Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river

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dc.contributor.author Bonnineau, Chloé
dc.contributor.author Bonet Sánchez, Berta
dc.contributor.author Corcoll i Cornet, Natàlia
dc.contributor.author Guasch i Padró, Helena
dc.date.issued 2010
dc.identifier.citation Bonnineau, C., Bonet, B., Corcoll, N., i Guasch, H. (2010). Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river. Ecotoxicology, 20 (1), 293-303. Recuperat 27 octubre de 2011, a http://www.springerlink.com/content/c4372843g0854340/
dc.identifier.issn 0963-9292 (versió paper)
dc.identifier.issn 1573-3017 (versió electrònica)
dc.identifier.uri http://hdl.handle.net/10256/3673
dc.description.abstract Antioxidant enzymes are involved in important processes of cell detoxification during oxidative stress and have, therefore, been used as biomarkers in algae. Nevertheless, their limited use in fluvial biofilms may be due to the complexity of such communities. Here, a comparison between different extraction methods was performed to obtain a reliable method for catalase extraction from fluvial biofilms. Homogenization followed by glass bead disruption appeared to be the best compromise for catalase extraction. This method was then applied to a field study in a metal-polluted stream (Riou Mort, France). The most polluted sites were characterized by a catalase activity 4–6 times lower than in the low-polluted site. Results of the comparison process and its application are promising for the use of catalase activity as an early warning biomarker of toxicity using biofilms in the laboratory and in the field
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Springer
dc.relation.isformatof Versió postprint del document publicat a: http://dx.doi.org/10.1007/s10646-010-0564-2
dc.relation.ispartof © Ecotoxicology, 2010, vol. 20, núm. 1, p. 293-303
dc.relation.ispartofseries Articles publicats (D-CCAA)
dc.relation.uri http://hdl.handle.net/10803/36688
dc.rights Tots els drets reservats
dc.subject Biofilms
dc.subject Enzims
dc.subject Indicadors biològics
dc.subject Enzymes
dc.subject Indicators (Biology)
dc.title Catalase in fluvial biofilms: a comparison between different extraction methods and example of application in a metal-polluted river
dc.type info:eu-repo/semantics/article
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion
dc.identifier.doi http://dx.doi.org/10.1007/s10646-010-0564-2


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