Impact of formate on the growth and productivity of Clostridium ljungdahlii PETC and Clostridium carboxidivorans P7 grown on syngas
dc.contributor.author
dc.date.accessioned
2015-10-16T11:23:07Z
dc.date.available
2015-10-16T11:23:07Z
dc.date.issued
2014
dc.identifier.issn
1139-6709
dc.identifier.uri
dc.description.abstract
The current energy model based on fossil fuels is coming to an end due to the increase in global energy demand. Biofuels such as ethanol and butanol can be produced through the syngas fermentation by acetogenic bacteria. The present work hypothesizes that formate addition would positively impact kinetic parameters for growth and alcohol production in Clostridium ljungdahlii PETC and Clostridium carboxidivorans P7 by diminishing the need for reducing equivalents. Fermentation experiments were conducted using completely anaerobic batch cultures at different pH values and formate concentrations. PETC cultures were more tolerant to formate concentrations than P7, specially at pH 5.0 and 6.0. Complete growth inhibition of PETC occurred at sodium formate concentrations of 30.0 mM; however, no differences in growth rates were observed at pH 7.0 for the two strains. Incubation at formate concentrations lower than 2.0 mM resulted in increased growth rates for both strains. The most recognizable effects of formate addition on the fermentation products were the increase in the total carbon fixed into acids and alcohols at pH 5.0 and pH 6.0, as well as, a higher ethanol to total products ratio at pH 7.0. Taken all together, these results show the ability of acetogens to use formate diminishing the energy demand for growth, and enhancing strain productivity
dc.description.sponsorship
The authors thank the Autonomous Government of Catalonia (Generalitat de Catalunya) (2013 FI-DGR) and the Spanish Ministry of Science and Innovation (Best-Energy, CTQ2011-23632, CTM2013-43454-R) for their financial support in this study. LEQUIA and IEA have been recognized as consolidated research groups by the Catalan Government (2014-SGR-1168 and 2014-SGR-2016). RG gratefully acknowledges support from Beatriu de Pinos fellowship (BP-2011-B) and FP7 Marie Curie Career Integration Grants (PCIG13-GA-2013-618593)
dc.format.mimetype
application/pdf
dc.language.iso
eng
dc.publisher
Spanish Society for Microbiology (SEM)
dc.relation
info:eu-repo/grantAgreement/MINECO//CTM2013-43454-R/ES/CARACTERIZACION GENETICA Y FUNCIONAL DE MICROORGANISMOS CARBOXIDOTROFICOS EN SISTEMAS BIOELECTROQUIMICOS PARA LA ASIMILACION DE CO2/
info:eu-repo/grantAgreement/MICINN//CTQ2011-23632/ES/SISTEMAS BIOELECTROQUIMICOS PARA EL TRATAMIENTO DE AGUAS: DE LA TRANSFERENCIA ELECTRONICA EXTRACELULAR A LA APLICACION BIOTECNOLOGICA/
AGAUR/2014-2016/2014 SGR 1168
AGAUR/2014-2016/2014 SGR 2016
dc.relation.isformatof
Reproducció digital del document publicat a: http://dx.doi.org/10.2436/20.1501.01.222
dc.relation.ispartof
© International Microbiology, 2015, vol. 17, núm. 4, p. 195-204
dc.relation.ispartofseries
Articles publicats (D-EQATA)
dc.rights
Attribution-NonCommercial-ShareAlike 3.0 Spain
dc.rights.uri
dc.subject
dc.title
Impact of formate on the growth and productivity of Clostridium ljungdahlii PETC and Clostridium carboxidivorans P7 grown on syngas
dc.type
info:eu-repo/semantics/article
dc.rights.accessRights
info:eu-repo/semantics/openAccess
dc.embargo.terms
Cap
dc.relation.projectID
info:eu-repo/grantAgreement/EC/FP7/618593/EU/Biological production of butanol from syngas/SYNTOBU
dc.type.version
info:eu-repo/semantics/publishedVersion
dc.identifier.doi
dc.identifier.idgrec
022267
dc.contributor.funder
dc.relation.FundingProgramme
dc.relation.ProjectAcronym
dc.identifier.eissn
1618-1905