A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)

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dc.contributor.author Viñas de Puig, Jordi
dc.contributor.author Tudela, Sergi
dc.date.issued 2009
dc.identifier.citation Viñas J., i Tudela, S. (2009). A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus). PLoS ONE, 4 (10), e7606. Recuperat 30 setembre de 2011,a doi:10.1371/ journal.pone.0007606
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10256/3594
dc.description.abstract Tuna species of the genus Thunnus, such as the bluefin tunas, are some of the most important and yet most endangered trade fish in the world. Identification of these species in traded forms, however, may be difficult depending on the presentation of the products, which may hamper conservation efforts on trade control. In this paper, we validated a genetic methodology that can fully distinguish between the eight Thunnus species from any kind of processed tissue. Methodology: After testing several genetic markers, a complete discrimination of the eight tuna species was achieved using Forensically Informative Nucleotide Sequencing based primarily on the sequence variability of the hypervariable genetic marker mitochondrial DNA control region (mtDNA CR), followed, in some specific cases, by a second validation by a nuclear marker rDNA first internal transcribed spacer (ITS1). This methodology was able to distinguish all tuna species, including those belonging to the subgenus Neothunnus that are very closely related, and in consequence can not be differentiated with other genetic markers of lower variability. This methodology also took into consideration the presence of introgression that has been reported in past studies between T. thynnus, T. orientalis and T. alalunga. Finally, we applied the methodology to cross-check the species identity of 26 processed tuna samples. Conclusions: Using the combination of two genetic markers, one mitochondrial and another nuclear, allows a full discrimination between all eight tuna species. Unexpectedly, the genetic marker traditionally used for DNA barcoding, cytochrome oxidase 1, could not differentiate all species, thus its use as a genetic marker for tuna species identification is questioned
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.isformatof Reproducció digital del document publicat a: http://dx.doi.org/10.1371/ journal.pone.0007606
dc.relation.ispartof PLoS ONE, 2009, vol.4, núm.10, p. e7606
dc.relation.ispartofseries Articles publicats (D-B)
dc.rights Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
dc.rights.uri http://creativecommons.org/licenses/by/2.5/es/deed.ca
dc.subject Tonyina -- Genètica
dc.subject Tuna -- Genetics
dc.title A Validated Methodology for Genetic Identification of Tuna Species (Genus Thunnus)
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0007606


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