Glycan Characterization of PSA 2-DE Subforms from Serum and Seminal Plasma
dc.contributor.author
dc.date.accessioned
2013-02-01T12:16:15Z
dc.date.available
2013-02-01T12:16:15Z
dc.date.issued
2010
dc.identifier.issn
1536-2310
dc.identifier.uri
dc.description.abstract
Prostate-specific antigen (PSA) two-dimensional electrophoresis (2-DE) subforms (F1–F5) have been described to be altered in prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH). To understand their molecular differences, characterization of these subforms from PCa serum and seminal plasma, namely, at the glycan level, was performed. PSA 2-DE subforms from two serum PCa samples and seminal plasma were analyzed by N-glycan sequencing using high-performance liquid chromatography (HPLC) combined with exoglycosidase array digestions and by mass spectrometry. F1, F2, and F3 subforms showed the same N-glycan pattern, which contained higher levels of sialic acid than the F4 subform, whereas the F5 subform was unglycosylated. When comparing PSA subforms from PCa with seminal plasma, a decrease in sialylation was observed. Furthermore, the analysis of F3, the more abundant PSA subform, showed a higher proportion of alpha 2–3 sialic acid and a decrease in core fucosylated glycans in the PCa sample. These N-glycan changes in PCa PSA subforms highlight the importance of glycosylation as an indicator of PCa disease
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Mary Ann Liebert, Inc
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1089/omi.2010.0050
dc.relation.ispartof
© OMICS A Journal of Integrative Biology, 2010, vol. 14, núm. 4, p. 465-474
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Tots els drets reservats. This is a copy of an article published in the OMICS A Journal of Integrative Biology © 2010 Mary Ann Liebert, Inc.; OMICS A Journal of Integrative Biology is available online at: http://www.liebertonline.com.
dc.subject
dc.title
Glycan Characterization of PSA 2-DE Subforms from Serum and Seminal Plasma
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
011716
dc.identifier.eissn
1557-8100