Lysozyme enhances the bactericidal effect of BP100 peptide against Erwinia amylovora, the causal agent of fire blight of rosaceous plants
dc.contributor.author
dc.date.accessioned
2017-06-15T08:20:39Z
dc.date.available
2017-06-15T08:20:39Z
dc.date.issued
2017-02-17
dc.identifier.issn
1471-2180
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dc.description.abstract
Background: Fire blight is an important disease affecting rosaceous plants. The causal agent is the bacteria Erwinia
amylovora which is poorly controlled with the use of conventional bactericides and biopesticides. Antimicrobial
peptides (AMPs) have been proposed as a new compounds suitable for plant disease control. BP100, a synthetic
linear undecapeptide (KKLFKKILKYL-NH2), has been reported to be effective against E. amylovora infections.
Moreover, BP100 showed bacteriolytic activity, moderate susceptibility to protease degradation and low toxicity.
However, the peptide concentration required for an effective control of infections in planta is too high due to
some inactivation by tissue components. This is a limitation beause of the high cost of synthesis of this compound.
We expected that the combination of BP100 with lysozyme may produce a synergistic effect, enhancing its activity
and reducing the effective concentration needed for fire blight control.
Results: The combination of a synhetic multifunctional undecapeptide (BP100) with lysozyme produces a
synergistic effect. We showed a significant increase of the antimicrobial activity against E. amylovora that was
associated to the increase of cell membrane damage and to the reduction of cell metabolism. Combination of
BP100 with lysozyme reduced the time required to achieve cell death and the minimal inhibitory concentration
(MIC), and increased the activity of BP100 in the presence of leaf extracts even when the peptide was applied at
low doses. The results obtained in vitro were confirmed in leaf infection bioassays.
Conclusions: The combination of BP100 with lysozyme showed synergism on the bactericidal activity against E.
amylovora and provide the basis for developing better formulations of antibacterial peptides for plant protection
dc.description.sponsorship
This work has been supported by the project AGL2009-13255-C02-01/AGR from MICINN of Spain. The research group has been supported in part by CIRIT of the Catalonian Government (Ref. 2009SGR812) and by the University of Girona
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application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.relation
info:eu-repo/grantAgreement/MICINN//AGL2009-13255-C02-01/ES/Control Biotecnologico Del Fuego Bacteriano.Utilizacion De Cepas De Bacterias Gram-Positivas Productoras De Bacteriocinas Y Ciclolipopeptidos/
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Reproducció digital del document publicat a: http://dx.doi.org/10.1186/s12866-017-0957-y
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BMC Microbiology, 2017, vol. 17, p. 39
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Articles publicats (D-EQATA)
dc.rights
Attribution 3.0 Spain
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dc.subject
dc.title
Lysozyme enhances the bactericidal effect of BP100 peptide against Erwinia amylovora, the causal agent of fire blight of rosaceous plants
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
026608
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dc.relation.ProjectAcronym