Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin
dc.contributor.author
dc.date.accessioned
2016-09-09T07:44:12Z
dc.date.available
2016-09-09T07:44:12Z
dc.date.issued
2015-03
dc.identifier.issn
0009-8981
dc.identifier.uri
dc.description.abstract
Pancreatic adenocarcinoma (PDAC) usually shows an enhanced expression of sialyl-Lewis X (sLex) and related epitopes. PDAC may secrete some of the proteins carrying such increased sLex determinant into serum, so they could be used as PDAC markers. Previously, we identified acute-phase proteins with increased sLex in both PDAC and in chronic pancreatitis patients. In this study, depleted sera from the main acute-phase proteins has been analysed for the search of proteins with increased sLex levels in PDAC. Sera from healthy controls, chronic pancreatitis and PDAC patients were depleted, electrophoresed and subjected to sLex immunodetection. Proteins that differentially expressed sLex in PDAC were trypsin digested and identified by LC-ESI-QTOF mass spectrometry. Five protein bands that differentially expressed sLex in PDAC were identified and corresponded to seven different acute-phase proteins. Among them, ceruloplasmin (CP) was selected for further analysis. N-glycan sequencing of CP confirmed the increase of sLex levels in CP in PDAC patients. Healthy controls, chronic pancreatitis and PDAC patients' sera were immunoprecipitated with anti-CP antibodies, and their sLex and CP levels were analysed by western blot. The sLex/CP ratio tended to be higher for the PDAC group, which altogether suggests that the sLex/CP ratio could be a useful biomarker for PDAC
dc.description.sponsorship
This work was supported by the Spanish Ministry of Science and Innovation, grants BIO 2007-61323 and BIO 2010-16922, awarded to R. P., and by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 260600 (“GlycoHIT”), awarded to R. S.
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
info:eu-repo/grantAgreement/MICINN//BIO2010-16922/ES/GLICOSILACION ALTERADA EN CANCER: GLICOPROTEINAS Y GLICOSILTRANSFERASAS EN SUERO Y EN TEJIDOS COMO MARCADORES TUMORALES/
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Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.cca.2015.01.007
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© Clinica Chimica Acta, 2015, vol. 442, p. 56-62
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Articles publicats (D-B)
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Tots els drets reservats
dc.title
Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
020287
dc.contributor.funder
dc.relation.ProjectAcronym