Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality
dc.contributor.author
dc.date.accessioned
2016-02-08T08:22:11Z
dc.date.available
2016-02-08T08:22:11Z
dc.date.issued
2016
dc.identifier.issn
0378-4320
dc.identifier.uri
dc.description.abstract
The present study sought to determine the relationship of three sperm proteins (acrosin binding protein, ACRBP; outer dense fibre protein 1, ODF1; and triosephosphate isomerase, TPI), and two seminal plasma proteins (fibronectin, FN1; and epididymal secretory glutathione peroxidase, GPX5) to conventional sperm quality parameters (sperm membrane integrity, morphology and motility) in pigs. With this purpose, 22 boar ejaculates were split into two groups according to their sperm quality (mean ± standard error of the mean, % viable sperm: 95.25 ± 0.53 vs. 78.22. ± 1.93; % morphologically normal sperm: 96.30 ± 0.66 vs 80.81 ± 2.28). The amounts of these five proteins were evaluated through Western blot analysis and subsequently compared between these two groups through a t-test for independent samples. Normalised levels of TPI in sperm were significantly higher in the low than in the high sperm quality group. In addition, TPI was found to be negatively correlated with sperm membrane integrity, morphology and several parameters describing sperm motility. On the other hand, amounts of GPX5 in seminal plasma were also significantly higher in the low than in the high quality group, and this protein was also found to be negatively correlated with sperm membrane integrity and total and progressive sperm motility. By contrast, sperm content in ACRBP and ODF1 amounts of seminal plasma protein FN1 did not significantly differ between the two groups of sperm quality. Thus, we can conclude that sperm TPI content and amounts of GPX5 in seminal plasma may be used as quality markers of boar sperm
dc.description.sponsorship
AcknowledgementsThe authors acknowledge the support from RegionalGovernment of Catalonia (Generalitat de Catalunya, Spain;Grant code: 2014-SGR-1230) and Ministry of Economyand Competitiveness, Spain (Grant code: AGL2013-47798-P). N.P.M. was supported by a PhD Studentship fundedby the Regional Government of Catalonia (Generalitat deCatalunya). M.Y. is funded by the European Commission,FP7-People Programme, Marie Curie-IEF (Grant Number:626061)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Masson
dc.relation
info:eu-repo/grantAgreement/MINECO//AGL2013-47798-P/ES/EVALUACION DE LOS PEPTIDOS ANTIMICROBIANOS COMO SUSTITUTOS DE LOS ANTIBIOTICOS EN LOS DILUYENTES DE REFRIGERACION DEL SEMEN PORCINO/
AGAUR/2014-2016/2014 SGR 1230
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Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.anireprosci.2015.12.001
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© Animal Reproduction Science, 2016, vol. 165, p. 22-30
dc.relation.ispartofseries
Articles publicats (D-B)
dc.rights
Tots els drets reservats
dc.subject
dc.title
Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/embargoedAccess
dc.embargo.terms
Cap
dc.date.embargoEndDate
info:eu-repo/date/embargoEnd/2026-01-01
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
024455
dc.contributor.funder
dc.relation.ProjectAcronym
dc.identifier.eissn
1873-2232