Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
dc.contributor.author
dc.date.accessioned
2016-12-02T07:56:13Z
dc.date.available
2016-12-02T07:56:13Z
dc.date.issued
2015
dc.identifier.uri
dc.description.abstract
The antibacterial activity against bacterial plant pathogens and its relationships with the
presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin),
fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide
products have been studied in a collection of 64 strains of Bacillus spp. isolated from plant
environments. The most frequent antimicrobial peptide (AMP) genes were bmyB, srfAAand
fenD (34-50% of isolates). Most isolates (98.4%) produced surfactin isoforms, 90.6%iturins
and 79.7% fengycins. The antibacterial activity was very frequent and generally intense
among the collection of strains because 75% of the isolates were active against at least 6 of
the 8 bacterial plant pathogens tested. Hierarchical and correspondence analysis confirmed
the presence of two clearly differentiated groups. One group consisted of Bacillus strains
that showed a strong antibacterial activity, presented several cLPs genes and produced
several isoforms of cLPs simultaneously, mainly composed of B. subtilisand B. amyloliquefaciens,
although the last one was exclusive to this group. Another group was characterized
by strains with very low or none antibacterial activity, that showed one or none of the cLP
genes and produced a few or none of the corresponding cLPs, and was the most heterogenous
group including B. subtilis, B. licheniformis, B. megaterium, B. pumilus, B. cereusand
B. thuringiensis, although the last two were exclusive to this group. This work demonstrated
that the antagonistic capacity of plant-associated Bacillusagainst plant pathogenic bacteria
is related to the presence of cLP genes and to the production of the corresponding cLPs,
and it is mainly associated to the species B. subtilisand B. amyloliquefaciens. Our findings
would help to increase the yield and efficiency of screening methods to obtain candidate
strains to biocontrol agents with a mechanism of action relaying on the production of antimicrobial
cLPs
dc.description.sponsorship
Funding was provided by Programa Nacional de Agricultura y Alimentación (CICYT) (Ref. AGL-2009-13255-C02-01) of the MINECO of Spain, and FP7 KBBE.2013.1.2-04. Ref. 613678. DROPSA project of the European Union. The research group is accredited and supported in part by SGR 2009-0812, 2014 SGR 697 and TECNIO net from Catalonia
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science (PLoS)
dc.relation
info:eu-repo/grantAgreement/MICINN//AGL2009-13255-C02-01/ES/Control Biotecnologico Del Fuego Bacteriano.Utilizacion De Cepas De Bacterias Gram-Positivas Productoras De Bacteriocinas Y Ciclolipopeptidos/
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.1371/journal.pone.0127738
dc.relation.ispartof
PLoS One, 2015, vol. 10, núm. 5, p. e0127738
dc.relation.ispartofseries
Articles publicats (D-EQATA)
dc.rights
Attribution 4.0 Spain
dc.rights.uri
dc.subject
dc.title
Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.relation.projectID
info:eu-repo/grantAgreement/EC/FP7/613678/EU/Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens/DROPSA
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
024696
dc.contributor.funder
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
1932-6203