Genotypic comparison of Pantoea agglomerans plant and clinical strains

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dc.contributor.author Rezzonico, Fabio
dc.contributor.author Smits, Theo H.M.
dc.contributor.author Montesinos Seguí, Emilio
dc.contributor.author Frey, Jürg E.
dc.contributor.author Duffy, Brion
dc.date.issued 2009
dc.identifier.citation Rezzonico, F., Smits, T.H.M., Montesinos,E., Frey, J.E., i Duffy, B. (2009). Genotypic comparison of Pantoea agglomerans plant and clinical strains. BMC Microbiology, 9, 204. Recuperat 29 juliol de 2011,a http://www.biomedcentral.com/1471-2180/9/204
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10256/3489
dc.description.abstract Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. Results: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion: Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this species
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.isformatof Reproducció digital del document publicat a: http://dx.doi.org/10.1186/1471-2180-9-204
dc.relation.ispartof BMC Microbiology, 2009, vol. 9, núm. 204
dc.relation.ispartofseries Articles publicats (D-EQATA)
dc.rights Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
dc.rights.uri http://creativecommons.org/licenses/by/2.0/es/deed.ca
dc.subject Bacteris -- Control biològic
dc.subject Plantes -- Malalties fúngiques
dc.subject Virosis (Plantes)
dc.subject Bacteria -- Biological control
dc.subject Fungal diseases of plants
dc.subject Virus diseases of plants
dc.title Genotypic comparison of Pantoea agglomerans plant and clinical strains
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1186/1471-2180-9-204


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