Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
dc.contributor.author
dc.date.accessioned
2017-06-22T11:39:08Z
dc.date.available
2017-06-22T11:39:08Z
dc.date.issued
2017-03-30
dc.identifier.issn
2045-2322
dc.identifier.uri
dc.description.abstract
Understanding the interplay between molecules and lipid membranes is fundamental when studying
cellular and biotechnological phenomena. Partition between aqueous media and lipid membranes is
key to the mechanism of action of many biomolecules and drugs. Quantifying membrane partition,
through adequate and robust parameters, is thus essential. Surface Plasmon Resonance (SPR) is a
powerful technique for studying 1:1 stoichiometric interactions but has limited application to lipid
membrane partition data. We have developed and applied a novel mathematical model for SPR data
treatment that enables determination of kinetic and equilibrium partition constants. The method uses
two complementary fitting models for association and dissociation sensorgram data. The SPR partition
data obtained for the antibody fragment F63, the HIV fusion inhibitor enfuvirtide, and the endogenous
drug kyotorphin towards POPC membranes were compared against data from independent techniques.
The comprehensive kinetic and partition models were applied to the membrane interaction data of
HRC4, a measles virus entry inhibitor peptide, revealing its increased affinity for, and retention in,
cholesterol-rich membranes. Overall, our work extends the application of SPR beyond the realm of 1:1
stoichiometric ligand-receptor binding into a new and immense field of applications: the interaction of
solutes such as biomolecules and drugs with lipids
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.relation.isformatof
Reproducció digital del document publicat a: https://doi.org/10.1038/srep45647
dc.relation.ispartof
Scientific Reports, 2017, vol.7, art. núm. 45647
dc.relation.ispartofseries
Articles publicats (D-Q)
dc.rights
Attribution 3.0 Spain
dc.rights.uri
dc.subject
dc.title
Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi