) is found in good condition. Moreover, in 2005, the presence of exotic species, Discoglosus pictus (Franch et al., 2007a), was detected.
This loss of amphibian richness can be explained by the fragmentation and loss of terrestrial habitats (Sinsch, 1992; Baker and Halliday, 1999) caused by the changes in land use and loss of ecological connectivity, due to loss of quality and pollution of aquatic systems that hinder the development of amphibian populations (Sadinski and Dunson, 1992; Skelly, 1996; Joly et al., 2001; Oldham, 1999) and for the increase in the presence of invasive species (Montori et al., 2009).
In the Llobregat Delta, the fragmentation and loss of habitats are explained by a significant increase in population and development over the course of the 20th century, which has led to changes in the needs of the population that have caused an increase in the surface area destined for industrial zones, for services and for the port and airport, to the detriment of natural and agricultural areas. This variation in land use, together with erroneous administrative decisions in the management of natural areas, has caused a significant loss of ecological connectivity among amphibian populations. On the other hand, the loss of quality and degradation of aquatic systems that hinder the presence of amphibians on the delta plain is clearly reflected in the salinity, the high ammonia concentrations and the wide pH range observed at different water points in the delta. Moreover, the presence of several exotic species such as Procambarus clarkii, Trachemys scripta, Gambusia holbrocki and Discoglossus pictus among others has also contributed to the decline of amphibians to the Llobregat delta for predation and competition
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Amfibis -- Poblacions -- Catalunya -- Llobregat, Delta del
Amfibis – Hàbitat -- Catalunya -- Llobregat, Delta del
Animals invasors -- Catalunya -- Llobregat, Delta del
Amphibian populations -- Llobregat River Delta (Spain)
Amphibians -- Habitat -- Llobregat River Delta (Spain)
Introduced organisms -- Llobregat River Delta (Spain)
Biología
Pèrdua de biodiversitat d’amfibis al Delta del Llobregat
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.1194869999999355; north=41.3064397; name=El delta del Llobregat
NO
LICENSE
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license.txt
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oai:dugi-doc.udg.edu:10256/162422019-01-25T09:35:53Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pons Ferran, Pere
Miralles Alvarez, Anna Maria
Universitat de Girona. Facultat de Ciències
2019-01-25T09:35:53Z
2019-01-25T09:35:53Z
2018-06
http://hdl.handle.net/10256/16242
After a fire, in southern Europe, the most common way to manage a burned forest is a salvage logging with heavy machinery and whole-tree harvesting, which eliminates almost all the biomass present in the ecosystem. There are also other methods less used such as nonintervention, which leaves the forest in the conditions in which it has remained after the disturbance. Another post-fire management provided by the Anifog project, of the University of Girona, is the treatment of good practices, using light machinery and stem-only harvesting, leaving woody debris in the form of piles in the area.
To compare the efficacy of the three treatments in reference to the regeneration of the forest, an area near Blanes (Girona, Catalonia), burned in July 2016, was chosen, and the differences in the removal of acorns by rodents were studied because these seeds (if they were in the right conditions) could then germinate and thus help return the forest to its initial state.
Ten plots were established and distributed to manage them differently for each treatment. Each forest management had 20 stations under shrub resprouts, and the treatment of good practices had also 20 stations under branch piles. The collection of 3 variables was carried out: rate of removal of acorns, distance from the acorn to the station and micro-habitat where the acorn was found, in two experiments (autumn 2017 and spring 2018). After treating the data statistically, it was found that in the last two groups of data mentioned, the methodology was not successful and, therefore, the results could not be representative due to lack of samples. On the other hand, regarding the rate of removal, the results were of more acorn removal in the case of the stations under the branch piles of the treatment of good practices, and significantly less in the conventional treatment.
All in all, it could be said that the treatment of good practices and, specifically, the presence of branch piles is favourable for the removal of acorns by rodents and could have positive effects for a faster natural regeneration of the forest if the dispersal compensates predation. Aversely, conventional treatment seems to have a negative impact on the dispersion of seeds, they are removed more slowly; therefore, it could mean that it is not the best management option for rodents and their ecosystem services
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Incendis forestals -- Catalunya -- Blanes
Rosegadors -- Efecte dels incendis
Llavors -- Disseminació
Repoblació forestal -- Catalunya -- Blanes
Ecologia del foc -- Catalunya -- Blanes
Forest fires -- Catalonia -- Blanes
Rodents -- Effect of fires on
Seeds -- Dispersal
Reforestation -- Catalonia -- Blanes
Fire ecology -- Catalonia -- Blanes
Biología
On s’emporten les llavors els rosegadors quan es talla el bosc cremat?
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.7772128582000732; north=41.71441860972947; name=Santuari Mare De Déu Del Vilar
NO
LICENSE
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oai:dugi-doc.udg.edu:10256/162432019-01-25T09:40:05Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Colomer, Jordi
Müller, Mara Franziska
Universitat de Girona. Facultat de Ciències
2019-01-25T09:40:05Z
2019-01-25T09:40:05Z
2018-06
http://hdl.handle.net/10256/16243
Daphnia magna is a key zooplankton organism found in many freshwater aquatic systems. Its feeding usually includes phytoplankton as well as bacteria, but Daphnia are also able to feed on wastewater particles, which is why they have recently been considered as potential organisms for tertiary biologically based wastewater treatment. Even though D. magna is a well-studied species, little is known about the possible effect of the flow environment on its fitness. This study focuses on analysing the effect of zero mean turbulence on the filtration capacity, the swimming velocity and the 24h-survival of D. magna individuals with three different body lengths (L = 1.2, 1.5 and 1.8 mm). Results show a clear difference between the response to low or high turbulent shear rates. At low shear rates, D. magna individuals were able to swim more or less freely, especially the bigger ones, and the filtration rate increased, due to the greater Daphnia-particle encountering frequency. Hereafter, at low shears the swimming velocity of D. magna depended positively on the shear rate and negatively on the body length. On the other hand, at high shear rates D. magna individuals were not able to overcome the flow, thus presenting similar swimming speeds independent of the body length, and the filtration capacity was completely inhibited. The survival of the D. magna individuals followed the same trend, that is, at low shear rates individuals presented lower mortality than at high shear rates. However, smaller individuals showed to be more sensitive to the flow environment, as they presented higher mortality at low shear rates than the bigger individuals. In contrast, bigger D. magna individuals presented higher mortality at high shear rates, proving to be more vulnerable to high shears
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application/pdf
eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Aigües residuals -- Depuració -- Tractament biològic
Zooplàncton d'aigua dolça
Daphnia magna
Sewage -- Purification -- Biological treatment
Freshwater zooplancton
Biología
Daphnia Magna responses to zero-mean flow turbulence
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
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oai:dugi-doc.udg.edu:10256/162442019-01-25T09:42:22Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pinart Nadal, Elisabeth
Portavella Gramunt, Alejandra
Universitat de Girona. Facultat de Ciències
2019-01-25T09:42:22Z
2019-01-25T09:42:22Z
2018-06
http://hdl.handle.net/10256/16244
Testicular spermatozoa of all mammalian species are unable to move progressively or fertilize oocytes. To acquire these two abilities, they must undergo two extratesticular maturational processes: epididymal maturation in the male reproductive tract and sperm capacitation in the female reproductive tract. During epididiymal maturation, spermatozoa undergo structural and biochemical modifications which are modulated by the composition of the epididymal fluid. Among these modifications to highlight: changes in membrane phospholipid composition and in cholesterol/phospholipid ratio, increase in net surface negative charge, increases in the number of disulfide bonds, modification, elimination or addition of surface protein, relocalization of surface antigens, and migration of the cytoplasmic droplet. After this process, spermatozoa are stored in the epididymal cauda until ejaculation.
Epididymal maturation and ejaculation are key events for further capacitation, since only ejaculated spermatozoa are capable to undergo this process. Capacitation is a complex process of structural and functional changes that takes place during the transit through the female reproductive tract, and it is completed when spermatozoa are able to penetrate zona pellucida by undergoing the acrosome reaction. This process involves modifications in sperm surface protein distribution, alterations in the plasma membrane characteristics, changes in enzymatic activity and modulation of expression of intracellular constituents. All these changes occur as a result of the activation of signaling pathways cascades.
Both, epididymal maturation and sperm capacitation are absolutely needed in order to generate fertile spermatozoa. This makes the study of the molecular mechanisms underpinning this two processes very important. This review aims to compile all the information that has been discovered in recent decades about the molecular mechanisms of epididymal maturation and sperm capacitation, and to understand the molecular mechanisms by which spermatozoa acquire fertilizing ability and motility
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application/pdf
eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Espermatogènesi en els animals
Spermatogenesis in animals
Biología
Bibliographic review on the molecular mechanisms involved in epididymal maturation and sperm capacitation: Revisió bibliogràfica sobre els mecanismes moleculars involucrats en la maduració epididimària i la capacitació espermàtica
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
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Portavella Gramunt, Alejandra.pdf
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oai:dugi-doc.udg.edu:10256/162452019-01-25T09:47:22Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Romaní i Cornet, Anna M.
Satorres Salvadó, Marta
Universitat de Girona. Facultat de Ciències
2019-01-25T09:45:14Z
2019-01-25T09:45:14Z
2018-06
http://hdl.handle.net/10256/16245
Decomposition is a very important process for life, is the step from organic matter to inorganic components thanks to the help of microbiota and other factors. Climate change is varying the levels of ultraviolet radiation and the global climate so it is important to study its effects on the basic processes of nature.
The aim of this study was to determine the effect of the radiation and water availability in the microbial decomposition of litter of Phillyrea angustifolia and Arbutus unedo and the effect on colonization just after the abscission by bacteria and the fungus in plants growing under different treatments. Also has focused on analysing the differences between the two species taking into account that they are Mediterranean species and have different leaf structures and climate adaptations.
To analyse it, samples have been prepared under the UV0, UVA and UVAB radiation treatments and the “dry” and “water” water regimes. The carbon of fungus and bacteria of all the samples has been calculated over the course of 4 months and the percentage of organic matter remaining in the samples has been calculated.
The results obtained indicate that the growth in different water treatments and availability of water entails changes in the microbial biomass just after abscission in the leaves of the two species: Phillyrea angustifolia and Arbutus unedo. In Phillyrea, bacteria prefer to colonize leaves under drought treatment while in Arbutus fungi prefer to colonize leaves under humid conditions. Also, exposure to UV-B radiation reduces fungal biomass and tends to reduce organic matter remaining in the decomposition process, especially in drought conditions, while UV-A seems to improve microbial colonization and the loss of weight They also indicate that low water availability reduces microbial colonization during the decomposition process.
The results also indicate significant differences in microbial colonization between the two species. Phillyrea shows a greater weight loss and microbial colonization than Arbutus. This could be caused by the differences in its leaf structure.
The results obtained in this work are part of the first phase of a study, which includes monitoring of decomposition during 12-18 months. In later studies it will be interesting to investigate the effect of the radiation and water availability in the levels of lignin and cellulose in these two species and the amount of bacterial and fungal enzymes in the leaves in order to see more clearly how microbiota interact with both types of leaf structure
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Previous issue date: 2018-06
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Fullaraca -- Biodegradació -- Catalunya -- Gavarres
Ecologia microbiana -- Catalunya -- Gavarres
Plant litter -- Biodegradation -- Catalonia -- Gavarres
Microbial ecology -- Catalonia -- Gavarres
Biología
Efecte de la radiació i la disponibilitat hídrica en la descomposició microbiana de fullaraca de Phillyrea angustifolia i Arbutus unedo
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.9839293999999654; north=41.8870886; name=Massís de les Gavarres
NO
LICENSE
license.txt
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2019-01-25 10:47:22.344
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162652019-01-29T15:28:00Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Pons Ferran, Pere
Fuentes López-Dóriga, Anna M.
Universitat de Girona. Facultat de Ciències
2019-01-29T15:28:00Z
2019-01-29T15:28:00Z
2018-09
http://hdl.handle.net/10256/16265
The European Shag (Phalacrocorax aristotelis) is a seabird species used as an indicator of changes in marine ecosystems. Two subspecies exist in the Iberian Peninsula: P. a. aristotelis, distributed along the Cantabric-Atlantic coast, and P. a. desmarestii, distributed throughout several Mediterranean littoral areas, where is endemic from, being the former one,
the object of this study. This subspecies has a low reproductive population in the rocky cliffs of the Mediterranean coast and is listed as Vulnerable
in the Red Book of the Birds of Spain. Up to now, multiple studies have focused on studying the diet of the nominal subspecies, however, there is not as much information available regarding the diet of the Mediterranean subspecies. Therefore, the purpose of this study is to analyze the diet of th
is subspecies in order to improve the knowledge about its ecology to apply the most appropriate measures for its conservation. Specifically, the main objective of this project is to study the diet of a colony of P. a. desmarestii
located in the Maresme during the non-breeding season. In this way, it is expect to determine if the subspecies of the
Mediterranean has an opportunistic diet, like other studies had been postulated for the Cantabrian-Atlantic subspecies, or it is specialist. In order to observe the composition of the diet, a total of 91 pellets have been analyzed, identifying 9,222 sagittae otoliths of the fish. The results have been compared between three periods: July, September and November. Also, the preys length of the most abundant taxa has been compared between the same three periods. These taxa were: the species Ariosoma balearicum , which belongs to the Congridae family, which represents
the 77.8% of the diet, the Sparidae family the 97%, the Gymnammodytes
genus, which belongs to the Ammodytidaefamily, the 5% and the Trachinidae family the 4.5%.
Finally, it has been concluded that the Mediterranean shag, as the Cantabrian -Atlantic subspecies, has an opportunistic diet based on the
availability of prey species. Additionally, the composition and sizes of these prey species varying according to seasonality, maybe as a result of natural fluctuations in fish populations
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Phalacrocorax aristotelis desmarestii
Ocells marins -- Alimentació -- Catalunya -- Maresme
Egagròpiles -- Identificació
Sea birds -- Feeding and feeds
Egagropills -- Identification
Biología
La Dieta del corb marí emplomallat del Mediterrani (Phalacrocorax aristotelis desmarestii)
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.655317239170472; north=41.61019859830707; name=Calella de Mar
NO
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oai:dugi-doc.udg.edu:10256/162662019-01-29T15:37:51Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Vilar Sais, Lluís
Garcia Jacas, Núria
Vilatersana Lluch, Roser
Galceran Sànchez, Marta
Universitat de Girona. Facultat de Ciències
2019-01-29T15:35:28Z
2019-01-29T15:35:28Z
2018-09
http://hdl.handle.net/10256/16266
The Rhaponticum group includes, among others, the old genus Leuzea and for a long time it has been difficult to define taxonomic relationships between the species. The taxonomy of Rhaponticum coniferum, R. berardioides and R. font-queri is unclear. In addition, in 2011 it was collected a population in the Middle Atlas (Morocco) with some morphological characteristics that differentiate it from R. coniferum.
In this study we implemented the use of integrative taxonomy using morphological and molecular methods to clarify the relationships of the old genus Leuzea. Twenty-eight morphological characters in 112 individual herbarium vouchers have been examined. For the molecular study the nuclear region ETS (External Transcribed Spacer) was chosen and several individuals of each taxa were cloned.
The three species previously described are morphologically different. Genetically, R. berardioides clearly separates of others. In terms of the problem population, it has its own morphological characters, but is genetically very close to R. coniferum.
In our taxonomic proposal we suggest to keep R. coniferum, R. berardioides and R. font-queri as three different species, and to subordinate the problem population as a subspecies of R. coniferum
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Compostes -- Classificació
Leuzea
Compositae -- Classification
Rhaponticum Rhaponticum
Biología
Aplicació de la taxonomia integrativa per la delimitació de noves espècies. Un exemple dins el grup Rhaponticum
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=18.048010500000032; north=34.5531284; name=Mar Mediterrània
NO
LICENSE
license.txt
license.txt
text/plain
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oai:dugi-doc.udg.edu:10256/162672019-01-29T15:41:27Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Bas Lay, Josep Maria
Garriga Pérez, Laura
Universitat de Girona. Facultat de Ciències
2019-01-29T15:41:27Z
2019-01-29T15:41:27Z
2018-09
http://hdl.handle.net/10256/16267
The report below is about the importance of post-fire management of a forest. Specifically, we want to study the impact of the type of post-fire management on mammals. The kind of logging after fire can determine the future development and natural restoration of the forest area, with a concrete impact on the structure of the ecosystem and its niches. It's evaluated in what way three kinds of management, after fire effected in Blanes (summer 2016), can effect on mammals communities: one of the treatments has been well managed (Good Practices), the other has not undergone post-fire management, so dead trees and shrubs (Control) have not been cut, and a last treatment which has been applied aggressive tree cutting (Conventional). Also, following the guide of good practices of the ANIFOG project, it’s evaluated how these mammals take advantage of the woody assemblages and trunks stacked on the ground once the dead wood is cut.
The differences between these three treatments are analysed regarding the activity of mammals, based on the analysis of abundance, wealth and patterns of activity. It's interesting to include the third case of conventional treatment where such type of logging has been done in order to look at the differences as well, since this last case is what usually occurs when a forest has burned. If the treatment of good practices (BP) really favours mammals, it's expected to find a favourable effect in the treatments in which good management has been done, therefore a greater activity of the mammals around the piles of branches and dead trunks will be observed.
After 5 months of sampling, collecting data using trail-camera study and transects combined methodology results that were not expected were observed. It’s analysed statistically these results, too, with ANOVA. It should be noted that it's possible that the results have been altered by environmental and anthropic factors, such as the presence of machinery and humans during the period of management of the areas or the action of temperature and wind, which has caused that the cameras did not work during the 15 days of sampling, causing a loss of days to record animal activity.
This report shows how we can study mammals activity, according to treatment post-fire applied, by not invasive methodology
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Mamífers -- Efecte dels incendis
Repoblació forestal -- Catalunya -- Blanes
Ecologia del foc -- Catalunya -- Blanes
Mammals -- Effect of fires on
Reforestation -- Catalonia -- Blanes
Fire ecology -- Catalonia -- Blanes
Biología
Efecte de la gestió forestal post incendi en els mamífers a l’incendi del Vilar de Blanes
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.7775301000000354; north=41.71465999999999; name=El Vilar (Blanes)
NO
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2019-01-29 16:41:27.636
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oai:dugi-doc.udg.edu:10256/162682019-01-29T15:46:43Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Quintana Pou, Xavier
Puig, Xavier
Gómez Aguilera, Noelia
Universitat de Girona. Facultat de Ciències
2019-01-29T15:46:43Z
2019-01-29T15:46:43Z
2018-09
http://hdl.handle.net/10256/16268
Due to the growing interest in the ecology of mosquitoes and their population dynamics, we conducted a study on the culicid species present in the Baix Empordà rice fields, assessing the temporal variations of their adult populations. We also evaluate the possible influence of different environmental variables and soil cover in a radius of 100, 300 and 500 meters from each sampling point, to include the flight distance of the detected species. Since the importance of meteorological factors such as temperature, wind intensity and precipitation has been described in previous studies, we incorporate data retrieved on the Estartit’s meteorological station. The sample gathering was done by means of 6 Biogents Mosquitaire traps (suction traps) and 15 stations distributed throughout the study area.
A total of 3988 mosquitoes are determined, belonging to seven different species. 99.7% of the trapped individuals are included in the Culex genus. Within this genus, contrary to what might be expected at first, C. modestus dominates over C. pipiens, and C. theileri presents very low population densities. No individuals of the genus Aedes are detected, and Culiseta and Anopheles appear anecdotally.
Among the meteorological variables considered in this study, we find significant results in the cases of minimum temperature, wind intensity and relative humidity, always showing negative correlations with the number of catches. It is important to note that the used variables were collected in a meteorological station located 9 km away from the study area, and therefore the results may stray from the reality in a certain measure. In agreement with the expected results, spatial heterogeneity manifests a negative relation with the number of detected adults, and the forested areas favor the mosquitoes’ presence
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Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Mosquits -- Catalunya -- Aiguamolls de l'Empordà
Arrós -- Conreu
Mosquitoes -- Catalonia -- Aiguamolls de l'Empordà
Rice -- Breeding
Biología
Estudi de la dinàmica poblacional de les poblacions de culícids als arrossars del Baix Empordà
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=3.1689504013108944; north=42.000492941058155; name=Baix Empordà
NO
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2019-01-29 16:46:43.842
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oai:dugi-doc.udg.edu:10256/162692019-01-29T15:50:36Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Viñas de Puig, Jordi
Martínez Guillem, Àlex
Universitat de Girona. Facultat de Ciències
2019-01-29T15:50:36Z
2019-01-29T15:50:36Z
2018-09
http://hdl.handle.net/10256/16269
The fish known as sand eel includes two species known as Gymnammodytes cicerelus and Gymnammodytes semisquamatus. Both are present in the Mediterranean Sea although it’s practically impossible to differentiate them morphologically due to their high similarity, leading to an indiscriminate fishing of both. This is the reason why it’s necessary to be able to differentiate them at molecular level with the aim of determine how many populations of each specie exist in order to avoid the loss of viability of any of them.
The Genetics Ichthyology Laboratory of the University of Girona (LIG-UdG) has designed a mitochondrial marker for this purpose, which has allowed to differentiate the two species and determine that there is only one population for each of them. Following this objective, this paper proposes confirming the molecular differentiation of the two species using nuclear markers.
To perform this, an exhaustive bibliographic search has been carried out in order to find nuclear markers that have been used in previous studies to establish the phylogeny of species genetically related to those of this study. Subsequently, the search of primers of the selected markers has been proceeded and the corresponding DNA extraction of individuals from both species, previously differentiated mitochondrially, has been performed. The optimal PCR conditions have been designed and the amplicons replication delimited by the pairs of selected primers has been performed, in addition, the quality of the PCR products has been verified by agarose gel electrophoresis. Next, the cleaning and purification of the resulting amplicons has been carried out with an ExoSAP protocol and has been followed by a BigDye sequencing protocol to sequence them for subsequent bioinformatic analyses.
With the results obtained it can be concluded, through the construction of phylogenetic trees using the neighbour joining method, that Gymnammodytes cicerelus and Gymnammodytes semisquamatus are two different species within the same genus. At the same time, the distance between these two species has been calculated, along with an outgroup conformed by genetically close species, which has allowed to corroborate the final conclusion of this study
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Sonsos -- Filogènia
Gymnammodytes cicerelus
Gymnammodytes semisquamatus
Sand eel -- Phylogeny
Biología
Cerca i optimització de marcadors genètics per a la diferenciació de les dues espècies de sonso
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=18.048010500000032; north=34.5531284; name=Mar Mediterrània
NO
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2019-01-29 16:50:36.913
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oai:dugi-doc.udg.edu:10256/162702019-01-29T15:54:49Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Peracaula Miró, Rosa
Mattioli Borrull, Romana
Universitat de Girona. Facultat de Ciències
2019-01-29T15:54:49Z
2019-01-29T15:54:49Z
2018-09
http://hdl.handle.net/10256/16270
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Mattioli.Romana.pdf: 1713804 bytes, checksum: 3f0136b6cc3986472ae013d2ada1fdf8 (MD5)
Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Glicosilació
Glicoproteïnes
Pàncrees -- Càncer -- Investigació
Marcadors tumorals
Glycosylation
Glycoproteins
Pancreas -- Cancer -- Research
Tumor markers
Biología
Biomarcadors moleculars no invasius pel càncer de pàncrees
info:eu-repo/semantics/bachelorThesis
Estudis de grau
Pancreatic cancer is one of the most lethal types of cancer, especially in developed countries, and statistics predict that the mortality rate will continue increasing until it becomes the second deadliest cancer. The lack of routine controls, the difficulty in diagnosis, the ambiguity of image techniques, the lack of efficient clinical treatments, and, -in general-, the lack of knowledge about this cancer biology are the main reasons that lead to such low survival rate. The only serum tumour biomarker currently used is carbohydrate antigen 19-9 (CA19-9), which is not sensitive or specific enough to be used as a method of diagnosis, but only as a tool for monitoring the disease. In recent decades, many studies have searched for sensitive and specific tumour biomarkers detectable in fluids, which would allow early diagnosis of the disease. Specifically, many scientists have chosen to study aberrant glycosylation patterns (alterations in the composition or structure of glycans or their density in a protein) from glycoproteomic studies, since they have been seen to play an important role in the development of cancer, promoting the invasiveness, chemoresistance and modulating the immune response. The objective of this paper is to discuss the most promising future perspectives in the field of biomarkers, more specifically, addressing it to the altered glycosylation patterns, for the detection of pancreatic cancer.
Many candidates have been proposed to be optimal markers, which include glycoproteins such as RNase1, various acute phase proteins such as α-1 acid glycoprotein, haptoglobin or fetuin, among others. More recent studies have focused on mucins, which are carriers of carbohydrate antigens that are overexpressed in the presence of a tumour, and on other serum glycoproteins such as leukemia inhibitory factor receptor (LIFR), centrosomeassociated protein (CE350) and vacuolar protein sorting-associated protein 13A (VP13A), which show an altered glycosylation pattern in pancreatic cancer patients. The possibility of combining several markers, specifically CA19-9 with CEA, CA125 or with IGF-1 and albumin has also been studied. Despite the advances, the discovery of markers still remains a challenge as they do not show enough specificity to discern pancreatic cancer from other affections such as chronic pancreatitis. For this reason, there is still much study and subsequent validations to be carried out before achieving a sufficiently specific, sensitive, non-invasive and economically affordable biomarker for a more precise and early detection of pancreatic cancer
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2019-01-29 16:54:49.479
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oai:dugi-doc.udg.edu:10256/162712021-07-02T06:53:43Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Sabater, Sergi
Petrović, Mira
Mingorance García, Natalia
Universitat de Girona. Facultat de Ciències
2019-01-29T15:58:20Z
2019-01-29T15:58:20Z
2018-09
http://hdl.handle.net/10256/16271
Freshwater ecosystems continuously receive chemical stressors contributed by Wastewater Treatment Plant (WWTP) effluents. These effluents can produce important changes in receiving stream biological communities, particularly affecting their composition and functioning. One major impact of WWTP effluents into aquatic ecosystems is through river biofilms, which have a key role in the uptake and retention of inorganic and organic nutrients. Algae are part of river biofilms and rapidly respond to changes occurring in the river environment, making them very good indicators of the ecological status of river ecosystems. This study evaluates the effects of a long-term exposure of epilithic biofilm communities to a realistic urban WWTP effluent gradient (ranging from pure WWTP effluent to clean water) on the structure and composition of algal communities. The experiment was performed using artificial streams in a replicated regressional design. The initial hypotheses were that algal communities would: change as a result of the multiple stressors with an inverse relationship between diversity and pollution (i); the algal communities would shift towards tolerant taxa as a result of WWTP effluent exposure (ii); that algal communities would show a taxa succession during exposure and recovery phases, driving the community towards tolerant taxa during the exposure, and returning to the most sensitive taxa after recovery (iii). Results indicate that several taxa within the algal community were negatively affected by the WWTP effluents, while some others were favored. Overall, the algal diversity and taxa richness decreased at the end of the exposure and did not recover. The scores of the two first principal components of a PCA performed with the taxa abundance of algal communities were the best descriptors of the distribution patterns of the biological communities against the WWTP effluent exposure. Besides, these two principal components were fairly related with nitrites and DIN concentration. The results of the experiment allow to conclude that the environmental risk of WWTP effluents on the algal communities is high and produces long lasting and persistent effects
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Ecologia fluvial
Algues d'aigua dolça -- Efecte de la contaminació
Aigües residuals -- Depuració
Stream ecology
Biofilms
Freshwater algae -- Effect of pollution on
Sewage -- Purification
Biología
Efectes dels efluents de depuradora sobre els biofilms fluvials
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
text/plain
1748
https://dugi-doc.udg.edu/bitstream/10256/16271/3/license.txt
8a4605be74aa9ea9d79846c1fba20a33
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CC-LICENSE
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ORIGINAL
MingoranceGarcia.Natalia.pdf
MingoranceGarcia.Natalia.pdf
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2021-07-02 08:53:43.65
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oai:dugi-doc.udg.edu:10256/162722019-01-29T16:03:36Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Guasch i Padró, Helena
Pou Solà, Núria
Universitat de Girona. Facultat de Ciències
2019-01-29T16:03:36Z
2019-01-29T16:03:36Z
2018-09
http://hdl.handle.net/10256/16272
The main cyanobacteria that produces geosmin in freshwater ecosystems is Oscillatoria sp.. Geosmin is a molecule with organoleptic features without harmful effect on humans, but it produces a nuisance odour on spring seasonally. Determining the environmental conditions in which this organism appears will be useful to avoid the problem before it starts. This study aims to check the effect of light and water velocity on Oscillatoria sp. trough experimental channels. The advantage of those in front of in situ experiments is that the variables can be modified. The channels, located in Manlleu in the CERM-UVic centre, received water directly from the Ter river, where previously other studies had found geosmin. To define the effect of the choosen factors, cyanobacteria and algae identification, counting and measurement were performed using an optical microscope. The obtained data was converted to biovolume to obtain an easy interpretation, and they were analysed with RStudio program. The photonic efficiency experiment was also performed in the laboratory to verify the photosynthetic production of the biofilm in different known light intensities. Due to the latter results, we had learned under which treatments Oscillatoria sp. growth is favourable, and by exposing all the biofilm samples to a light intensity range and measuring its photonic efficiency, the P/I curves were elaborated. Those results will describe their production under each light intensity. With the obtained results, we can define the light and water velocity conditions favourable to Oscillatoria’s growth. Specifically it was confirmed that its presence is significant in low light and low velocity conditions. Also it wasn’t expected the significant growth in high light and 1m/s water velocity conditions. Although its photosynthetic production is anomalously low, compared to other treatments in the first favourable conditions described. This may be the reason that leads Oscillatoria sp. to produce geosmin
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cianobacteris -- Ecologia
Biofilms
Ecologia fluvial -- Catalunya -- Ter (Curs d'aigua)
Cyanobacteria -- Ecology
Stream ecology -- Catalonia -- Ter River
Geosmina
Biología
Influència de l'estrès múltiple sobre cianobacteris productors de geosmina
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.2852014000000054; north=42.0035444; name=Manlleu
NO
LICENSE
license.txt
license.txt
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2019-01-29 17:03:36.764
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oai:dugi-doc.udg.edu:10256/162732019-01-30T07:37:05Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Obradors Cherta, Albert
Pinart Nadal, Elisabeth
Puigmal Domínguez, Maria
Universitat de Girona. Facultat de Ciències
2019-01-30T07:37:05Z
2019-01-30T07:37:05Z
2018-09
http://hdl.handle.net/10256/16273
In the present study, a co-culturing method is proposed, which consists in culturing two embryos in the same plate. The aim of this study was to analyze the impact of embryo coculturing on the development and quality of human embryos, with the final goal to perform the Intra- cytoplasmic sperm injection procedure. An improvement of the embryo development and implantation rates was expected to achieve in those co-cultured embryos.
In order to analyze the co-culturing impact in the embryo features, morphological factors are evaluated in several embryo developmental stages. Monitoring these changing morphological events is currently feasible thanks to advanced strategies such as the timelapse incubator.
After the ovarian treatment, which included superovulation, ovarian puncture and removal of cumulus-corona, and the sperm selection, the injection procedure was carried out in a total of 297 oocytes from 21 patients. Then, morphological changes of the embryos were supervised and analyzed before proceeding to cryopreserve them. In this way, the transference of the best quality embryo could be performed and so, enhance the gestation and birth rates.
During the embryo development, a zygot scoring was applied to select those fertilized eggs with 2 pronuclei (2PN) and to discard the non-fertilized and degenerated oocytes, as well as those with abnormal fertilization. The fertilization rate of co-cultured embryos was higher than non-co-cultured embryos but non-statistically significant (85.05% and 78.64% respectively, P>0.05). No significant differences were shown neither at day 3 morphological evaluation nor embryo compaction at day 4 (P>0.05). Significantly (P<0.05) higher proportions of blastocysts at day 5 were obtained in the co-cultured embryos group, whereas at day 6 and day 7 blastocysts rates weren’t statistically different (P>0.05). For implantation rate, the hCG blood test and the analysis of the evolutive implantation weren’t significantly different (P>0.05), however the clinical implantation had significant differences (P<0.05) among cocultured and non-co- cultured embryos group.
It may be concluded that the co-culturing influenced beneficially the blastocyst percentages at day 5, as well as an improvement in the implantation rate. Hence, this new method might become a potential tool to improve the overall embryo development and so, benefit further assisted reproductive techniques
Submitted by M. LOURDES CATALÃ N (lourdes.catalan@udg.edu) on 2019-01-30T07:37:05Z
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application/pdf
eng
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Fecundació in vitro humana
Embrions -- Trasplantació
Embrions -- Crioconservació
Fertilization in vitro, Human
Embryo -- Cryopreservation
Embryo transplantation
Biología
Morphological study of human embryos co-culturing in a time-lapse incubator
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
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puigmaldominguez.maria.pdf
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2019-01-30 08:37:05.609
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oai:dugi-doc.udg.edu:10256/162742019-01-30T07:44:21Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Colprim Galceran, Jesús
Raventós Resina, Mireia
Universitat de Girona. Facultat de Ciències
2019-01-30T07:44:21Z
2019-01-30T07:44:21Z
2018-09
http://hdl.handle.net/10256/16274
Of all the existing mechanisms for the carbon dioxide fixation in the living beings, only the WoodLjungdahl (WLP) reductive pathway, used by acetogenic bacteria, sustains the two basic requirements for life: conservation of energy and the production of biomass. In this project, a prototype model is presented allowing the development of a methodology for the modeling of culture systems in a simulated bioreactor. To illustrate this methodology, a generic metabolic model of stationary state is developed in order to make predictions based on the environmental conditions. In addition, a biological study of the model bacteria (Clostridium ljungdahlii and Clostridium kluyveri) is performed to demonstrate that both bacteria have a Rnf complex responsible for the translocation of protons through the membrane that allows them to conserve energy.
The reductive pathway of CO2 leads to the formation of high energy density chemicals such as acetate, ethanol and butyrate. Metabolic reactions in this work take place at points very close to the thermodynamic equilibrium, which allows the acceptance of different assumptions. Therefore, the purpose of this work is based on the construction of a generic metabolic model of two model organisms (C. ljungdahlii and C. kluyveri) that describes the activation of different metabolic pathways depending on the environmental conditions of the cell. The results suggest that: (i) none of the reactions that take part in the carbon flow are directly responsible for the conservation of energy, which is why a complementary system –the Rnf complex– that connects the electronic transfer from reduced ferredoxin (Fd-2) to NAD +, is executed by carrying out a translocation of protons through the membrane (this same gradient of H+ is used by an ATPase to generate ATP); (ii) the only metabolic point that allows the conservation of energy is the Rnf complex; (iii) the total amount of energy obtained in the WL route in the form of ATP varies according to the involved enzymes proposed; (iv) C.ljungdahlii and C.kluyveri are considered as interesting organisms to be used as a microbial production platform; (v) there are certain parameters that directly affect the metabolic pathways and the results of the model.
Therefore, acetogenic bacteria are organisms of great study interest as they could be used as biocatalysts in the biotechnology industry, thanks to their capacity for fermentation of gases alike CO2
Submitted by M. LOURDES CATALÃ N (lourdes.catalan@udg.edu) on 2019-01-30T07:44:21Z
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Bacteris anaerobis -- Aplicacions industrials
Anaerobic bacteria -- Industrial applications
Biología
Construcció d’un model metabòlic genèric d’un microorganisme acetogènic del gènere clostridium
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
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8a4605be74aa9ea9d79846c1fba20a33
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2019-01-30 08:44:21.103
DUGiDocs
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oai:dugi-doc.udg.edu:10256/162772019-01-30T08:06:17Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Romaní i Cornet, Anna M.
Rebollo Pallejà, Ariadna
Universitat de Girona. Facultat de Ciències
2019-01-30T08:06:17Z
2019-01-30T08:06:17Z
2018-09
http://hdl.handle.net/10256/16277
Fine particulate matter plays an important role in stream ecological functioning, being a main driver of productivity and metabolism of fluvial ecosystems. A large portion of this fine particulate matter is deposited and accumulated in the hyporheic zone (the interface between groundwater and surface water). Therefore, it is important to understand the factors that determine the spatial distribution of this fine particulate matter and its bioreactivity. This is especially relevant to rivers that receive waste water treatment plant (WWTP) effluents because they are a constant source of fine particles, which can profoundly affect their metabolism. The objective of this work was to study the influence of the WWTP effluent of Santa Maria de Palautordera on the quantity, quality and bioreactivity of fine particulate matter in the receiving stream. In addition, we explored the physical and hydrological factors that determine its spatial distribution along a 100 m section downstream of the effluent inlet. The study was conducted at a time of storm flow conditions, which is an extreme hydrological moment, but at the same time very little studied. The results showed the same amount of fine particulate matter upstream and downstream of the effluent inlet from the WWTP, but higher percentage of organic matter downstream. The fine particulate matter along the reach was distributed heterogeneously, and we detected the presence of hot spots as a result of various factors, such as the spatial distribution of hydrological variables, as well as the presence of physical obstacles such as trunks and macrophyte patches. Microbial metabolic activity associated with fine particulate matter increased downstream of the WWTP effluent, especially at those points where fine particles rich in organic matter accumulated. This study shows that the influence of the WWTP on fine particle standing stocks and their bioreactivity is low during high flow conditions, when the dilution factor is high. However, fine particulate matter still tends to accumulate in specific stream areas (hot spots) even during moments of maximum advection and transport
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Ecologia fluvial -- Catalunya -- Tordera (Curs d'aigua)
Sediments fluvials -- Catalunya -- Tordera (Curs d'aigua)
Aigües residuals -- Depuració
Tordera (Catalunya : Curs d'aigua) -- Condicions mediambientals
River sediments -- Catalonia -- Tordera (River)
Stream ecology -- Catalonia -- Tordera (River)
Sewage -- Purification
Tordera (Catalonia : River) -- Environmental conditions
Biología
Distribució espacial de partícules fines bioreactives durant un moment de cabal elevat en un riu receptor d’aigua procedent d’una estació depuradora d’aigües residuals
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=2.640231299999982; north=41.69915779999999; name=Riu de la Tordera
NO
LICENSE
license.txt
license.txt
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RebolloPalleja.Ariadna.pdf
RebolloPalleja.Ariadna.pdf
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oai:dugi-doc.udg.edu:10256/162792019-01-30T08:16:13Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Heras Mena, Sandra
Rios Astorch, Carla
Universitat de Girona. Facultat de Ciències
2019-01-30T08:16:13Z
2019-01-30T08:16:13Z
2018-09
http://hdl.handle.net/10256/16279
Aristaeomorpha foliacea (Risso, 1827), also known as red shrimp, is a crustacean belonging to the Aristeidae family. It is a deep-water species with a global geographical distribution that inhabits in the seabed at a depth between 80 and 3.300m. Commercially, A. foliacea constitutes the basis of the trawling fisheries sector in many countries, especially on the Mediterranean coast.
In order to ensure the conservation of a species that inhabits at great depths and the continuity of it as a resource, genetics facilitates the study and allows it to be directed in the most diligent way: through molecular techniques, the genetic stocks can be precisely delimited, species can be identified and knowledge about the behaviour of the species, its natural history and current and past demographic factors can be provided in-depth. Thus, this study assesses the current state of genetic knowledge of A. foliacea and specifies the genetic areas on which further research efforts are needed in order to address the existing gaps.
The search of the bibliographic material was done through the scientific databases SCOPUS and WOS. First, the proper name of the species was used as a keyword and then, the articles were filtered selecting the categories with genetic, molecular and biochemical content. Once the articles were obtained, we analysed and assessed which ones were relevant for inclusion in this study. Thus, 7 articles were obtained and categorized in the thematic blocks of "Phylogenetic classifications", "Population analysis" and "Development of genetic tools".
The results of this work indicate the scarce effort that has been invested in the genetic research of this marine decapod and point out the areas that are still unknown, such as: providing a consensuated Penaeoidea and Aristeidae phylogeny; providing a consensuated Penaeoidea and Aristeidae phylogeny; discovering possible new genetic stocks throughout the entire distributional range of A. foliacea; stablishing the global microstructure within genetic populations of the species and testing the existing genetic tools.
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Aristaeomorpha foliacea
Gambes -- Genètica
Shrimps -- Genetics
Biología
Posada al dia dels estudis genètics de la gamba vermella Aristaeomorpha foliacea
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
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8a4605be74aa9ea9d79846c1fba20a33
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Rios Astorch, Carla.pdf
Rios Astorch, Carla.pdf
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oai:dugi-doc.udg.edu:10256/162802019-01-30T08:33:28Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Llorens i Guasch, Laura
Salicrú Benedet, Júlia
Universitat de Girona. Facultat de Ciències
2019-01-30T08:33:28Z
2019-01-30T08:33:28Z
2018-09
http://hdl.handle.net/10256/16280
A consequence of climate change will be a reduction in cloud cover and, therefore, an increase in ultraviolet radiation (UV) and a decrease in precipitation in the coming years. The main objective of this study was to investigate how UV radiation and precipitation affect the decomposition of the leaves of a Mediterranean plant, specifically Arbutus unedo. In addition, we also wanted to determine what are the main invertebrates that can be involved in this decomposition and if the composition of their community can be affected by changes in these climatic factors. The study was carried out for 8 months, from November to June, using 18 plots of 9 m2 exposed to 3 levels of UV radiation (UV-A radiation, UV-A radiation plus UV-B radiation and no radiation) and 2 of precipitation (natural precipitation and reduced precipitation). Different parameters were studied, such as the percentage of organic matter remaining, weight loss, enzyme activity and the content of C and N of the leaf litter. The research also included the study of the community of invertebrates present in leaf litter where we studied different ecological indexes and the abundance of the different orders. Neither the exposure to UV radiation, nor the reduction of precipitation affected negatively the enzyme activity of β-glucosidase. Regarding the percentage of organic matter remaining, at the end of the experiment (June), we saw that the decrease is higher when the litter is under reduced precipitation. In drought plots, the litter showed a greater weight loss when exposed to UV radiation. We have also observed that the content of C and N increases in respect to the initial values, especially in the case of the C within the plots with UV radiation and natural precipitation. On the other hand, the ratio of C:N decreases in respect to the initial values. Regarding the composition of the community on invertebrates, we have seen that the most abundant groups are mites, larvae and diptera. Although the treatments have not affected the ecological indexes studied, they have affected the relative abundance of some of the invertebrate groups
Submitted by M. LOURDES CATALÃ N (lourdes.catalan@udg.edu) on 2019-01-30T08:33:28Z
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application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Arbutus unedo
Fullaraca -- Biodegradació
Fullaraca -- Efecte de la radiació
Fullaraca -- Efecte de la sequera
Plant litter -- Effect of drought on
Plant litter -- Biodegradation
Plant litter -- Effect of radiation on
Biología
Efecte de la radiació ultraviolada (UV) i la sequera sobre les taxes de descomposició de les fulles d’Arbutus unedo
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
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oai:dugi-doc.udg.edu:10256/162822019-01-30T08:40:20Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Valiuska, Simonas
Universitat de Girona. Facultat de Ciències
2019-01-30T08:40:20Z
2019-01-30T08:40:20Z
2018-09
http://hdl.handle.net/10256/16282
The Roman villa of “Pla de Palol” in “Platja d'Aro” contains numerous malacological samples and its
study can provide both biological information about species diversity and information on the customs
of the Roman time. This project focuses in the identification of species, the study of exploitation of these
in the past compared to the present exploitation, analyse parameters of abundance and diversity to
compare it with the current situation. Another part of the study consists in the analysis of the most
probable techniques used to recollect the different molluscs considering the distribution of these. The
results reveal a high biodiversity, represented by 23 species, both bivalve and gastropods; some shells
have a clear manipulation suggesting several uses such as: the use of containers, culinary tools such as
spoons, skin scrapers or ornamental uses for the manufacture of necklaces, bracelets, etc. It has also
been found that the samples are represented equitably, except for Ostrea edulis with 185 samples, and
species such as Bolma rugosa or Cerastoderma glaucum have been found with only one simple sample.
The high presence of remains of species of gasterópodos of the Muricidae family (Hexaplex trunculus,
Bolinus brandaris and Stramonita haemastoma), fragmented or drilled, suggests a possible use for the
manufacture of purple dye, very valued by the Romans. Everything seems to indicate a current decrease
in the natural populations of species such as Ostrea edulis, Patella ferruginea or Spondylus gaederopus,
nowadays these species are described as vulnerable or endangered, on the other hand they were
present and abundant in the deposits. Most of the identified species are mainly found in the
mediolittoral zone and live in a sandy or rocky substratum. The lack of information in the fields of the
deposits makes it difficult to infer the possible uses of these, although some rooms such as 3 or 26 could
be dining rooms or kitchens, due to the abundance of samples and species, and others such as areas 14
or 15 could be waste areas where the shells of molluscs were thrown once consumed, as they are
supposedly outside of the built architectural structure
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cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Pla de Palol (Castell i Platja d'Aro, Catalunya : Jaciment arqueològic)
Mol·luscos -- Classificació -- Catalunya -- Pla de Palol
Mollusks -- Classification -- Catalonia -- Pla de Palol
Biología
Estudi de la fauna malacològica de la vil·la romana del Pla de Palol
info:eu-repo/semantics/bachelorThesis
Estudis de grau
east=3.0737837000000354; north=41.821777; name=Vil·la romana de Pla de Palol
NO
LICENSE
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oai:dugi-doc.udg.edu:10256/16282
2019-01-30 09:40:20.504
DUGiDocs
oriol.olive@udg.edu
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oai:dugi-doc.udg.edu:10256/162842019-01-30T08:46:51Zcom_10256_8023com_10256_2945col_10256_11211RC2openaire_dataRC1CIRAXRC4RC3driveropenaireMD2MD1recolecta
Massaguer i Vall-llovera, Anna
Tejerina Fontaiña, Patricia
Vilà i Quintana, Laura
Universitat de Girona. Facultat de Ciències
2019-01-30T08:46:51Z
2019-01-30T08:46:51Z
2018-09
http://hdl.handle.net/10256/16284
Introduction: Meningitis is a serious infection that in order to be treated efficiently needs a quick diagnosis. The current diagnosis is based on molecular biology and other microbiological techniques such as bacterial culture, which requires a lot of time and makes it difficult for an early diagnosis. For this reason, a new biomarker is sought especially in bacterial meningitis, since they are the ones that show a worse evolution in patient. We are looking for a specific and sensitive biomarker for cerebrospinal fluid (CSF) samples that give us relevant information for infections of the central nervous system (CNS). It has been shown that serum procalcitonin (PCT) is a protein that increases quantitatively in the presence of a bacterial infection. Objectives: To assess the use of PCT as a meningitis biomarker in CSF samples comparing it with other existing specific biomarkers: PCT in serum and lactate in CSF. Methods: From the clinical laboratory of the Hospital Universitari Doctor Josep Trueta for a period of six months, we collected data of PCT in serum and LCR, lactate, count of the cells and leukocyte, proteins and glucose in LCR of 48 patients with clinical suspicion of meningitis. We included patients with bacterial meningitis, either acquired in the community or post-surgical, viral meningitis and a control group without a disease and we compared the use of the biomarkers with ROC curves and Mann-Whitney tests. Results: The average concentration of PCT in CSF for the different groups studied was: 0.16 ng/mL (IQR=0.12, SD=0.13) for the bacterial meningitis group (n=7), 0.147 ng/mL (IQR=0, SD=NA) for the group of viral meningitis (n=1) and 0.52 ng/mL (IQR=0.11, SD=2.05) for the noninfectious group (n=37). The median differences (95%) between the bacterial and non-infectious group presented a p-value of: 0.7949 at PCT levels in CSF, 0.06184 at serum PCT levels and 0.008075 at levels of lactate in CSF. The results of the ROC curves comparing the bacterial group to the non-infectious group resulted in an AUC 0.9063 (95%, IC:0.7591-1.0534) for PCT in plasma, an AUC 0.5336 (95%, IC:0.287-0.7797) for PCT in CSF and an AUC 0.9167 (95%, CI:0.7661-1.0672) for lactate in CSF. Conclusions: It is suggested that PCT in LCR is not a good biomarker for the diagnosis of meningitis compared to the PCT in serum and lactate. In contrast, it is suggested that lactate is a valid biomarker for the diagnosis of bacterial meningitis
Submitted by M. LOURDES CATALÃ N (lourdes.catalan@udg.edu) on 2019-01-30T08:46:51Z
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Previous issue date: 2018-09
application/pdf
cat
Biologia (TFG)
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Líquid cefaloraquidi -- Anàlisi
Sistema nerviós central -- Infeccions -- Diagnòstic
Marcadors bioquímics
Cerebrospinal fluid -- Analysis
Central nervous system -- Infection -- Diagnosis
Biología
Utilitat de la procalcitonina en líquid cefaloraquidi en el diagnòstic d’infeccions del sistema nerviós central (SNC)
info:eu-repo/semantics/bachelorThesis
Estudis de grau
NO
LICENSE
license.txt
license.txt
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2019-01-30 09:46:51.417
DUGiDocs
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