Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture

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dc.contributor.author Rabionet Díaz, Marc
dc.contributor.author Yeste Oliveras, Marc
dc.contributor.author Puig i Miquel, Teresa
dc.contributor.author Ciurana, Quim de
dc.date.issued 2017-08-01
dc.identifier.issn 2073-4360
dc.identifier.uri http://hdl.handle.net/10256/14368
dc.description.abstract In vitro cell culture is traditionally performed within two-dimensional (2D) environments, providing a quick and cheap way to study cell properties in a laboratory. However, 2D systems differ from the in vivo environment and may not mimic the physiological cell behavior realistically. For instance, 2D culture models are thought to induce cancer stem cells (CSCs) differentiation, a rare cancer cell subpopulation responsible for tumor initiation and relapse. This fact hinders the development of therapeutic strategies for tumors with a high relapse percentage, such as triple negative breast cancer (TNBC). Thus, three-dimensional (3D) scaffolds have emerged as an attractive alternative to monolayer culture, simulating the extracellular matrix structure and maintaining the differentiation state of cells. In this work, scaffolds were fabricated through electrospinning different poly("-caprolactone)-acetone solutions. Poly("-caprolactone) (PCL) meshes were seeded with triple negative breast cancer (TNBC) cells and 15% PCL scaffolds displayed significantly (p < 0.05) higher cell proliferation and elongation than the other culture systems. Moreover, cells cultured on PCL scaffolds exhibited higher mammosphere forming capacity and aldehyde dehydrogenase activity than 2D-cultured cells, indicating a breast CSCs enrichment. These results prove the powerful capability of electrospinning technology in terms of poly("-caprolactone) nanofibers fabrication. In addition, this study has demonstrated that electrospun 15% PCL scaffolds are suitable tools to culture breast cancer cells in a more physiological way and to expand the niche of breast CSCs. In conclusion, three-dimensional cell culture using PCL scaffolds could be useful to study cancer stem cell behavior and may also trigger the development of new specific targets against such malignant subpopulation
dc.description.sponsorship This work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P; RYC-2014-15581), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher MDPI (Multidisciplinary Digital Publishing Institute)
dc.relation MINECO/PE 2014-2016/DPI2013-45201-P
dc.relation.isformatof Reproducció digital del document publicat a: https://doi.org/10.3390/polym9080328
dc.relation.ispartof Polymers, 2017, vol. 9, núm. 8, p. 328
dc.relation.ispartofseries Articles publicats (D-EMCI)
dc.rights Attribution 4.0 Spain
dc.rights.uri http://creativecommons.org/licenses/by/4.0/es/
dc.subject Mama -- Càncer
dc.subject Breast -- Cancer
dc.subject Materials nanoestructurats
dc.subject Nanostructured materials
dc.subject Polímers en medicina
dc.subject Polymers in medicine
dc.subject Nanotecnologia
dc.subject Nanotechnology
dc.title Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture
dc.type info:eu-repo/semantics/article
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.embargo.terms Cap
dc.type.version info:eu-repo/semantics/publishedVersion
dc.identifier.doi https://doi.org/10.3390/polym9080328
dc.contributor.funder Ministerio de Economía y Competitividad (Espanya)

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